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Laboratory Test Directory

Factor II, Quantitative


Name Method Department Units
Quantitative Factor II Clot detection
Core Lab, Coagulation %

Specimen Requirements

Platelet poor plasma

Container Type Container Size Specimen Volume

Blue plastic tube (Citrate)

2.7 mL 2.7 mL

Frozen - 2 week(s)


Blue plastic tube (Citrate)

1.8 mL 1.8 mL

Frozen - 2 week(s)

Specimen Preparation

  • Fill tubes to appropriate mark.
  • Mix by gentle inversion immediately after collection
  • Centrifuge, remove plasma/serum and freeze
  • Plasma must be platelet free

Reasons for Rejection

  • Not received within specified time frame
  • Gross hemolysis
  • Over or under filled tube
  • Clotted specimen
  • Wrong collection tube
  • Capillary specimen
  • Specimen collected in glass tube


This assay is used to quantitate coagulation Factor II. Testing for Prothrombin (Factor II) mutation is a separate assay (LIS Code: F2PCR). Please indicate on the label the collection method, e.g. line draw with heparin, line draw without heparin, venipuncture. Also indicate if the patient is receiving Coumadin (Warfarin) therapy. Centrifuge citrate tubes according to Lab policy, remove platelet-poor plasma , pool the plasma, aliquot and freeze. Send a minimum of two (2) aliquots of plasma.

CPT Code

  • 85210