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Laboratory Test Directory

Targeted Tumor Variant Analysis

Overview
Specimen Requirements
Billing Code

Components

Name Method Department Units
Targeted Tumor Variant Analysis DNA extraction
Polymerase chain reaction (PCR)
Sanger sequencing
Molecular Genetics
Test Code

TTVA

Typical Turnaround Time

10 days

Setup Schedule

All tests not performed daily.
Monday through Friday

Specimen Requirements

Bone marrow, Whole blood

Container Type Container Size Specimen Volume
Preferred

Purple tube (EDTA)

 4 mL 4 mL
Stability

Room temperature - 24 hour(s)
Refrigerated - 72 hour(s)

Specimen Preparation

  • Do not freeze
  • Keep at room temperature or refrigerate
  • Do not centrifuge
  • Tumor sample must contain a minimum of 50% tumor

Tissue (Fresh)

Container Type Container Size Specimen Volume
Preferred

Tissue culture transport media

 N/A 5mm x 5mm or larger
Alternate

Sterile container with saline

 N/A 5mm x 5mm or larger
Stability

Room temperature - 24 hour(s)
Refrigerated - 48 hour(s)

Specimen Preparation

  • Do not add fixative
  • Do not freeze
  • Keep at room temperature or refrigerate
  • Transport to laboratory as soon as possible
  • Tumor sample must contain a minimum of 40% tumor

Paraffin embedded tissue

Container Type Container Size Specimen Volume
Preferred

Paraffin block

 N/A
Stability

Room temperature - 6 month(s)

Specimen Preparation

  • Protect from heat
  • Keep at room temperature
  • Tumor sample must contain a minimum of 40% tumor

Tissue (Snap-frozen)

Container Type Container Size Specimen Volume
Preferred

Tissue cassette

 N/A 5mm x 5mm or larger
Alternate

Cryogenic tube

 N/A 5mm x 5mm or larger
Stability

Frozen - 12 month(s)

Specimen Preparation

  • Keep frozen
  • Protect from heat
  • Transport to laboratory as soon as possible
  • Immediately place on dry ice and transport frozen
  • Tumor sample must contain a minimum of 40% tumor

Tissue scrolls (FFPE)

Container Type Container Size Specimen Volume
Preferred

Sterile container

 N/A 10 scrolls
Stability

Room temperature - 6 month(s)

Specimen Preparation

  • Keep at room temperature
  • Protect from heat
  • Must accompany H&E slide from the same tissue block used to make FFPE scrolls
  • Tumor sample must contain a minimum of 40% tumor

Tissue scrolls (OCT)

Container Type Container Size Specimen Volume
Preferred

Cryogenic tube

 N/A 10 scrolls
Stability

Frozen - 12 month(s)

Specimen Preparation

  • Keep frozen
  • Protect from heat
  • Immediately place on dry ice and transport frozen
  • Transport to laboratory as soon as possible
  • Tumor sample must contain a minimum of 40% tumor
  • Must accompany H&E slide from the same OCT-embedded tissue used to make OCT tissue scrolls

OCT-embedded tissue

Container Type Container Size Specimen Volume
Preferred

Tissue cassette

 N/A 5mm x 5mm or larger
Alternate

Cryogenic tube

 N/A 5mm x 5mm or larger
Stability

Frozen - 12 month(s)

Specimen Preparation

  • Keep frozen
  • Protect from heat
  • Immediately place on dry ice and transport frozen
  • Transport to laboratory as soon as possible
  • Tumor sample must contain a minimum of 40% tumor

Reasons for Rejection

  • Inadequate tissue
  • Tissue degradation
  • Delayed or improper handling

Comments

Please click on the Lab Form Link in the Forms Section to print and complete the Pediatric Oncology Test Requisition Form. Submission of completed Genetic Test Requisition Form is required, and submission of informed consent form is recommended.

This test is a targeted Sanger sequencing analysis used to confirm the presence of a specific DNA sequence change in tumor sample that was previously identified by a research testing or by another clinical laboratory. Please submit a copy of the previous tumor sequencing result, if available. For liquid tumor, sample must contain at least 50% blasts by internal pathology review. For solid tumor, samle must contain at least 40% tumor by internal pathology review. Submission of a normal sample (containing 0% tumor) is recommended to rule out germline mutation, but not required. Please send frozen samples (e.g. snap-frozen tissue or OCT-embedded tissue) buried in adequate amount of dry ice and ship by overnight courier.

For questions, please call (614) 722-2866.

Forms

Lab Form

CPT Code

  • 81479