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Laboratory Test Directory

System Test (Updated)

Components

Name Method Department Units
System Test Bioassay
High-pressure liquid chromatography/tandem mass spectrometry (HPLC/MS-MS), equilibrium dialysis
Becki Tests GPL; MPL - IgG and IgM Phospholipid antibody units

Battery Members

Test, % Test, System Test, Display Test

Specimen Requirements

Urine

Container Type Container Size Specimen Volume
Preferred

Sterile container

1.5 mL na
Stability

Refrigerated - NA hour(s)

Specimen Preparation

  • Transport to laboratory as soon as possible

Whole blood

Container Type Container Size Specimen Volume
Preferred

Green tube (Lithium heparin), No Gel

3 mL 3 mL
Alternate

Green tube (Lithium heparin), No Gel

Micro sample tube 1 mL
Alternate

Red tube, (no anticoagulant), No Gel

3 mL 3 mL
Alternate

Gold tube, (SST) with Gel

3.5 mL 3 mL
Alternate

Purple tube (EDTA)

3 mL 3 mL
Stability

Room temperature - 24 hour(s)
Refrigerated - 48 hour(s)
Frozen - 72 hour(s)

Specimen Preparation

  • Keep refrigerated

Serum Spun, Plasma Spun

Container Type Container Size Specimen Volume
Preferred

Green tube (Lithium heparin), No Gel

3 mL 3 mL
Alternate

Green tube (Lithium heparin), No Gel

Micro sample tube 1 mL
Alternate

Red tube, (no anticoagulant), No Gel

3 mL 3 mL
Alternate

Gold tube, (SST) with Gel

3.5 mL 3 mL
Stability

Room temperature - 36 hour(s)
Refrigerated - 72 hour(s)
Frozen - 72 hour(s)

Specimen Preparation

  • Centrifuge within 4 hours, separate supernatant and freeze

Throat swab

Container Type Container Size Specimen Volume
Preferred

Dual culture swab system

N/A
Stability

Room temperature - 24 hour(s)
Refrigerated - 48 hour(s)

Specimen Preparation

  • Do not freeze

Reasons for Rejection

  • Air in specimen
  • Exposure to air

Comments

A single blood collection is considered one draw dispensed into one Aerobic (Green)and one Anaerobic (Purple) bottle. The amount of blood collected depends on the weight of the patient following hospital guidelines:

If <1 mL collected, place entire sample in Aerobic (Green) bottle. Bone Marrow: Inoculate entire sample into Aerobic (Green) bottle. Blood culture bottles are incubated 5 days before being finalized. Positive blood culture results are called to physician immediately upon detection. A patient under the age of 4 weeks, in whom a blood culture is being ordered and thus a diagnosis of bacterial sepsis is suspected, should generally receive a full sepsis workup in the ED setting including a lumbar puncture and catheterized urine culture. If this culture is being collected at a lab draw site this recommendation will be faxed and called to the ordering physicians office.

Blood FA

The Blood Culture Identification Panel (Blood FA) is a molecular test that utilizes highly multiplexed PCR to specifically identify 24 organisms and antimicrobial resistance genes within 1-2 hours after the Gram stain reveals organisms in a blood culture bottle. This test allows the preliminary identification of potential pathogens in positive blood cultures. All Blood FA results will be confirmed by culture.

Gram-negative bacteria (11) Gram-positive bacteria (8)
Enterobacteriaceae Staphylococcus spp.
Escherichia coli Staphylococcus aureus
Enterobacter cloacae complex Streptococcus spp.
Klebsiella oxytoca Streptococcus agalactiae
Klebsiella pneumoniae Streptococcus pyogenes
Serratia marcescens Streptococcus pneumoniae
Proteus spp. Enterococcus spp.
Acinetobacter baumannii Listeria monocytogenes
Haemophilus influenzae  
Neisseria meningitidis Yeast (5)
Pseudomonas aeruginosa Candida albicans
  Candida glabrata
Antibiotic resistance Markers (2) Candida krusei
mecA (MRSA) Candida parapsilosis
vanA/vanB (VRE) Candida tropicalis

 

The Blood FA panel will be run on first time positive blood cultures only. It will NOT be run on: body fluids in blood culture media, pathology blood cultures and any bottles that come from outside hospitals

 

Clinical Reference

1. Feinstein JA, Ernst LM, Ganesh J, Feudtner C. What new information pediatric autopsies can provide: a retrospective evaluation of 100 consecutive autopsies using family-centered criteria. Archives of Pediatrics & Adolescent Medicine. 2007;161(12):1190-6.

2. Newton D, Coffin CM, Clark EB, Lowichik A. How the pediatric autopsy yields valuable information in a vertically integrated health care system. Archives of Pathology & Laboratory Medicine. 2004;128(11):1239-46.

3. Cardoso MP, Bourguignon DC, Gomes MM, Saldiva PH, Pereira CR, Troster EJ. Comparison between clinical diagnoses and autopsy findings in a pediatric intensive care unit in S?o Paulo, Brazil. Pediatric Critical Care Medicine. 2006;7(5):423-7.

Optimal test for the initial and confirmatory diagnosis of any suspected hemoglobinopathy. A faculty hematopathologist personally directs and interprets each stage of testing to completion. A comprehensive report is provided. Do not use for the followup of an individual with a known diagnosis. (Ctrl/V)

Optimal test for the initial and confirmatory diagnosis of any suspected hemoglobinopathy. A faculty hematopathologist personally directs and interprets each stage of testing to completion. A comprehensive report is provided. Do not use for the followup of an individual with a known diagnosis (word button CTRL/V)

 

Optimal test for the initial and confirmatory diagnosis of any suspected hemoglobinopathy. A faculty hematopathologist personally directs and interprets each stage of testing to completion. A comprehensive report is provided. Do not use for the followup of an individual with a known diagnosis (plain text button CTRL V)

Optimal test for the initial and confirmatory diagnosis of any suspected hemoglobinopathy. A faculty hematopathologist personally directs and interprets each stage of testing to completion. A comprehensive report is provided. Do not use for the followup of an individual with a known diagnosis (right click)

Testing recommendations: here

 

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CPT Code

  • 88305
  • 88342
  • added IHC as needed (88341 per additional antibody)