Genomic Services
This Genomics Services Laboratory (GSL) has over a decade of experience with next-generation sequencing (NGS) data generation and analysis. The GSL provides service to both internal and external investigators and can assist with multiple aspects of NGS, including experimental design, library preparation, sequence generation, and data analysis. Please contact Amy Wetzel, PhD, at Amy.Wetzel@NationwideChildrens.org or (614) 355-3530 or email IGMGenomicServices@NationwideChildrens.org for questions regarding instrumentation and service offerings.
Illumina
- NovaSeq6000
- NextSeq2000
- iSeq
- iScan
PacBio
- Sequel IIe
- Revio
Singular Genomics
- G4 sequencer
10x Genomics
- Chromium X
- CytAssist
NanoString
- nCounter prep station + scanner
- GeoMx Digital Spatial Profiler
Other
- Hamilton STARlet liquid handler
- Thermo Fisher Bigfoot Spectral Cell Sorter
- Sage Science Blue Pippin
- Agilent 4200 TapeStation and 2100 Bioanalyzer
- Qiagen QIAcube Connect and EZ1
- Bionano Saphyr
- Miltenyi gentleMACS Octo Dissociator
- Invitrogen Qubit Fluorometer
- Genome sequencing
- Exome sequencing (human; mouse)
- RNA/miRNA sequencing (human; mouse; rat; bacteria)
- PacBio HiFi, Iso-seq, Plasmid prep, Kinnex single cell
- DNA and RNA extractions
- 10x Genomics Single Cell (Gene expression; ATAC; Fixed RNA)
- 10x Genomics Visium Spatial Gene Expression
- Nucleic Acid QC (TapeStation; Qubit; Bioanalyzer)
- Bioinformatics services (inquire)
- GSL current pricing for NCH and external academic users is attached. Please inquire for pricing if you are an external industry/non-academic customer.
How to Submit Samples
1. Initiate and submit your request via iLab.
- Login or Register for an iLab account, https://nch.corefacilities.org/landing/19
- Under Core Facilities, select Nationwide Children’s Hospital (NCH) and Genomics Services Laboratory (GSL)
- Initiate the Service Request
- Enter the project description information on NGS Form
- The GSL will issue a quote upon completion of your request
- Agree to quote and provide payment information
- You must have payment information supplied before work on samples will begin
2. Complete Project Intake Form (PIF) and Sample Submission Form (SSF) at this link: IGM PIF and SSF
Of note, the SSF should only be completed once the iLab order has been submitted.
3. Non-NCH users must also sign a master service agreement (MSA) or confirm with the GSL that an institutional MSA is currently in place and attach a hard copy of purchase order (PO) to their iLab order prior to completing the SSF.
4. Ship or deliver your samples. If delivering your samples in person, please contact IGM Genomic Services @ IGM Genomic Services IGMGenomicServices@nationwidechildrens.org to schedule a specific day and time. The Genomic Services Laboratory location is in Research Building 3, WB2160, on NCH main campus.
If shipping samples, please ship to the address below.
Institute for Genomic Medicine
Attn: IGM Research Sample Receipt and Accessioning Team
Nationwide Children’s Laboratory Services
700 Children’s Drive, Room C1955
Columbus, OH 43205 U.S.A.
Additionally, please send the FedEx tracking number and include a shipping manifest with sample information (identifiers, specimen type, etc.) to the IGM Research Sample Receipt and Accessioning group email listed above to ensure efficient receipt and triaging of samples.
Please email IGMResearchSampleReceiptandAccessioning@nationwidechildrens.org if you have questions regarding specimen submission for extraction services, or shipment of samples.
Hours of Operation
Regular hours are 8:00 a.m. to 4:30 p.m., Monday through Friday.
Sample Submission Guidelines
Please submit samples in Eppendorf 1.5 ml LoBind tubes (Fisher Sci; Cat#13-698-791), or Eppendorf™ twin.tec™ 96 Well LoBind PCR Plates, Skirted (Fisher Sci; Cat# E0030129512). Tubes should be clearly labeled and readable with GSL order number, sample identifier, and lab name/PI.
For RNA/DNA QC services, submit samples in 0.5ml PCR strip tubes.
Samples may be suspended in dH2O, 10mM Tris, or Qiagen EB. High salt (EDTA) concentration can interfere with the various enzymes that are used during library preparation. Our preference is that samples are suspended in dH2O.
The IGM Computational Genomics Group has partnered with the GSL to provide the substantial technical and bioinformatics expertise required to oversee the multiple platforms that acquire, store and analyze large and complex genomic data sets. The Translational Bioinformatics Team provides bioinformatics services on a collaborative basis at subsidized hourly rates and serves as an interface between the research investigator and the multiple domains that are required to handle the size and complexity of genomic data. IGM has built a dedicated, cloud-based computational resource through Amazon Web Services (AWS), allowing large HPC or Hadoop clusters with hundreds of instances to be provisioned on demand, enabling us to support the analysis needs of both large and small scale sequencing projects. We are uniquely positioned to adapt and develop new computational tools in a manner that is driven by the demands of our research community as it utilizes new technologies.
The Computational Genomics Group has developed state of the art analytical pipelines for NGS analysis including, but not limited to, transcriptomics (RNA-Seq and miRNA-Seq), whole genome sequencing (WGS), whole exome sequencing (WES) and paired tumor-normal somatic variant detection. Through development of the balanced parallelization strategy for human genome analysis (named “Churchill”), we were successfully able to reduce the time taken to go from raw sequencer output to final variant calls from days to under two hours, enabling both rapid and accurate identification of disease causing genetic variants in germline and cancer samples. In addition to developing our own bioinformatics tools, the unit also evaluates and supports multiple software products for genomics applications that are designed to make data analysis tools accessible to biologists.
The Computational Genomics Group is a dynamic team of computational biologists, with the substantial technical and bioinformatics expertise required to oversee the multiple platforms that acquire, store and analyze large and complex data sets generated by the Steve and Cindy Rasmussen Institute for Genomic Medicine (IGM).
Technology and applications development is an essential component of the Steve and Cindy Rasmussen Institute for Genomic Medicine vision. This group evaluates emerging, cutting-edge methods and instrumentation that improve current data production and analysis methods.
Methods: “[Service] was performed by the IGM Genomic Services Lab of the Abigail Wexner Research Institute at Nationwide Children’s Hospital, Columbus, Ohio”
Acknowledgements: “We thank [name(s) of personnel] of the IGM Genomic Services Lab of the Abigail Wexner Research Institute at Nationwide Children’s Hospital, Columbus, Ohio for their help with [help provided]”