Amit Kapoor, PhD :: Nationwide Children's Hospital, Columbus, Ohio

Amit Kapoor, PhD

Amit Kapoor, PhD

Center for Vaccines and Immunity
Principal Investigator

Contact Information

700 Children's Drive
WA4015
Columbus, OH 43205 [ map ]
PH: (614) 722-2735
FX: (614) 722-3680
Email Me

Biography

In 2015, Amit Kapoor, PhD, joined The Center for Vaccines & Immunity at The Research Institute at Nationwide Children’s Hospital as a principal investigator and The Ohio State University Department of Pediatrics as an Associate Professor. Dr. Kapoor’s work focuses on molecular characterization of polio and enteroviruses associated with acute flaccid paralysis in North Indian children. This work was part of an effort to eradicate wild polioviruses from India using vaccination and surveillance. Dr. Kapoor joined Dr. Eric Delwart (UCSF) in 2002 as post doc and helped in the identification of several new human and animal viruses, including human cosavirus, bocaviruses, astroviruses, parvoviruses and picornaviruses. Dr. Kapoor joined Columbia University as Assistant professor in 2010, where he identified more new human and animal viruses including the first non- human primate homologs of Hepatitis C virus. His most recent work was the identification of human hepegivirus (also known as Human Pegivirus 2) in blood transfusion recipients and the development of VirCapSeq (a pan virome sequencing enrichment method, similar to the exon capture method).

Gender:

  • Male

Languages Spoken:

  • English

Research Interests

Research Center:

Areas of Interest:

  • Role of virome dynamics in human health and diseases
  • Ongoing research projects involves virus characterization using in vitro and in vivo infections, mutagenesis, serology and high throughout sequencing for new virus identification, virome analysis and RNAseq. Dr Kapoor’s lab team and software programmer work in concert for meaningful analysis of the sequencing data. The work is supported by The Research Institute’s vivarium, biomedical genomics core (HiSeq4000) and high performance computing cluster (992 cores, 11.5TB memory, 37.4 TFLOPs).
  • Identification of emerging human and animal viruses
  • Dr. Kapoor identified the first non-primate homologs of Hepatitis C virus, including the well-known equine and rodent hepaciviruses. Absent immune-competent animal models for HCV, homolog hepaciviruses in their natural hosts could provide useful surrogate models to understand HCV pathogenesis and immunity. Currently, Dr Kapoor’s lab is characterizing the biological properties of these new HCV-like animal viruses using in vitro and reverse genetic systems paving the way for their detailed in vivo characterization. His main focus is to use these surrogate models to identify the determinants of HCV persistence and protective immunity.
  • Studies of HCV pathogenesis and immunity using surrogate animal hepaciviruses
  • Research in Dr. Kapoor’s lab is focused on understanding the health relevance of virome dynamics and development of surrogate models for human viruses. Studies of viruses in their natural hosts are necessary to gain explicit insights into the mechanisms of virus evolution, pathogenesis and immunity. His lab is using state-of-the-science techniques to find new viruses and to study health relevance of virome dynamics using transcriptome analysis (RNA-seq). Dr Kapoor’s lab is utilizing longitudinal study design to gain comprehensive understanding of changes that presage development of acute and chronic diseases.

Research Funding*:

  • USDA/NIH
    2014/05/05 - 2019/03/31
    Rice, Charles M. (PI)
    Characterization of recently discovered liver-tropic viruses in horses
  • NIH 7 R21 AI 107631-03
    2015/11/01 – 2016/06/30
    Kapoor, Amit (PI)
    Study of rodent HCV-GBV like viruses
    National Institute of Allergy and Infectious Diseases (NIAID)
  • • R01 HL119485-01A1
    2014/05/05 - 2019/03/31
    National Heart, Lung and Blood Institute (NHLBI)
    Kapoor, Amit (PI)
    Novel viruses and viral dynamics in multiple transfusion recipients
  • National Science Foundation/Ecology and Evolution of Infectious Diseases Program
    Vandegrift, Kurt (PI)
    Interacting viromes of white-footed mice and blacklegged ticks: virome assembly and transmission in human-dominated landscapes

Education and Training

Fellowship

  • Sanjay Gandhi Post Graduate Institute of Medical Sciences
    Date Completed: 12/31/2002

Doctorate

  • Sanjay Gandhi Post Graduate Institute of Medical Sciences
    Date Completed: 12/31/2003

Postdoctoral Training

  • University of California, Blood Systems Research Institute
    Date Completed: 12/31/2006

Department:

  • Center for Vaccines and Immunity

Date of Appointment at Nationwide Children’s Hospital:

  • 10/26/2015

Professional Experience

2015–present

  • Associate Professor, Department of Pediatrics, The Ohio State University

2015–present

  • Principal Investigator, Vaccines & Immunity Center, The Research Institute at Nationwide Children's Hospital

2010–2015

  • Assistant Professor, Pathology & Cell Biology, Columbia University, New York, NY

2010–2010

  • Associate Research Scientist, Infection & Immunity Center, Columbia University, New York, NY

2006–2009

  • Scientist, Molecular Virology, Blood Systems Research Institute, University of California, San Francisco, CA

Publications

  • Kapoor A1, Simmonds P, Scheel TK, Hjelle B, Cullen JM, Burbelo PD, Chauhan LV, Duraisamy R, Sanchez Leon M, Jain K, Vandegrift KJ, Calisher CH, Rice CM, Lipkin WI. Identification of rodent homologs of hepatitis C virus and pegiviruses. MBio. 2013 Apr 9;4(2):e00216-13. doi: 10.1128/mBio.00216-13. PubMed ID: 23572554
  • Burbelo PD1, Ching KH, Esper F, Iadarola MJ, Delwart E, Lipkin WI, Kapoor A. Serological studies confirm the novel astrovirus HMOAstV-C as a highly prevalent human infectious agent. PLoS One. 2011;6(8):e22576. doi: 10.1371/journal.pone.0022576. Epub 2011 Aug 4. PubMed ID: 21829634
  • Kapoor A1, Simmonds P, Lipkin WI. Discovery and characterization of mammalian endogenous parvoviruses. J Virol. 2010 Dec;84(24):12628-35. doi: 10.1128/JVI.01732-10. Epub 2010 Oct 13. PubMed ID: 20943964
  • Burbelo PD1, Dubovi EJ, Simmonds P, Medina JL, Henriquez JA, Mishra N, Wagner J, Tokarz R, Cullen JM, Iadarola MJ, Rice CM, Lipkin WI, Kapoor A. Serology-enabled discovery of genetically diverse hepaciviruses in a new host. J Virol. 2012 Jun;86(11):6171-8. doi: 10.1128/JVI.00250-12. Epub 2012 Apr 4. PubMed ID: 22491452
  • Burbelo PD1, Dubovi EJ, Simmonds P, Medina JL, Henriquez JA, Mishra N, Wagner J, Tokarz R, Cullen JM, Iadarola MJ, Rice CM, Lipkin WI, Kapoor A. Serology-enabled discovery of genetically diverse hepaciviruses in a new host. J Virol. 2012 Jun;86(11):6171-8. doi: 10.1128/JVI.00250-12. Epub 2012 Apr 4. PubMed ID: 22491452
  • Kapoor A1, Victoria J, Simmonds P, Slikas E, Chieochansin T, Naeem A, Shaukat S, Sharif S, Alam MM, Angez M, Wang C, Shafer RW, Zaidi S, Delwart E. A highly prevalent and genetically diversified Picornaviridae genus in South Asian children. Proc Natl Acad Sci U S A. 2008 Dec 23;105(51):20482-7. doi: 10.1073/pnas.0807979105. Epub 2008 Nov 25. PubMed ID: 19033469
  • Alagaili AN, Briese T, Mishra N, Kapoor V, Sameroff SC, Burbelo PD, de Wit E, Munster VJ, Hensley LE, Zalmout IS, Kapoor A, Epstein JH, Karesh WB, Daszak P, Mohammed OB, Lipkin WI. Middle East respiratory syndrome coronavirus infection in dromedary camels in Saudi Arabia. MBio. 2014 Feb 25;5(2):e00884-14. doi: 10.1128/mBio.00884-14. PubMed ID: 24570370
  • Kapoor A1, Simmonds P, Cullen JM, Scheel TK, Medina JL, Giannitti F, Nishiuchi E, Brock KV, Burbelo PD, Rice CM, Lipkin WI. Identification of a pegivirus (GB virus-like virus) that infects horses. J Virol. 2013 Jun;87(12):7185-90. doi: 10.1128/JVI.00324-13. Epub 2013 Apr 17. PubMed ID: 23596285
  • Scheel TK1, Simmonds P2, Kapoor A3. Surveying the global virome: identification and characterization of HCV-related animal hepaciviruses. Antiviral Res. 2015 Mar;115:83-93. doi: 10.1016/j.antiviral.2014.12.014. Epub 2014 Dec 26. PubMed ID: 25545071
  • Kapoor A1, Kumar A2, Simmonds P3, Bhuva N2, Singh Chauhan L2, Lee B2, Sall AA4, Jin Z5, Morse SS6, Shaz B7, Burbelo PD8, Lipkin WI2. Virome Analysis of Transfusion Recipients Reveals a Novel Human Virus That Shares Genomic Features with Hepaciviruses and Pegiviruses. MBio. 2015 Sep 22;6(5):e01466-15. doi: 10.1128/mBio.01466-15. PubMed ID: 26396247
  • Kapoor A1, Dubovi EJ, Henriquez-Rivera JA, Lipkin WI. Complete genome sequence of the first canine circovirus. J Virol. 2012 Jun;86(12):7018. doi: 10.1128/JVI.00791-12. PubMed ID: 22628401
  • Briese T1, Kapoor A2, Mishra N3, Jain K3, Kumar A3, Jabado OJ3, Lipkin WI4. Virome Capture Sequencing Enables Sensitive Viral Diagnosis and Comprehensive Virome Analysis. MBio. 2015 Sep 22;6(5):e01491-15. doi: 10.1128/mBio.01491-15. PubMed ID: 26396248
  • Victoria JG1, Kapoor A, Dupuis K, Schnurr DP, Delwart EL. Rapid identification of known and new RNA viruses from animal tissues. PLoS Pathog. 2008 Sep 26;4(9):e1000163. doi: 10.1371/journal.ppat.1000163. PubMed ID: 18818738
  • Burbelo PD1, Ragheb JA, Kapoor A, Zhang Y. The serological evidence in humans supports a negligible risk of zoonotic infection from porcine circovirus type 2. Biologicals. 2013 Nov;41(6):430-4. doi: 10.1016/j.biologicals.2013.09.005. Epub 2013 Oct 10. PubMed ID: 24120888
  • Kapoor A1, Simmonds P, Slikas E, Li L, Bodhidatta L, Sethabutr O, Triki H, Bahri O, Oderinde BS, Baba MM, Bukbuk DN, Besser J, Bartkus J, Delwart E. Human bocaviruses are highly diverse, dispersed, recombination prone, and prevalent in enteric infections. J Infect Dis. 2010 Jun 1;201(11):1633-43. doi: 10.1086/652416. PubMed ID: 20415538
  • Kapoor A1, Tesh RB, Duraisamy R, Popov VL, Travassos da Rosa AP, Lipkin WI. A novel mosquito-borne Orbivirus species found in South-east Asia. J Gen Virol. 2013 May;94(Pt 5):1051-7. doi: 10.1099/vir.0.046748-0. Epub 2013 Jan 30. PubMed ID: 23364187
  • Kapoor A1, Dubovi EJ, Henriquez-Rivera JA, Lipkin WI. Complete genome sequence of the first canine circovirus. J Virol. 2012 Jun;86(12):7018. doi: 10.1128/JVI.00791-12. PubMed ID: 22628401

Awards, Honors and Organizations

  • One of two PNAS papers selected for the section “In this issue” and both articles were accompanied by commentaries
  • Work highlighted by New York Times and other science media sites
  • Guest appearance, twice, on “This week in virology” (TWiV.tv) – a widely popular science podcast
  • Journal of Virology paper selected for Faculty of 1000 Biology
  • Two Journal of Virology papers selected for “Spotlight” section
  • Work featured as cover page of The Journal of Infectious diseases and the ASM Journal mBio
Nationwide Children's Hospital
700 Children's Drive Columbus, Ohio 43205 614.722.2000