Laboratory Test Directory
Targeted Tumor Variant Analysis
Components
Name | Method | Department | Units |
---|---|---|---|
Targeted Tumor Variant Analysis |
DNA extraction Polymerase chain reaction (PCR) Sanger sequencing |
Molecular Genetics |
Specimen Requirements
Bone marrow, Whole blood
Container Type | Container Size | Specimen Volume |
---|---|---|
PreferredPurple tube (EDTA) |
4 mL | 4 mL |
Stability
Room temperature - 24 hour(s) |
||
Specimen Preparation
- Do not freeze
- Keep at room temperature or refrigerate
- Do not centrifuge
- Tumor sample must contain a minimum of 50% tumor
Tissue (Fresh)
Container Type | Container Size | Specimen Volume |
---|---|---|
PreferredTissue culture transport media |
N/A | 5mm x 5mm or larger |
AlternateSterile container with saline |
N/A | 5mm x 5mm or larger |
Stability
Room temperature - 24 hour(s)
Refrigerated - 48 hour(s)
Specimen Preparation
- Do not add fixative
- Do not freeze
- Keep at room temperature or refrigerate
- Transport to laboratory as soon as possible
- Tumor sample must contain a minimum of 40% tumor
Paraffin embedded tissue
Container Type | Container Size | Specimen Volume |
---|---|---|
PreferredParaffin block |
N/A | |
Stability
Room temperature - 6 month(s)
Specimen Preparation
- Protect from heat
- Keep at room temperature
- Tumor sample must contain a minimum of 40% tumor
Tissue (Snap-frozen)
Container Type | Container Size | Specimen Volume |
---|---|---|
PreferredTissue cassette |
N/A | 5mm x 5mm or larger |
AlternateCryogenic tube |
N/A | 5mm x 5mm or larger |
Stability
Frozen - 12 month(s)
Specimen Preparation
- Keep frozen
- Protect from heat
- Transport to laboratory as soon as possible
- Immediately place on dry ice and transport frozen
- Tumor sample must contain a minimum of 40% tumor
Tissue scrolls (FFPE)
Container Type | Container Size | Specimen Volume |
---|---|---|
PreferredSterile container |
N/A | 10 scrolls |
Stability
Room temperature - 6 month(s)
Specimen Preparation
- Keep at room temperature
- Protect from heat
- Must accompany H&E slide from the same tissue block used to make FFPE scrolls
- Tumor sample must contain a minimum of 40% tumor
Tissue scrolls (OCT)
Container Type | Container Size | Specimen Volume |
---|---|---|
PreferredCryogenic tube |
N/A | 10 scrolls |
Stability
Frozen - 12 month(s)
Specimen Preparation
- Keep frozen
- Protect from heat
- Immediately place on dry ice and transport frozen
- Transport to laboratory as soon as possible
- Tumor sample must contain a minimum of 40% tumor
- Must accompany H&E slide from the same OCT-embedded tissue used to make OCT tissue scrolls
OCT-embedded tissue
Container Type | Container Size | Specimen Volume |
---|---|---|
PreferredTissue cassette |
N/A | 5mm x 5mm or larger |
AlternateCryogenic tube |
N/A | 5mm x 5mm or larger |
Stability
Frozen - 12 month(s)
Specimen Preparation
- Keep frozen
- Protect from heat
- Immediately place on dry ice and transport frozen
- Transport to laboratory as soon as possible
- Tumor sample must contain a minimum of 40% tumor
Reasons for Rejection
- Inadequate tissue
- Tissue degradation
- Delayed or improper handling
- Wrong type of specimen
Comments
Please click on the Lab Form Link in the Forms Section to print and complete the Pediatric Oncology Test Requisition Form. Submission of completed Genetic Test Requisition Form is required, and submission of informed consent form is recommended but not required.
This test is a targeted Sanger sequencing analysis used to confirm the presence of a specific DNA sequence change in tumor sample that was previously identified by a research testing or by another clinical laboratory. This test is only available as a CONFIRMATORY TEST and only offered to patients who have a known somatic sequence variant(s) detected by previous research or clinical testing. If patient does not have a previously detected somatic variant(s), then this test cannot be ordered and samples will be rejected.
Please submit a copy of the previous tumor sequencing result, if available. For liquid tumor, sample must contain at least 50% blasts by internal pathology review. For solid tumor, samle must contain at least 40% tumor by internal pathology review. Submission of a normal sample (containing 0% tumor) is recommended to rule out germline mutation, but not required. Please send frozen samples (e.g. snap-frozen tissue or OCT-embedded tissue) buried in adequate amount of dry ice and ship by overnight courier.
For questions, please call (614) 722-2866.
Forms
CPT Code
- 81479