Induced Pluripotent Stem Cells Core
iPSC reprogramming overview. From PBMCs (top), from Fibroblasts (bottom)
Induced pluripotent stem cells are a novel resource for studying disease mechanisms and advancing personalized medicine, driving the demand for a dedicated Induced Pluripotent Stem Cells (iPSC) Core at Nationwide Children's Hospital.
Our goal is to generate and share iPSC lines with investigators not only at Nationwide Children's and The Ohio State University, but throughout Ohio and across the country. Our experienced staff are here to help during every step of your project, ranging from PBMC isolation and reprogramming to enrichment training and obtaining MTAs. We also provide characterization, cryopreservation and differentiation services on newly generated iPSC lines and existing lines from a congenital heart disease iPSC biorepository. Please reach out to our team for more information.
Resources Available
We provide the following resources to investigators and staff looking to integrate iPSCs into their research. We would be happy to provide you with the materials and documents you need to successfully conduct iPSC research.
- Protocols for iPSC generation and differentiation
- Existing iPSC lines from a congenital heart disease iPSC biorepository
- Administrative and legal support
Services We Offer
- Peripheral blood mononuclear cell (PBMC) isolation
- Human iPSC line generation and cryopreservation
- Quality control of iPSC lines including karyotyping, pluripotency marker expression, in vitro germ layer differentiation, cell line authentication, and mycoplasma contamination testing
- Cardiomyocyte and Endothelial cell differentiation
- Enrichment training for iPSC culture and differentiation
A wide range of services are offered to investigators, staff, and trainees. If you're interested or have any questions, please click the button below and complete the form at the bottom of the webpage.
Join Us!
Email us at Mingtao.Zhao@nationwidechildrens.org or stop by W0045!
Upcoming Activities and Events
iPSC Core "Grand Opening"
June 18 - 25
More details to come!
Featured Publications
- Generation of an induced pluripotent stem cell line (NCHi023-A) from a 41-year-old male with nemaline myopathy carrying autosomal dominant ACTA1 c.809-10C>A mutation
- Generation of an induced pluripotent stem cell line (NCHi016-A) from a 5-year-old female with pulmonary atresia with intact ventricular septum and one-and-half ventricle palliation
- Generation of iPSC line NCHi015-A from a patient with truncus arteriosus carrying heterozygous variants in KMT2D and NOTCH1
- Creation of iPSC line NCHi004-A from a patient with down syndrome and congenital heart defects
- Establishment of NCHi009-A, an iPSC line from a patient with hypoplastic left heart syndrome (HLHS) carrying a heterozygous NOTCH1 mutation
