Leif D. Nelin, MD

Dr. Nelin is the Division Chief of Neonatology at Nationwide Children’s Hospital and a Professor of Pediatrics at The Ohio State University College of Medicine. Clinically, Dr. Nelin is particularly interested in respiratory diseases, specifically in how pulmonary blood flow affects newborn respiratory diseases. Thus, persistent pulmonary hypertension of the newborn(PPHN) is one of his primary clinical interests. This has lead to his research interest in nitric oxide (NO), a potent vasodilator whose production is decreased in patients with PPHN. Utilizing cell culture techniques, isolated perfused lungs and animal preparations, Dr. Nelin studies the mechanisms that determine biological availability of the amino acid L-arginine on the ability of the lungs to produce NO and thereby regulate blood flow. The ultimate goal of this research is to understand the pathophysiology of PPHN and to develop new and specific therapies for the treatment of lung diseases that are characterized by alterations in NO production. A second area of research interest is the study of the developmental toxicology of inhaled NO.

Gender:

• Male

Languages Spoken:

• English
• Swedish

Research Center:

• Center for Perinatal Research

Areas of Interest:

• The research focus in my laboratory is on the elucidation of the functional and mechanistic aspects of cellular uptake of L-arginine by the cationic amino acid transporters (CAT) and the role of CAT activities and expression in regulation of nitric oxide (NO) production in the lung. Furthermore, we are studying the regulation of nitric oxide synthase and arginase induction in models of pulmonary hypertension and lung injury to develop strategies to influence arginine metabolism inside of the cell. Experiments are carried out in cell culture systems and animal models using pharmacological and genetic manipulations with experimental end-points ranging from biochemical assays, protein and mRNA assays, to functional and physiological measurements. The long-term goals of these studies are to develop therapies that increase NO production by facilitating CAT-mediated L-arg uptake in pulmonary hypertensive diseases, and that decrease NO production by inhibiting CAT-mediated L-arg uptake in inflammatory lung diseases.

Research Funding*:

• The Role of MKP-1 in Innate Immune Responses to bacterial cell wall components, NIH/NIAID
• Cationic Amino Acid Transporters and Lung NO Production, National Heart, Lung and Blood Institute

Undergraduate

• Florida Atlantic University
  Date Completed: 06/30/1979

Medical School

• University of South Florida
  Date Completed: 06/12/1982

Internship

• Baylor College of Medicine
  Date Completed: 06/30/1983

Residency

• Baylor College of Medicine
  Date Completed: 06/30/1985

Residency

• Uppsala University
  Date Completed: 01/01/1986

Fellowship

• Baylor College of Medicine
  Date Completed: 06/30/1988

Department:

• Pediatrics

Section:

• Neonatology

Speciality:

• Neonatal-Perinatal Medicine, Certified
  
• Pediatrics
  

Date of Appointment at Children's Hospital:

• 05/23/2003

2010–present

• Interim Chief, Division of Neonatology, The Ohio State University

2007–present

• Director, Center for Perinatal Research, The Research Institute at Nationwide Children's Hospital, Columbus, OH

2002–present

• Associate Professor, Department of Pediatrics, Ohio State University, Columbus, Ohio

2006–2007

• Interim Director, Center for Perinatal Research Nationwide Children's Research Institute, Columbus, Ohio

2002–2006

• Investigator, Center for Developmental Pharmacology and Toxicology, Columbus Children's Research Institute, Columbus, OH

1998–2002

• Associate Professor, Department of Pediatrics, University of New Mexico, Albuquerque, NM

1994–1998

• Associate Professor, Department of Pediatrics, Medical college of Wisconsin, Milwaukee, WI

1994–1998

• Adjunct Associate Professor, Biomedical engineering Department, Marquette University, Milwaukee, WI

1989–1994

• Assistant Professor, Department of Pediatrics, Medical College of Wisconsin, Milwaukee, WI

1989–1994

• Adjunct Assistant Professor, Biomedical Engineering Department, Marquette University, Milwaukee, WI

1988–1989

• Instructor, Department of Pediatrics, Medical College of Wisconsin, Milwaukee, WI

• Stenger MR, Rose MJ, Joshi MS, Rogers LK, Chicoine LG, Bauer JA, Nelin LD. 2010. Inhaled nitric oxide prevents 3-nitrotyrosine formation in the lungs of neonatal mice exposed to >95% oxygen.  Lung. Vol. 3, no. 188 (June): 217-227.
• Rose MJ; Stenger MR; Joshi MS; Welty SE; Bauer JA; Nelin LD. 2010. Inhaled nitric oxide decreases leukocyte trafficking in the neonatal mouse lung during exposure to >95% oxygen.  Pediatric Research. Vol. 67, no. 3. (March 1): 244.
• Jin Y, Calvert TJ, Chen B, Chicoine LG, Joshi MS, Bauer JA, Liu Y, Nelin LD. Mice deficient in Mkp-1 develop more severe pulmonary hypertension and greater lung protein levels of arginase in response to chronic hypoxia.  Am J Physiol Heart Circ Physiol. (In press)
• Toby IT, Chicoine LG, Cui H, Chen B, Nelin LD. Hypoxia-induced proliferation of human pulmonary microvascular endothelial cells depends on epidermal growth factor receptor tyrosine kinase activation.  Am J Physiol Lung Cell Mol Physiol. (In press)
• Nankervis CA, Martin EM, Crane ML, Samson KS, Welty SE, Nelin LD. 2010. Implementation of a multidiscplinary guideline-driven approach to the care of the extremely premature infant improved hospital outcomes.  Acta Paediatr. Vol. 2, no. 99 (February): 188-193.
• Robert G Castile and Leif D Nelin. 2010. Lung function, structure and the physiologic basis for mechanical ventilation of infants with established BPD. In Brochopulmonary Dysplasia. Edited by Steven H Abman. New York: Lippincott. (Formally Accepted)
• Frazier WJ; Wang X; Wancket LM; Li XA; Meng X; Nelin LD; Cato AC; Liu Y. 2009. Increased inflammation, impaired bacterial clearance, and metabolic disruption after gram-negative sepsis in Mkp-1-deficient mice.  Journal Of Immunology (Baltimore, Md.: 1950). Vol. 183, no. 11. (December 1): 7411.
• Chen B; Calvert AE; Cui H; Nelin LD. 2009. Hypoxia promotes human pulmonary artery smooth muscle cell proliferation through induction of arginase.  American Journal Of Physiology. Lung Cellular And Molecular Physiology. Vol. 297, no. 6. (December 1): L1151.
• Nankervis Ca, Martin EM, Crane ML, Samson KS, Welty SE, Nelin LD. Implementation of a multidisciplinary guideline-driven approach to the care of the extremely premature infant improved hospital outcomes.  Acta Paediatr. (In press)
• Wang X; Zhao Q; Matta R; Meng X; Liu X; Liu CG; Nelin LD; Liu Y. 2009. Inducible nitric-oxide synthase expression is regulated by mitogen-activated protein kinase phosphatase-1.  The Journal Of Biological Chemistry. Vol. 284, no. 40. (October 2): 27123.
• Matta R, Wang X, Ge H, Ray W, Nelin LD, Liu Y. 2009. Triptolide induces anti-inflammatory cellular responses.  Am J Transl Res. Vol. 3, no. 1. (March 5): 267-282.
• Tipple TE, SE Welty, LD Nelin, JM Hansen and LK Rogers. Alterations of the Thioredoxin System by Hyperoxia: Implications for Alveolar Development. Am J Respir Cell Mol Biol. (In press)
• Malleske DT, LG Chicoine and LD Nelin. 2009. pro-proliferative endothelial cell phenotype occurs late in the course of inflammation [Abstract]. FASEB J. Vol. 1024, no. 23: 2 (Published)
• Undem C, S Pisarcik, LD Nelin and LA Shimoda. 2009. Differential effect of nicotine on intracellular calcium concentration ([Ca2+]i) in human umbilical artery and vein endothelial cells [Abstract]. FASEB J. Vol. 807, no. 23: 9 (Published)
• Jin Y, TJ Calvert, LG Chicoine and LD Nelin. 2009. Chronic hypoxia decreases arterial compliance in rat lungs [Abstract]. FASEB J. Vol. 619, no. 23: 3 (Published)
• Wang X, JW Frazier, X Meng, LD Nelin and Y Liu. 2009. function of MAP kinase phosphatase-1 in Gram-negative sepsis. [Abstract]. FASEB J. Vol. 710, no. 23: 5 (Published)
• Han Z, AJ Pope, AJ Cardounel, LD Nelin and LG Chicione. 2009. Increased ADMA/protein methylation modulates nitric oxide generation in hypoxic human lung microvascular endothelial cells [Abstract]. FASEB J. Vol. 1024, no. 23: 6 (Published)
• Cua CL, AL Cooper, MA Stein, RJ Corbitt and LD Nelin. Tissue Doppler Changes in Three Neonates with Congenital Diaphragmatic Hernia. ASAIO J. (In press)
• Alexander LJ, PJ Giannone, CA Nankervis, LG Chicoine, CP Richardson and LD Nelin. 2009. Successful extubation of mechanically ventilated NICU patients is not related to ventilator pressures. [Abstract]. J Perinatol. Vol. 26: 582 (Published)
• Matta R, X Wang, H Ge, W Ray, LD Nelin and Y Liu. The anti-inflammatory mechanism of triptolide. AM J Transl Res. (In press)
• Toby IT, H Cui, B Chen and LD Nelin. 2009. Hypoxia-induced proliferation via epidermal growth factor receptor signaling in pulmonary endothelial cells [Abstract]. FASEB J. Vol. 1024, no. 23: 5 (Published)
• Rogers LK, TE Tipple, LD Nelin and SE Welty. 2009. Differential responses in the lungs of newborn mouse pups exposed to 85% or >95% oxygen. Pediatr Res. Vol. 65: 33-38.
• Chang R, Chicoine LG, Cui H, Kanagy NL, Walker BR, Liu Y, English BK, Nelin LD. 2008. Cytokine-induced arginase activity in pulmonary endothelial cells in dependent on Src family tyrosine kinase activity. Am J Physiol Lung Cell Mol Physiol.. Vol. 295, no. 4. (October): L688-L697. (IF: 4.214)
• Wang X, Nelin LD, Kuhlman JR, Meng X, Welty SE, Liu Y. 2008. The role of MAP kinase phosphatase-1 in the protective mechanism of dexamethasone against endotoxemia. Life Sci.. Vol. 82, no. 19-20. (September): 671-680. (IF: 2.257)
• Allahdadi KJ, Cherng TW, Pai H, Silva AQ, Walker BR, Nelin LD, Kanagy NL. 2008. A receptor antagonist normalizes blood pressure in rats exposed to eucapnic intermittent hypoxia. Am J Physiol Heart Circ Physio.. Vol. 295, no. 1. (July): H434-H440. (IF: 3.973)
• Calvert TJ, Chicoine LG, Liu Y, Nelin LD. 2008. Deficiency of mitogen-activiated protein kinase phosphatase-1 results in iNOS-mediated hypotension in response to low-dose endotoxin. Am J Physiol Heart Circ Physiol.. Vol. 294, no. 4. (April): H1621-H1629. (IF: 3.973)
• Jadcherla SR, Gupta A, Fernandez S, Nelin LD, Castile R, Gest AL, Welty S. 2008. Spatiotemporal characteristics of acid refluxate and relationship to symptoms in premature and term infants with chronic lung disease. Am J Gastroenterol.. Vol. 103, no. 3. (March): 720-728. (IF: 6.101)
• Nelin LD, TJ Calvert, LG Chicoine and Y Liu. 2008. Lipopolysaccharide-induced nitric oxide production and hypotension in Mkp-1 deficient mice depends on L-arginine uptake [Abstract]. FASEB J. Vol. 749, no. 22: 11 (Published)
• Cui H, LG Chicoine, Y Liu and LD Nelin. 2008. The cytotoxic effects of inducible nitric oxide synthase depend on L-arginine uptake in pulmonary arterial endothelial cells [Abstract]. PAS. Vol. 5854: 11 (Published)
• Tipple TE, LD Nelin, SE Welty and LK Rogers. 2008. Pulmonary VEGF and thioredoxin interacting protein levels are inversely correlated in a neonatal mouse model of BPD [Abstract]. PAS. Vol. 3245: 1 (Published)
• Toby IT, LG Chicoine, H Cui and LD Nelin. 2008. Hypoxia-induced proliferation of human pulmonary microvascular endothelial cells depends on epidermal growth factor receptor tyrosine kinase activity. [Abstract]. FASEB J. Vol. 1178, no. 22: 21 (Published)
• Barson R, H Cui, Y Jin, Y Liu and LD Nelin. 2008. Hypoxia decreases nitric oxide production by lipopolysaccharide stimulated macrophages [Abstract]. Free Radic Biol Med. Vol. 45: S135 (Published)
• Strauch K, Y Jin, H Cui, B Chen and LD Nelin. 2008. Asymmetric dimethyl arginine does not inhibit arginase activity and is pro-proliferative in pulmonary endothelial cells [Abstract]. Free Radic Biol Med. Vol. 45: S48 (Published)
• Cua CL, LK Rogers, M Augustine, Y Jin, LG Chicoine and LD Nelin. 2008. Down syndrome patients with pulmonary hypertension have relatively high plasma levels of asymmetric dimethylarginine. [Abstract]. PAS. Vol. 4488: 16 (Published)
• Rogers LK, TE Tipple, LD Nelin and SE Welty. 2008. Oxidant stress in the livers of mouse pups exposed to hyperoxia [Abstract]. Free Radic Biol Med. Vol. 45: S74 (Published)
• Jin Y, LG Chicoine, Y Liu and LD Nelin. 2008. Mice deficient in MKP-1 develop severe pulmonary hypertension in hypoxia [Abstract]. PAS. Vol. 3770: 10 (Published)
• Girton MR, Y Liu, LD Nelin and LG Chicoine. 2008. MKP-1 Deficiency Augments LPS-Induced NO Production in the Lung [Abstract]. FASEB J. Vol. 1150, no. 22: 16 (Published)
• Chen B, H Cui and L Nelin. 2008. Hypoxia induces a time-dependent increase in arginase II protein expression in human pulmonary artery smooth muscle cells [Abstract]. PAS. Vol. 3770: 14 (Published)
• Calvert TJ, LG Chicoine, Y Liu and LD Nelin. 2008. Inhibition of L-arginine uptake prevents endotoxin-induced hypotension in mitogen activated protein kinase phosphatase-1 deficient mice. [Abstract]. PAS. Vol. 5620: 5 (Published)
• Stenger MR, TE Tipple, LK Rogers, Y Liu and LD Nelin. 2008. Inhaled nitric oxide prevents the hyperoxia-induced activation of p38 and JNK in neonatal mice lungs. [Abstract]. Free Radic Biiol Med. Vol. 45: S117 (Published)
• Malleske DT, Y Jin and LD Nelin. 2008. The temporal relationship of cytokine-induced expression of genes involved in the metabolism of L-arginine in bovine pulmonary artery endothelial cells [Abstract]. FASEB J. Vol. 1178, no. 22: 18 (Published)
• Tipple TE, LK Rogers, LD Nelin and SE Welty. 2008. Thioredoxin interacting protein-dependent regulation of vascular endothelial growth factor production by alveolar epithelial cells [Abstract]. Free Radic Biol Med. Vol. 45: S48 (Published)
• Stenger MR, MJ Rose, MS Joshi, LG Chicoine, JA Bauer and LD Nelin. 2007. Nitric oxide decreases protein nitration in the lungs of neonatal mice exposed to hyperoxia [Abstract]. PAS. Vol. 617740: 3 (Published)
• Tipple TE, SE Welty, LD Nelin and LK Rogers. 2007. Sublethal hyperoxia alters pulmonary thioredoxin interacting protein and vascular endothelial growth factor levels in newborn mice [Abstract]. Free Radic Biol Med. Vol. 43: S159 (Published)
• Cui H, BK English, LG Chicoine and LD Nelin. 2007. SRC family tyrosine kinases and cytokine-induced arginase expression in pulmonary microvascular endothelial cells [Abstract]. FASEB J. Vol. 21: A1431 (Published)
• Rogers LK, LD Nelin, TE Tipple and SE Welty. 2007. Administration of GW9662, a PPAR? antagonist, causes decreased inflammation and increased alveolar number in the lungs of newborn mice exposed to >95% O2 [Abstract]. Free Radic Biol Med. Vol. 43: S144 (Published)
• Stein M, T Preston, SE Welty, P Richardson, LG Chicoine and LD Nelin. 2007. Extubation improves respiratory synchrony in premature infants [Abstract]. J Perinatol. Vol. 27: 652 (Published)
• Wang X, LD Nelin, X Meng, JR Kuhlman and Y Liu. 2007. MAP kinase phosphatase (MKP)-1 plays an important role in the anti-inflammatory action of glucocorticoids [Abstract]. PAS. Vol. 618420: 3 (Published)
• Toby I, LG Chicoine and LD Nelin. 2007. EGFR stimulation promotes proliferation in human pulmonary microvascular endothelial cells [Abstract]. FASEB J. Vol. 21: A1431 (Published)
• Augustine MS, X Meng, SE Welty, LD Nelin and LK Rogers. 2007. Increases in lipid oxidation products are detectable at one day of life in newborn mice exposed to hyperoxia [Abstract]. PAS. Vol. 617931: 6 (Published)
• Cua CL, A Blankenship, AL North, J Hayes and LD Nelin. 2007. Increased incidence of idiopathic persistent pulmonary hypertension in Down syndrome neonates. Pediatric Cardiology. Vol. 28: 250-254.
• Karen L. Leonhart KL, X Meng, SE Welty, LD Nelin and LK Rogers. 2007. Plasma nitrotyrosine formation is correlated to gestational age in premature infants [Abstract]. PAS. Vol. 617935: 19 (Published)
• Evans RJ, TL Young, LG Chicoine and LD Nelin. 2007. Cytokine-induced L-arginine metabolism depends on cationic amino acid transporter activity [Abstract]. FASEB J. Vol. 21: A1430 (Published)
• Liu Y, Shepherd EG, Nelin LD. 2007. MAPK phosphatases--regulating the immune response. Nature Reviews Immunology. Vol. 7, no. 3: 202-212.
• Nankervis CA, Preston TJ, Dysart KC, Wilkinson WD, Chicoine LG, Welty SE,Nelin LD. 2007. Assessing heparin dosing in neonates on venoarterial extracorporeal membrane oxygenation. ASAIO Journal. Vol. 53, no. 1: 111-114.
• Chicoine LG, Paffett ML, Girton MR, Metropoulus MJ, Joshi MS, Bauer JA, Nelin LD, Resta TC, Walker BR. 2007. Maturational changes in the regulation of pulmonary vascular tone by nitric oxide in neonatal rats. American Journal of Physiology - Lung Cellular & Molecular Physiology. Vol. 293, no. 5: L1261-L1270.
• Wang X, Meng X, Kuhlman JR, Nelin LD, Nicol KK, English BK, Liu Y. 2007. Knockout of Mkp-1 enhances the host inflammatory responses to gram-positive bacteria. Journal of Immunology. Vol. 178, no. 8: 5312-5320.
• Nelin LD, Wang X, Zhao Q, Chicoine LG, Young TL, Hatch DM, English BK, Liu Y. 2007. MKP-1 switches arginine metabolism from nitric oxide synthase to arginase following endotoxin challenge. American Journal of Physiology - Cell Physiology. Vol. 293, no. 2: C632-C640.
• Chicoine LG, MR Girton, LD Nelin and AJ Cardounel. 2007. Developmental differences in pulmonary nitric oxide production and endogenous nitric oxide synthase inhibitors in the neonatal rat. [Abstract]. FASEB J. Vol. 21: A1440 (Published)
• Tipple TE, LK Rogers, LD Nelin and SE Welty. 2007. Sublethal hyperoxia alters hepatic but not pulmonary thioredoxin-1 levels in newborn mice [Abstract]. PAS. Vol. 618434: 4 (Published)
• Melissa J. Rose MJ, MR Stenger, MS Joshi, LD Nelin, JA Bauer. 2007. Leukocyte trafficking in hyperoxia-induced lung injury: protective effects of inhaled nitric oxide [Abstract]. PAS. Vol. 617740: 4 (Published)
• Nelin LD, DT Malleske, MR Stenger, and LG Chicoine. 2007. Vascular arginase and hypertension. Current Hypertension Review 3. Vol. 3: 242-249.
• Meng X, K Heyob, LD Nelin, SE Welty and LK Rogers. 2007. Newborn mice exposed to hyperoxia exhibit decreases in PPAR? expression [Abstract]. PAS. Vol. 617935: 7 (Published)
• Malleske, Daniel, T; Rogers, Lynette, K; Velluci, Sean, M; Young, Tamara, L; Park, Min, S; Long, Donald, W; Welty, Stephen, E; Smith, Charles, V; Nelin, Leif D. 2006. Hyperoxia increases hepatic arginase expression and ornithine production in mice. Toxicology and applied pharmacology. Vol. 215, no. 1: 109-17.
• Stenger MR, LG Chicoine, Y Liu, DM Hatch and LD Nelin. 2006. Nitric oxide increases mortality and prevents MKP-1 expression in neonatal mice exposed to hyperoxia [Abstract]. FASEB J. Vol. 20: A406 (Published)
• Wang, Xianxi; Matta, Ranyia; Shen, Gang; Nelin, Leif, D; Pei, Dehua; Liu, Yusen. 2006. Mechanism of triptolide-induced apoptosis: Effect on caspase activation and Bid cleavage and essentiality of the hydroxyl group of triptolide. Journal of molecular medicine (Berlin, Germany). Vol. 84, no. 5: 405-15.
• Zhao, Qun; Wang, Xianxi; Nelin, Leif, D; Yao, Yongxue; Matta, Ranyia; Manson, Mary, E; Baliga, Reshma, S; Meng, Xiaomei; Smith, Charles, V; Bauer, John, A; Chang, Cheong-Hee; Liu, Yusen. 2006. MAP kinase phosphatase 1 controls innate immune responses and suppresses endotoxic shock. The Journal of experimental medicine. Vol. 203, no. 1: 131-40.
• Martin EM, PJ Giannone, CA Nankervis, LG Chicoine, LJ Alexander, L Thomas, SE Welty and LD Nelin. 2006. Use of a multi-discplinary guideline-driven approach to the care of the extremely premature infant decreases length of stay [Abstract]. J Perinatol. Vol. 26: 586 (Published)
• Nelin LD, TL Young, DM Hatch, MR Stenger, LG Chicoine, BK English and Y Liu. 2006. Inhaled nitric oxide prevents the hyperoxia-induced activation of p38 and JNK in neonatal mice lungs [Abstract]. PAS. Vol. 59: 4365 (Published)
• Stenger MR, DM Hatch, LK Rogers, M Joshi, LG Chicoine, JA Bauer and LD Nelin. 2006. Nitric oxide prevents the hyperoxia-induced arrested alveolarization and decreased FGF-10 levels in neonatal mice [Abstract]. Free Radic Biol Med. Vol. 41: S54 (Published)
• Wang X, R Matta, G Shen, LD Nelin, D Pei and Y Liu. 2006. Mechanism of triptolide-induced apoptosis: effect on caspase activation and bid cleavage and essentiality of the hydroxyl group of triptolide [Abstract]. FASEB J. Vol. 20: A123 (Published)
• Rogers LK, LD Nelin and SE Welty. 2006. Newborn mice exposed to hyperoxia exhibit increases in lipid oxidation products but decreases in levels of 15-lipoxygenase and PPAR? [Abstract]. Free Radic Biol Med. Vol. 41: S151 (Published)
• Chicoine LG, MR Stenger, MJ Metroupolus, MR Girton and LD Nelin. 2006. Maturational changes in NO sensitivity are due to increases in soluble gaunylyl cyclase in the developing rat lung [Abstract]. FASEB J. Vol. 20: A400 (Published)
• Stanley, Kate, P; Chicoine, Louis, G; Young, Tamara, L; Reber, Kristina, M; Lyons, C Richard; Liu, Yusen; Nelin, Leif D. 2006. Gene transfer with inducible nitric oxide synthase decreases production of urea by arginase in pulmonary arterial endothelial cells. American journal of physiology. Lung cellular and molecular physiology. Vol. 290, no. 2: L298-306.
• Stenger MR, LG Chicoine, CV Smith, LK Rogers, SE Welty and LD Nelin. 2005. Survival and growth of neonatal mice exposed to nitric oxide and hyperoxia [Abstract]. PAS. Vol. 57: 1757 (Published)
• Chicoine Lg, E Rieger-Fackeldey, TL Young, Y Liu and LD Nelin. 2005. cytokine-induced nitric oxide production is due mainly to inducible nitric oxide synthase in bovine pulmonary arterial endothelial cells [Abstract]. PAS. Vol. 57: 837 (Published)
• Chang R, BR Walker, NL Kanagy, LG Chicoine and LD Nelin. 2005. Cytokine-induced arginase expression in bovine pulmonary arterial endothelial cells depends on Src tyrosine kinases [Abstract]. PAS. PAS ed. Vol. 57: 78 (Published)
• Zhao, Qun; Shepherd, Edward, G; Manson, Mary, E; Nelin, Leif, D; Sorokin, Andrey; Liu, Yusen. 2005. The role of mitogen-activated protein kinase phosphatase-1 in the response of alveolar macrophages to lipopolysaccharide: attenuation of proinflammatory cytokine biosynthesis via feedback control of p38. The Journal of biological chemistry. Vol. 280, no. 9: 8101-8.
• Hoffman, George, M; Nelin, Leif D. 2005. Mean airway pressure and response to inhaled nitric oxide in neonatal and pediatric patients. Lung. Vol. 183, no. 6: 441-53.
• Nelin, Leif, D; Chicoine, Louis, G; Reber, Kristina, M; English, B Keith; Young, Tamara, L; Liu, Yusen. 2005. Cytokine-induced endothelial arginase expression is dependent on epidermal growth factor receptor. American journal of respiratory cell and molecular biology. Vol. 33, no. 4: 394-401.
• Chicoine, Louis, G; Paffett, Michael, L; Young, Tamara, L; Nelin, Leif D. 2004. Arginase inhibition increases nitric oxide production in bovine pulmonary arterial endothelial cells. American journal of physiology. Lung cellular and molecular physiology. Vol. 287, no. 1: L60-8.
• Chicoine, Louis, G; Tzeng, Edith; Bryan, Rebekah; Saenz, Steven; Paffett, Michael, L; Jones, Joelle; Lyons, C Richard; Resta, Thomas, C; Nelin, Leif, D; Walker, Benjimen R. 2004. Intratracheal adenoviral-mediated delivery of iNOS decreases pulmonary vasoconstrictor responses in rats. Journal of applied physiology (Bethesda, Md. : 1985). Vol. 97, no. 5: 1814-22.
• Carter, Barney W Jr; Chicoine, Louis, G; Nelin, Leif D. 2004. L-lysine decreases nitric oxide production and increases vascular resistance in lungs isolated from lipopolysaccharide-treated neonatal pigs. Pediatric research. Vol. 55, no. 6: 979-87.
• Nelin, Leif, D; Morrisey, James, F; Effros, Richard, M; Dawson, Christopher, A; Schapira, Ralph M. 2003. The effect of inhaled nitric oxide and oxygen on the hydroxylation of salicylate in rat lungs. Pediatric research. Vol. 54, no. 3: 337-43.
• Turley, Joyce, E; Nelin, Leif, D; Kaplowitz, Mark, R; Zhang, Yongmei; Fike, Candice D. 2003. Exhaled NO is reduced at an early stage of hypoxia-induced pulmonary hypertension in newborn piglets. American journal of physiology. Lung cellular and molecular physiology. Vol. 284, no. 3: L489-500.
• Earley, Scott; Nelin, Leif, D; Chicoine, Louis, G; Walker, Benjimen R. 2002. Hypoxia-induced pulmonary endothelin-1 expression is unaltered by nitric oxide. Journal of applied physiology (Bethesda, Md. : 1985). Vol. 92, no. 3: 1152-8.
• Chicoine, Louis, G; Avitia, Jose, W; Deen, Cody; Nelin, Leif, D; Earley, Scott; Walker, Benjimen R. 2002. Developmental differences in pulmonary eNOS expression in response to chronic hypoxia in the rat. Journal of applied physiology (Bethesda, Md. : 1985). Vol. 93, no. 1: 311-8.
• Nelin, Leif, D; Krenz, Gary, S; Chicoine, Louis, G; Dawson, Christopher, A; Schapira, Ralph M. 2002. L-Arginine uptake and metabolism following in vivo silica exposure in rat lungs. American journal of respiratory cell and molecular biology. Vol. 26, no. 3: 348-55.
• Nelin, L, D; Hoffman, G M. 2001. L-arginine infusion lowers blood pressure in children. The Journal of pediatrics. Vol. 139, no. 5: 747-9.
• Nelin, L, D; Nash, H, E; Chicoine, L G. 2001. Cytokine treatment increases arginine metabolism and uptake in bovine pulmonary arterial endothelial cells. American journal of physiology. Lung cellular and molecular physiology. Vol. 281, no. 5: L1232-9.
• Guarin, M; Dawson, C, A; Nelin, L D. 2001. The arterial site of action of nitric oxide in the neonatal pig lung determined by microfocal angiography. Lung. Vol. 179, no. 1: 43-55.
• Kanagy, N, L; Walker, B, R; Nelin, L D. 2001. Role of endothelin in intermittent hypoxia-induced hypertension. Hypertension. Vol. 37, no. 2 Part 2: 511-5.
• Fike, C, D; Kaplowitz, M, R; Rehorst-Paea, L, A; Nelin, L D. 2000. L-Arginine increases nitric oxide production in isolated lungs of chronically hypoxic newborn pigs. Journal of applied physiology (Bethesda, Md. : 1985). Vol. 88, no. 5: 1797-803.
• Bousamra, M 2nd; Rossi, R; Jacobs, E; Parviz, M; Busch, C; Nelin, L, D; Haworth, S; Dawson, C A. 2000. Systemic lobar shunting induces advanced pulmonary vasculopathy. The Journal of thoracic and cardiovascular surgery. Vol. 120, no. 1: 88-98.
• Walker, B, R; Resta, T, C; Nelin, L D. 2000. Nitric oxide-dependent pulmonary vasodilation in polycythemic rats. American journal of physiology. Heart and circulatory physiology. Vol. 279, no. 5: H2382-9.
• Parviz, M; Bousamra, M 2nd; Chammas, J, H; Birks, E, K; Presberg, K, W; Jacobs, E, R; Nelin, L D. 1999. Effects of chronic pulmonary overcirculation on pulmonary vasomotor tone. The Annals of thoracic surgery. Vol. 67, no. 2: 522-7.
• Gordon, J, B; Rehorst-Paea, L, A; Hoffman, G, M; Nelin, L D. 1999. Pulmonary vascular responses during acute and sustained respiratory alkalosis or acidosis in intact newborn piglets. Pediatric research. Vol. 46, no. 6: 735-41.
• Nelin, L, D; Hoffman, G M. 1998. The use of inhaled nitric oxide in a wide variety of clinical problems. Pediatric clinics of North America. Vol. 45, no. 3: 531-48.
• Fike, C, D; Kaplowitz, M, R; Thomas, C, J; Nelin, L D. 1998. Chronic hypoxia decreases nitric oxide production and endothelial nitric oxide synthase in newborn pig lungs. The American journal of physiology. Vol. 274, no. 4 Pt 1: L517-26.
• Schapira, R, M; Wiessner, J, H; Morrisey, J, F; Almagro, U, A; Nelin, L D. 1998. L-arginine uptake and metabolism by lung macrophages and neutrophils following intratracheal instillation of silica in vivo. American journal of respiratory cell and molecular biology. Vol. 19, no. 2: 308-15.
• Nelin, L, D; Welty, S, E; Morrisey, J, F; Gotuaco, C; Dawson, C A. 1998. Nitric oxide increases the survival of rats with a high oxygen exposure. Pediatric research. Vol. 43, no. 6: 727-32.
• De La Fuente, E, K; Dawson, C, A; Nelin, L, D; Bongard, R, D; McAuliffe, T, L; Merker, M P. 1997. Biotinylation of membrane proteins accessible via the pulmonary circulation in normal and hyperoxic rats. The American journal of physiology. Vol. 272, no. 3 Pt 1: L461-70.
• Hoffman, G, M; Ross, G, A; Day, S, E; Rice, T, B; Nelin, L D. 1997. Inhaled nitric oxide reduces the utilization of extracorporeal membrane oxygenation in persistent pulmonary hypertension of the newborn. Critical care medicine. Vol. 25, no. 2: 352-9.
• Nelin, L, D; Thomas, C, J; Dawson, C A. 1996. Effect of hypoxia on nitric oxide production in neonatal pig lung. The American journal of physiology. Vol. 271, no. 1 Pt 2: H8-14.
• Nelin, L, D; Christman, N, T; Morrisey, J, F; Dawson, C A. 1996. Electrochemical nitric oxide and nitrogen dioxide analyzer for use with inhaled nitric oxide. Journal of applied physiology (Bethesda, Md. : 1985). Vol. 81, no. 3: 1423-9.
• Heimler, R; Nelin, L, D; Billman, D, O; Sasidharan, P. 1995. Identification of sepsis in neonates following maternal antibiotic therapy. Clinical pediatrics. Vol. 34, no. 3: 133-7.
• Fakler, C, R; Kaftan, H, A; Nelin, L D. 1995. Two cases suggesting a role for the L-arginine nitric oxide pathway in neonatal blood pressure regulation. Acta paediatrica (Oslo, Norway : 1992). Vol. 84, no. 4: 460-2.
• Nelin, L, D; Rickaby, D, A; Linehan, J, H; Dawson, C A. 1994. The vascular site of action of hypoxia in the neonatal pig lung. Pediatric research. Vol. 35, no. 1: 25-9.
• Nelin, L, D; Moshin, J; Thomas, C, J; Sasidharan, P; Dawson, C A. 1994. The effect of inhaled nitric oxide on the pulmonary circulation of the neonatal pig. Pediatric research. Vol. 35, no. 1: 20-4.
• Nelin, L, D; Dawson, C A. 1993. The effect of N omega-nitro-L-arginine methylester on hypoxic vasoconstriction in the neonatal pig lung. Pediatric research. Vol. 34, no. 3: 349-53.
• Nelin, L, D; Krenz, G, S; Rickaby, D, A; Linehan, J, H; Dawson, C A. 1993. A distensible vessel model applied to hypoxic pulmonary vasoconstriction in the neonatal pig. Journal of applied physiology (Bethesda, Md. : 1985). Vol. 74, no. 5: 2049-56.
• Heimler, R; Doumas, B, T; Jendrzejczak, B, M; Nemeth, P, B; Hoffman, R, G; Nelin, L D. 1993. Relationship between nutrition, weight change, and fluid compartments in preterm infants during the first week of life. The Journal of pediatrics. Vol. 122, no. 1: 110-4.
• Nelin, L, D; Wearden, M, E; Welty, S, E; Hansen, T N. 1992. The effect of blood flow and left atrial pressure on the DLCO in lambs and sheep. Respiration physiology. Vol. 88, no. 3: 333-42.
• Nelin, L, D; Roerig, D, L; Rickaby, D, A; Linehan, J, H; Dawson, C A. 1992. Influence of flow on pulmonary vascular surface area inferred from blue dextran efflux data. Journal of applied physiology (Bethesda, Md. : 1985). Vol. 72, no. 3: 874-80.
• Linehan, J, H; Haworth, S, T; Nelin, L, D; Krenz, G, S; Dawson, C A. 1992. A simple distensible vessel model for interpreting pulmonary vascular pressure-flow curves. Journal of applied physiology (Bethesda, Md. : 1985). Vol. 73, no. 3: 987-94.
• Heimler, R; Langlois, J; Hodel, D, J; Nelin, L, D; Sasidharan, P. 1992. Effect of positioning on the breathing pattern of preterm infants. Archives of disease in childhood. Vol. 67, no. 3: 312-4.
• Dawson, C, A; Roerig, D, L; Rickaby, D, A; Nelin, L, D; Linehan, J, H; Krenz, G S. 1992. Use of diazepam for interpreting changes in extravascular lung water. Journal of applied physiology (Bethesda, Md. : 1985). Vol. 72, no. 2: 686-93.
• Nelin, L, D; Rickaby, D, A; Linehan, J, H; Dawson, C A. 1991. Effect of atelectasis and surface tension on pulmonary vascular compliance. Journal of applied physiology (Bethesda, Md. : 1985). Vol. 70, no. 6: 2401-9.
• Leif Nelin, Lecturer. 1999. Inhaled Nitric Oxide and PPHN. Presented at New Mexico Association of Neonatal Nurses. Albuquerque, NM, USA.
• Leif Nelin, Chair. 2004. Neonatal Lung Injury. Presented at Society for Pediatric Research. San Francisco, CA, USA. (May) [Peer Reviewed]
• Leif Nelin, Chair. 2000. Control of Pulmonary Vascular Tone. Presented at Society for Pediatric Research. Boston, MA, USA. (May) [Peer Reviewed]
• Leif Nelin, Chair. 2009. PPHN: Mechanisms to Therapeutic Intervention. Presented at Society for Pediatric Research. Baltimore, MD, USA. (May) [Peer Reviewed]
• Leif Nelin, Chair. 2005. Vascular Mediators in Persistent Pulmonary Hypertension. Presented at Society for Pediatric Research. Washington, DC, USA. (May) [Peer Reviewed]
• Leif Nelin, Lecturer. 2008. Management of the Chronically Ventilated Premature Infant. Presented at UK Hot Topics. Berystede Hotel. Sunninghill, Ascot, UK.
• Leif Nelin, Lecturer. 2005. Neonatal Pulmonary Hypertension. Presented at Pediatric Grand Rounds. Vanderbilt University. Nashville, TN, USA.
• Leif Nelin, Lecturer. 2009. Arginine metabolism and transport in pulmonary hypertension. Presented at 11th International Congress on Amino Acids, Peptides and Proteins. University of Vienna. Vienna, Austria.
• Leif Nelin, Chair. 1996. Regulation of pulmonary vascular tone and reactivity. Presented at Experimental Biology ?96. Washington, DC, USA. (April) [Peer Reviewed]
• Leif Nelin, Lecturer. 1997. Inhaled Nitric Oxide in Pediatrics. Presented at Pediatric Infant Ventilator Conference. Milwaukee, WI, USA.
• Leif Nelin, Lecturer. 2007. Approaches to Late Ventilator Care in Severe BPD. Presented at Pediatric Academic Socities, Topic Symposium. Toronto, Canada. [Peer Reviewed]
• Leif Nelin, Lecturer. 2007. Neonatal Pulmonary Hypertension. Presented at Pediatric Grand Rounds, LeBonheur Children?s Hospital/University of Tennessee ? Memphis. LeBonheur Children?s Hospital/University of Tennessee ? Memphis. Memphis, TN, USA.
• Leif Nelin, Chair. 2008. Pulmonary Vascular Biology ? Animal Models of Pathophysiology & Treatment. Presented at Society for Pediatric Research. Honolulu, HI, USA. (May) [Peer Reviewed]
• Leif Nelin, Lecturer. 2006. Neonatal Pulmonary Hypertension. Presented at Pediatric Grand Rounds at Texas A&M University. Department of Pediatrics, Texas A&M University. Temple, TX, USA.
• Leif Nelin, Chair. 2008. Pulmonary Hypertension ? Bench to Bedside. Presented at Society for Pediatric Research. Honolulu, HI, USA. (May) [Peer Reviewed]
• Leif Nelin, Lecturer. 2005. Cell signaling and L-arginine metabolism. Presented at Neonatal Research Conference. Vanderbilt University. Nashville, TN, USA.
• Leif Nelin, Lecturer. 1994. Inhaled nitric oxide. Presented at Acute Care Pediatrics: Review and Update of the State of the Art. Hilton Head, SC, USA.
• Leif Nelin, Chair. 2006. Oxygen and Nitric Oxide: A Marriage Made in Heaven or Hell? Presented at Society for Pediatric Research. San Francisco, CA, USA. (May) [Peer Reviewed]

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