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Laboratory Test Directory

Soft Tissue Sarcoma Tumor Analysis by RT-PCR


Name Method Department Units
Soft Tissue Sarcoma Tumor Analysis by RT-PCR Reverse transcriptase polymerase chain reaction (RT-PCR)
DNA sequencing
Molecular Genetics

Specimen Requirements

Snap frozen specimen

Container Type Container Size Specimen Volume

Sterile container


Frozen - 24 hour(s)

Specimen Preparation

  • Place on ice immediately after collection
  • Transport to laboratory as soon as possible
  • Immediately place on dry ice and transport frozen
  • Do not add fixative

Reasons for Rejection

  • Delayed or improper handling
  • Tissue degradation
  • Formalin Fixed Tissue
  • Thawed frozen specimen


Please click on the Lab Form Link in the Forms Section to print and complete the Pediatric Oncology Test Requisition Form. Submission of completed Genetic Test Requisition Form is required, and submission of informed consent form is recommended. 

Testing available for the following solid tumors: Ewing sarcoma(EWS)/PNET EWS-FLI-1 and EWS-ERG fusions [t(11;22)(q24;q12) or t(21;22)(q22;q12]; Alveolar rhabdomyosarcoma (ARMS) PAX3-FOXO1 and PAX7-FOXO1 fusions [t(2;13)(q35;q14) and t(1;13)(p36;q14)]; Synovial sarcoma (SS)SYT-SSX1/SSX2 fusion [t(X;18)(p11.2;q11.2)]; Desmoplastic small round cell tumor (DSRCT) EWS-WT1 fusion [t(11;22)(p13;q12)]; and congenital fibrosarcoma/cellular mesoblastic nephroma (CMN) ETV6-NTRK3 fusion [t(12;15)(p13;q25)]. Fusion partners are confirmed by DNA sequence analysis of the RT-PCR product.

We accept snap-frozen or OCT-embedded tissue. Handle using RNA precautions and transport on dry ice. Paraffin-embedded tissue is NOT accepted for this testing.

Send any available pathology reports, even if preliminary.

Ship for overnight delivery. Saturday deliveries are accepted. Please check “Saturday Delivery” on shipment label. Please call (614) 722-2866 with questions.


Lab Form

CPT Code

  • 81401 (x4)
  • 81479 (x3)