Cardiovascular Research Shared Resource Core

Cardiovascular Research Shared Resource Core, known as Heart Center Molecular Analysis Core, is home to state-of-the-art molecular biology equipment and provides a wide range of services including determination of circulating and tissue biomarker levels, gene expression analysis, tissue biorepository development, along with molecular biology consultation.

Mission

  • Support clinical and basic science faculty
  • Design and implement a variety of molecular assays
  • Accelerate translational research

Examples of our Research

Example 1
Example 2
Example 3
Example 4

Biomarker Analysis

The core offers a wide range of high-sensitive assays for human serum, plasma, urine, and saliva sample analysis. We have participated in NIH-funded, drug-company-sponsored and NCH-funded projects. Some representative examples are below.

  • Identifying Kidney Injury via Urinary Biomarkers after the Comprehensive Stage II Palliation and Bidirectional Glenn procedure. Rachel Harris, Tyler Cunningham, Christina Phelps, Diana Zepeda-Orozco, Jianli Bi, Brian Beckman, Robin Alexander, Catherine D. Krawczeski, and Andrew R. Yates, Presented at the PCICS annual meeting, Miami, FL, 2022.

Changes of NGAL in patients undergoing BDG or CS2 palliation

  • Utility of sTREM-1 and Presepsin to Predict New Infection on Mechanical Circulatory Support. Robert Murray, Jianli Bi, Brian Beckham, Joshua Frazier and Andrew Yates. Presented at SCCM Congress, San Francisco, CA, 2023

research example

Biorepository

Centralized repository to inventory, archive and distribute existing tissue and body fluid samples in low (-20C) or ultralow (-80C) tempature. We provided heart tissue samples for the following representative publications.

  • Nitric oxide prevents aortic valve calcification by S-nitrosylation of USP9X to activate NOTCH signaling. Uddalak Majumdar, Talita Z. Choudhury, Sathiyanarayanan Manivannan, Yukie Ueyama, Madhumita Basu and Vidu Garg. Sci Adv. 2021 Feb 5;7(6): eabe3706

Graph

  • Novel frameshift variant in MYL2 reveals molecular differences between dominant and recessive forms of hypertrophic cardiomyopathy. Manivannan SN, Darouich S, Masmoudi A, Gordon D, Zender G, Han Z, Fitzgerald-Butt S, White P, McBride KL, Kharrat M, Garg V. PLoS Genet. 2020;16(5).

Biorepository

Gene Expression

We provide faculty with comprehensive and cost-competitive genetic analyses focused on next-generation sequencing for basic and translational cardiovascular research.

  • Impaired Human Cardiac Cell Development due to NOTCH1 Deficiency. Shiqiao Ye, Cankun Wang, Zhaohui Xu, Hui Lin, Xiaoping Wan, Yang Yu, Subhodio Adhicary, Joe Z Zhang, Yang Zhou, Chun Liu, Matthew Alonzo, Jianli Bi, Angelina Ramirez-Navarro, Isabelle Deschenes, Qin Ma, Vidu Garg, Joseph C Wu, Ming-Tao Zhao. Circ Res. 2023 Jan 20;132(2): 187-204

Single cell transcription analysis

Biology Consultation and Training

Biology consultation and training: Consultation services include project initiation, cost estimation, instruction of sample collection and processing, and experimental trouble shooting. Training services include ELISA, PCR, western blotting, immunochemistry, sample preparation for 10x single cell RNA sequencing.  

Lab Staff

Jianli Bi, MD, PhD
Senior Research Scientist
Core Manager