Flow Cytometry and Immune Monitoring Core

Flow Cytometry has a diverse array of applications and the Abigail Wexner Research Institute (AWRI) seeks to help researchers with any application they wish to pursue. For trained investigators, we provide technical expertise and 24/7 access to equipment to facilitate your research with minimal oversight. Our knowledgeable staff are here if needed to assist you with experiment design, data acquisition, data analysis and any other questions you may have. For investigators that require a higher level of service, the Flow Cytometry Core now offers full-service support of research studies requiring routine PBMC processing, cryopreservation, T cell ELISPOT analysis, multiplexing or immunophenotyping.

Equipment
Services
Training
Scheduling
Policies
Publications
Collaborations
Immune Monitoring

Equipment

  • Cytek Aurora, 5 lasers
  • BD LSR Fortessa, 5 lasers
  • BD LSR II Cytometer, 3 lasers
  • Beckman CytoFLEX LX, 4 lasers
  • BD Influx Cell Sorter, 5 lasers
  • BD Melody Cell Sorter, 3 lasers
  • Nanosight NS300
  • Biotek Synergy LX Plate Reader
  • Bio-Plex 200 Array System
  • SYSMEX Cell Counter

Pricing is available on iLabs or upon request through email.

Services

  • Cell sorting, $66/hr
  • Operator-assisted acquisition, $75/hr
  • Bioplex service, $200/assay + consumables
  • Consultation, $50/hr

Training

Faculty, staff, and students of the Abigail Wexner Research Institute at Nationwide Children’s research community are permitted to use the Flow Cytometry Facility. Prior to independent use of any of the cytometers, every user is required to attend an in-service training session with one of the core facility staff.

For training, please contact Dave Dunaway and Cecele Denman

Scheduling

Flow Cytometry sessions on any of the analytical cytometers are scheduled on a first come first serve basis by signing up via the Flow Cytometry core service request link. Access to the eCores reservation system is predicated upon completion of the required initial training session for all new cytometer users. In addition you must have an eCores account in order to access the reservation system. All cytometers are available to investigators 24 hours/day, 7 days/week. Once the required training session has been completed new users will be provided with a user name and password in order to use the cytometers.

Policies

Click here to download our policies.

Publications

Click here to view our publications.

Collaborations

Click here to view our collaborations.

Services

  • PBMC Isolation and Cryopreservation
  • Plasma/Serum Separation
  • Splenocyte Processing
  • IFN-y ELISpots
  • AAV/Transgene Antibody ELISAs
  • Vector Shedding PCR
  • Multiplex Assay
  • DNA Extractions
  • Equipment Use
  • Training
  • Consultations
The Immune Monitoring Core is a centralized core laboratory within AWRI that offers immune monitoring services for rAAV Gene Therapy Pre-Clinical Studies and Phase I Trials, cell-based therapy studies and other projects upon request. Services are performed in accordance with academic research standards, but do not fulfill all criteria for 21 CRF 58 definition of GLP for non-clinical studies, OECD Test Guidelines or CAP/CLIA accreditation.

Requests

Email our team at ImmuneMonitoring@NationwideChildrens.org with details about your research project (potential start date, sample type, assay, etc.) and a team member will connect and guide you through next steps.