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Laboratory Test Directory

Soft Tissue Sarcoma Tumor Analysis by RT-PCR

Components

Name Method Department Units
Soft Tissue Sarcoma Tumor Analysis by RT-PCR Reverse transcriptase polymerase chain reaction (RT-PCR)
DNA sequencing
Molecular Genetics

Specimen Requirements

Tissue (Snap-frozen)

Container Type Container Size Specimen Volume
Preferred

Tissue cassette

N/A
Alternate

Cryogenic tube

N/A
Stability

Frozen - 6 month(s)

Specimen Preparation

  • Keep frozen
  • Do not add fixative
  • Immediately place on dry ice and transport frozen
  • Transport to laboratory as soon as possible
  • Protect from heat

OCT-embedded tissue

Container Type Container Size Specimen Volume
Preferred

Tissue cassette

N/A
Alternate

Cryogenic tube

N/A
Stability

Frozen - 6 month(s)

Specimen Preparation

  • Keep frozen
  • Transport to laboratory as soon as possible
  • Protect from heat
  • Immediately place on dry ice and transport frozen

Reasons for Rejection

  • Delayed or improper handling
  • Tissue degradation
  • Formalin Fixed Tissue
  • Thawed frozen specimen
  • Wrong type of specimen

Comments

Please click on the Lab Form Link in the Forms Section to print and complete the Oncology Genetic Test Requisition Form. Submission of completed Genetic Test Requisition Form is required, and submission of informed consent form is recommended (but not requied). 

This test detects specific gene fusions associated with the following sarcomas listed below. 

  • Ewing sarcoma(EWS)/Primitive neuroectodermal tumors (PNET): EWS-FLI-1 and EWS-ERG fusions [t(11;22)(q24;q12) and t(21;22)(q22;q12]
  • Alveolar rhabdomyosarcoma (ARMS): PAX3-FOXO1 and PAX7-FOXO1 fusions [t(2;13)(q35;q14) and t(1;13)(p36;q14)]
  • Synovial sarcoma (SS): SYT-SSX1/SSX2 fusion [t(X;18)(p11.2;q11.2)]
  • Desmoplastic small round cell tumor (DSRCT): EWS-WT1 fusion [t(11;22)(p13;q12)]
  • Congenital fibrosarcoma/Cellular mesoblastic nephroma (CMN): ETV6-NTRK3 fusion [t(12;15)(p13;q25)].

Testing for one or more specific sarcoma types is available, as well as for all sarcoma types (Full Panel) -- Please specify on the Requisition Form. Fusion partners are confirmed by DNA sequence analysis of the RT-PCR product.

Only snap-frozen or OCT-embedded tissue is accepted for this testing. Paraffin-embedded tissue is NOT accepted for this testing. Handle samples using RNA precautions and transport on dry ice. Please send any available pathology reports, even if preliminary. Ship all samples frozen via overnight delivery. Saturday deliveries are accepted. Please check "Saturday Delivery" on shipment label. Please call (614) 722-2866 with questions.

Forms

Lab Form

CPT Code

  • 81401 (x4)
  • 81479 (x3)