Mary Cismowski, PhD

Mary Cismowski, PhD is a principal investigator in the Center for Cardiovascular and Pulmonary Research at The Research Institute at Nationwide Children’s Hospital. She is a Research Associate Professor of Pediatrics at the Ohio State University College of Medicine.

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Languages Spoken:

• English

Research Center:

• Center for Cardiovascular and Pulmonary Research

Areas of Interest:

• Our group serves as a molecular screening core for the Heart Center. In this capacity we have developed a variety of assays to examine circulating biomarkers, gene expression changes and post-translational changes that occur in response to inherited and acquired cardiovascular diseases. In addition to this work, our group is studying the function of a small family of heterotrimeric G-protein regulators in cardiovascular and other diseases. One of these regulators, termed AGS1, is upregulated in vascular smooth muscle cells following hypoxic stress. We speculate that this regulator functions to modulate proliferation and/or migration of these cells during neovascularization.
• Development and standardization of biochemical and molecular biology assays to identify novel genetic and epigenetic regulators of pediatric cardiovascular and pulmonary diseases
• Continued characterization of signaling pathways regulated by the heterotrimeric G-protein modulators AGS1 and AGS8; analysis of their function in cardiovascular disease and cardiovascular development
• Development of high throughput genetic screening methodologies for the rapid identification of GPCR and heterotrimeric G-protein signal regulators from normal and diseased tissues

Undergraduate School

• Occidental College,
  Date Completed: 06/30/1983

Post Doctoral

• The Johns Hopkins University
  Date Completed: 06/30/1990

Fellowship

• The Scripps Research Institute, Department of Molecular Biology,
  Date Completed: 06/30/1994

Fellowship

• The University of Illinois at Chicago, Laboratory for Molecular Biology,
  Date Completed: 06/30/1997

2008–present

• Principal Investigator/Laboratory Coordinator, Center for Cardiovascular and Pulmonary Research, The Research Institute at Nationwide Children’s Hospital, Columbus, OH.
  

2003–2008

• Associate Professor, Biochemistry and Pharmacology, Department of Integrative Medical Sciences, NEOUCOM, Rootstown, OH.

2002–2003

• Associate Scientist and Director, Laboratory for Cellular Signaling, The Guthrie Research Institute, Sayre, PA.

2000–2002

• Senior Scientist, Molecular Biology, Neurocrine Biosciences, Inc., San Diego, CA.  Supervisor:  Richard Maki, Ph.D., Director

1999–2000

• Research Investigator, Pharmacology, OSI Pharmaceuticals, Tarrytown, NY.  Supervisor: Arlindo Castelhano, Ph.D., Executive Director.  (Note: In 1999, OSI Pharmaceuticals acquired Cadus Pharmaceutical Corporation)

1999–2000

• Consultant, Proteome Incorporated, Beverly, MA

1997–1999

• Scientist II, Receptor Pharmacology/Biochemistry, Cadus Pharmaceutical Corporation, Tarrytown, NY.  Supervisor: Emir Duzic, Ph.D., D.V.M, Associate Director

• Hutchinson, KR, Guggilam, A, Cismowski, MJ, Galantowicz, ML, West, TA, Stewart, JA, Zhang, XJ, Lord, KC, Lucchesi, PA. 2011. Temporal pattern of left ventricular structural and functional remodeling following reversal of volume overload heart failure.  JOURNAL OF APPLIED PHYSIOLOGY. Vol. 111, no. 6. (December): 1778-1788.
• Katz,Paige,S; Trask,Aaron,J; Souza-Smith,Flavia,M; Hutchinson,Kirk,R; Galantowicz,Maarten,L; Lord,Kevin,C; Stewart,James,A; Cismowski,Mary,J; Varner,Kurt,J; Lucchesi,Pamela,A. 2011. Coronary arterioles in type 2 diabetic (db/db) mice undergo a distinct pattern of remodeling associated with decreased vessel stiffness.  BASIC RESEARCH IN CARDIOLOGY. Vol. 106, no. 6. (November): 1123-1134.
• Perez,Jessica; Torres,Rebecca,A; Rocic,Petra; Cismowski,Mary,J; Weber,David,S; Darley-Usmar,Victor,M; Lucchesi,Pamela,A. 2011. PYK2 signaling is required for PDGF-dependent vascular smooth muscle cell proliferation.  AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY. Vol. 301, no. 1. (July): C242-C251.
• Sato,Motohiko; Hiraoka,Masahiro; Suzuki,Hiroko; Bai,Yunzhe; Kurotani,Reiko; Yokoyama,Utako; Okumura,Satoshi; Cismowski,Mary,J; Lanier,Stephen,M; Ishikawa,Yoshihiro. 2011. Identification of Transcription Factor E3 (TFE3) as a Receptor-independent Activator of G alpha(16) GENE REGULATION BY NUCLEAR G alpha SUBUNIT AND ITS ACTIVATOR.  JOURNAL OF BIOLOGICAL CHEMISTRY. Vol. 286, no. 20. (May): 17766-17776.
• Chatterjee S; Wang JW; Cismowski MJ; Bower JR; Rosenthal KS. 2009. HSV-2 ICP34.5 protein modulates herpes simplex virus glycoprotein processing.  Archives Of Virology. Vol. 154, no. 4. (January): 661-663.
• Chatterjee S, Wang JW, Cismowski MJ, Bower JR, Rosenthal KS.  HSV-2 ICP34.5 protein modulates herpes simplex virus glycoprotein processing.  Arch Virol. 2009;154(4):661-3. Epub 2009 Mar 7.
  PubMed ID: 19267180
• Lanier, S.M., Struckhoff, A. and Cismowski, M.J. 2007. Activator of G Protein Signaling 1. San Diego, CA: UCSD-Nature Molecule Pages (doi:10.1038/mp.a000226.01).
• Cismowski MJ. 2006. Non-receptor activators of heterotrimeric G-protein signaling (AGS proteins).  Seminars In Cell & Developmental Biology. Vol. 17, no. 3. (June): 334-344.
• Cismowski, M.J. 2006. Genistein. xPharm. New York, NY: Elsevier, Inc..
• Sato, M., Cismowski, M.J., Toyota, E., Smrcka, A.V., Lucchesi, P.A., Chilian, W.M. and Lanier, S.M.  (2006) Identification of a receptor-independent activator of G-protein signaling (AGS8) in ischemic heart and its interaction with Gbg.  Proc. Natl. Acad. Sci. USA 103:797-802. PubMed ID: 16407149
• Sato M; Cismowski MJ; Toyota E; Smrcka AV; Lucchesi PA; Chilian WM; Lanier SM. 2006. Identification of a receptor-independent activator of G protein signaling (AGS8) in ischemic heart and its interaction with Gbetagamma.  Proceedings Of The National Academy Of Sciences Of The United States Of America. Vol. 103, no. 3. (January): 797-802.
• Cismowski, M.J. 2006. Tyrosine kinase inhibitors. xPharm. New York, NY: Elsevier, Inc..
• Blumer JB; Cismowski MJ; Sato M; Lanier SM. 2005. AGS proteins: receptor-independent activators of G-protein signaling.  Trends In Pharmacological Sciences. Vol. 26, no. 9. (September): 470-476.
• Cismowski MJ; Lanier SM. 2005. Activation of heterotrimeric G-proteins independent of a G-protein coupled receptor and the implications for signal processing.  Reviews Of Physiology, Biochemistry And Pharmacology. Vol. 155, no. January: 57-80.
• Cismowski, M.J. and Lanier, S.M.  (2005) Activation of heterotrimeric G-proteins independent of a G-protein coupled receptor and the implications for signal processing.  Rev. Physiol. Biochem. Pharmacol. 155:57-80. PubMed ID: 16041530
• Blumer, J.B., Cismowski, M.J., Sato, M. and Lanier, S.M. (2005) AGS proteins diversify G-protein signaling.  Trends Pharmacol. Sci. 26:470-476. PubMed ID: 16084602
• Vaidyanathan G; Cismowski MJ; Wang G; Vincent TS; Brown KD; Lanier SM. 2004. The Ras-related protein AGS1/RASD1 suppresses cell growth.  Oncogene. Vol. 23, no. 34. (July): 5858-5863.
• Cao X; Cismowski MJ; Sato M; Blumer JB; Lanier SM. 2004. Identification and characterization of AGS4: a protein containing three G-protein regulatory motifs that regulate the activation state of Gialpha.  The Journal Of Biological Chemistry. Vol. 279, no. 26. (June): 27567-27574.
• Cepoi D; Phillips T; Cismowski M; Goodfellow VS; Ling N; Cone RD; Fan W. 2004. Assessment of a small molecule melanocortin-4 receptor-specific agonist on energy homeostasis.  Brain Research. Vol. 1000, no. 1-2. (March): 64-71.
• Cepoi, D., Phillips, T., Cismowski, M., Goodfellow, V.S., Ling, N., Cone, R.D. and Fan, W.  (2004) Assessment of a small molecule melanocortin-4 receptor-specific agonist on energy homeostasis.  Brain Res. 1000:64-71. PubMed ID: 15053953
• Cismowski, M.J. 2004. Imatinib xPharm. New York, NY: Elsevier, Inc..
• Cao, X., Cismowski, M.J., Sato, M., Blumer, J.B. and Lanier, S.M.  (2004) Identification and characterization of AGS4: A GPR protein that regulates the activation state of Gia.  J. Biol. Chem. 279:27567-27574. PubMed ID: 15096500
• Vaidyanathan, G., Cismowski, M.J., Wang, G., Vincent, T.S., Brown, K.D. and Lanier, S.M.  (2004) The Ras-related protein AGS1/RASD1 suppresses cell growth.  Oncogene 23:5858-5863. PubMed ID: 15184869
• Dyck B; Parker J; Phillips T; Carter L; Murphy B; Summers R; Hermann J; Baker T; Cismowski M; Saunders J; Goodfellow V. 2003. Aryl piperazine melanocortin MC4 receptor agonists.  Bioorganic & Medicinal Chemistry Letters. Vol. 13, no. 21. (November): 3793-3796.
• Foster AC; Joppa M; Markison S; Gogas KR; Fleck BA; Murphy BJ; Wolff M; Cismowski MJ; Ling N; Goodfellow VS; Chen C; Saunders J; Conlon PJ. 2003. Body weight regulation by selective MC4 receptor agonists and antagonists.  Annals Of The New York Academy Of Sciences. Vol. 994, no. June: 103-110.
• Nickolls SA; Cismowski MI; Wang X; Wolff M; Conlon PJ; Maki RA. 2003. Molecular determinants of melanocortin 4 receptor ligand binding and MC4/MC3 receptor selectivity.  The Journal Of Pharmacology And Experimental Therapeutics. Vol. 304, no. 3. (March): 1217-1227.
• Dyck, B., Parker, J., Phillips, T., Carter, L., Murphy, B., Summers, R., Hermann, J., Baker, T., Cismowski, M., Saunders, J. and Goodfellow, V.  (2003) Aryl piperazine melanocortin MC4 receptor agonists.  Bioorg. Med. Chem. Lett. 13:3793-3796. PubMed ID: 14552781
• Nickolls, S.A., Cismowski, M., Wang, X., Wolff, M., Conlon, P. and Maki, R.A.  (2003) Molecular determinants of melanocortin 4 receptor ligand binding and MC4/MC3 receptor selectivity.  J. Pharmacol. Exp. Ther., 304:1217-1227. PubMed ID: 12604699
• Foster, A.C., Joppa, M., Markison, S., Gogas, K.R., Fleck, B.A., Murphy, B.J., Wolff, M., Cismowski, M.J., Ling, N., Goodfellow, V.S., Chen, C., Saunders, J. and Conlon, P.J.  (2003) Body weight regulation by selective MC4 receptor agonists and antagonists.  Ann. NY Acad. Sci. 994:103-110. PubMed ID: 12851304
• Takesono A; Nowak MW; Cismowski M; Duzic E; Lanier SM. 2002. Activator of G-protein signaling 1 blocks GIRK channel activation by a G-protein-coupled receptor: apparent disruption of receptor signaling complexes.  The Journal Of Biological Chemistry. Vol. 277, no. 16. (April): 13827-13830.
• Cismowski MJ; Takesono A; Ma C; Lanier SM; Duzic E. 2002. Identification of modulators of mammalian G-protein signaling by functional screens in the yeast Saccharomyces cerevisiae.  Methods In Enzymology. Vol. 344, no. January: 153-168.
• Cismowski MJ; Metodiev M; Draper E; Stone DE. 2001. Biochemical analysis of yeast G(alpha) mutants that enhance adaptation to pheromone.  Biochemical And Biophysical Research Communications. Vol. 284, no. 2. (June): 247-254.
• Cismowski MJ; Takesono A; Bernard ML; Duzic E; Lanier SM. 2001. Receptor-independent activators of heterotrimeric G-proteins.  Life Sciences. Vol. 68, no. 19-20. (April): 2301-2308.
• Cismowski MJ; Ma C; Ribas C; Xie X; Spruyt M; Lizano JS; Lanier SM; Duzic E. 2000. Activation of heterotrimeric G-protein signaling by a ras-related protein. Implications for signal integration.  The Journal Of Biological Chemistry. Vol. 275, no. 31. (August): 23421-23424.
• Takesono A; Cismowski MJ; Ribas C; Bernard M; Chung P; Hazard S 3rd; Duzic E; Lanier SM. 1999. Receptor-independent activators of heterotrimeric G-protein signaling pathways.  The Journal Of Biological Chemistry. Vol. 274, no. 47. (November): 33202-33205.
• Cismowski MJ; Takesono A; Ma C; Lizano JS; Xie X; Fuernkranz H; Lanier SM; Duzic E. 1999. Genetic screens in yeast to identify mammalian nonreceptor modulators of G-protein signaling.  Nature Biotechnology. Vol. 17, no. 9. (September): 878-883.
• Li E; Cismowski MJ; Stone DE. 1998. Phosphorylation of the pheromone-responsive Gbeta protein of Saccharomyces cerevisiae does not affect its mating-specific signaling function.  Molecular & General Genetics: MGG. Vol. 258, no. 6. (June): 608-618.
• Cismowski MJ; Laff GM; Solomon MJ; Reed SI. 1995. KIN28 encodes a C-terminal domain kinase that controls mRNA transcription in Saccharomyces cerevisiae but lacks cyclin-dependent kinase-activating kinase (CAK) activity.  Molecular And Cellular Biology. Vol. 15, no. 6. (June): 2983-2992.
• Stueland CS; Lew DJ; Cismowski MJ; Reed SI. 1993. Full activation of p34CDC28 histone H1 kinase activity is unable to promote entry into mitosis in checkpoint-arrested cells of the yeast Saccharomyces cerevisiae.  Molecular And Cellular Biology. Vol. 13, no. 6. (June): 3744-3755.
• Cismowski MJ; Narula SS; Armitage IM; Chernaik ML; Huang PC. 1991. Mutation of invariant cysteines of mammalian metallothionein alters metal binding capacity, cadmium resistance, and 113Cd NMR spectrum.  The Journal Of Biological Chemistry. Vol. 266, no. 36. (December): 24390-24397.
• Cismowski MJ; Huang PC. 1991. Effect of cysteine replacements at positions 13 and 50 on metallothionein structure.  Biochemistry. Vol. 30, no. 26. (July): 6626-6632.
• Cismowski MJ; Huang PC. 1991. Purification of mammalian metallothionein from recombinant systems.  Methods In Enzymology. Vol. 205, no. January: 312-319.
• Huang, P.C., Cody, C., Cismowski, M., Chernaik, M., Rhee, I-K., and Lin, L.Y. 1991. Native and Engineered Metallothioneins. In Metallothionein in Biology and Medicine. Edited by C.D. Klaassen and K.T. Suzuki. Boston, MA: CRC Press, Inc..
• Cismowski, M., Chernaik, M., Rhee, I-K. and Huang, P.C. 1988. Expression of a Chinese hamster metallothionein coding sequence and its variants in S. cerevisiae. In Metal Ion Homeostasis: Molecular Biology and Chemistry. Edited by D. Winge and D. Hamer. New York, NY: Alan R. Liss, Inc..
• Pine R; Cismowski M; Liu SW; Huang PC. 1985. Construction and characterization of a library of metallothionein coding sequence mutants.  DNA (Mary Ann Liebert, Inc.). Vol. 4, no. 2. (April): 115-126.