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Laboratory Test Directory

Peripheral Blood Immunophenotype for Transplant Patients


Name Method Department Units
PB Transplant Immunophenotype Flow cytometry
Core Lab, Flow Cytometry %

Battery Members

CD3, CD4, CD8, CD4:CD8 ratio, CD19, CD56+ 16, CD25

Specimen Requirements

Whole blood

Container Type Container Size Specimen Volume

Purple tube (EDTA)

3 mL 3 mL

Purple tube (EDTA)

Micro sample tube 1 mL

Room temperature - 24 hour(s)

Specimen Preparation

  • Keep at room temperature
  • Mix EDTA tube by gentle inversion immediately after collection
  • Transport to laboratory as soon as possible
  • Do not refrigerate
  • Do not centrifuge purple EDTA tube

Reasons for Rejection

  • Not received within specified time frame
  • Clotted specimen
  • Specimen received on wrong day of week.
  • Refrigerated specimen
  • Centrifuged specimen


This test is used to monitor patients who have received an organ transplant, and are receiving drugs that require CD25 monitoring. To test Peripheral Blood T and B Cell Subsets on all other patients, please order Peripheral T&B Quantitation (LIS code: IPB).  Test is performed Monday - Friday but not on national holidays-plan collection accordingly. Contact Flow Cytometry Lab at 614-722-5482 with questions about the setup schedule and specimen acceptability. Specimens collected on Friday must be received by 1:00 p.m. Test is not setup on Saturday or Sunday, but specimens can be collected on Sunday after 12:00 p.m. (noon) unless Monday is a national holiday. 

CPT Code

  • 88184
  • 86355
  • 86357
  • 86359
  • 86360