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Laboratory Test Directory

Lymphocyte Subset (T, B, NK Cells) Quantitation by Flow, Blood

Components

Name Method Department Units
Lymphocyte Subset (T, B, NK Cells) Quantitation by Flow, Blood Flow cytometry
Core Lab, Diagnostic Immunology %

Battery Members

CD3, CD4, CD8, CD4:CD8 ratio, CD19+CD20+, CD56+16+, CD45+ALC, CD3+CD4-CD8-DNT cells

Specimen Requirements

Whole blood

Container Type Container Size Specimen Volume
Preferred

Purple tube (EDTA)

3 mL 3 mL
Stability

Room temperature - 48 hour(s)
Refrigerated - 48 hour(s)

Alternate

Green tube (Sodium heparin), No Gel

3 mL 3 mL
Stability

Room temperature - 12 hour(s)
Refrigerated - 12 hour(s)

Specimen Preparation

  • See comments for additional information
  • Do not freeze
  • Do not centrifuge
  • Mix EDTA tube by gentle inversion immediately after collection
  • Transport to laboratory as soon as possible
  • Keep at room temperature or refrigerate
  • EDTA blood should arrive in the laboratory within 48 hrs from the time of collection

Reasons for Rejection

  • Clotted specimen
  • Delayed or improper handling
  • Frozen specimen
  • Not received within specified time frame

Comments

Interpretation will not be typically provided for these tests unless determined appropriate by Laboratory Director.

Testing will be performed on weekends by notifying lab of intent to order on weekends.  Please notify Lab Services of any weekend testing request before 12pm on Friday by calling 614-722-5477 or 800-934-6575.  Routine samples must be recieved by 3pm on Fridays. 

External samples: only whole blood EDTA, should be received by Laboratory within 48h of blood collection, at either ambient temperature or 4oC, but not frozen.  Sodium heparin anticoagulant will NOT be accepted for external samples.

This test can be available as STAT (TBNKTX) for patients in whom results are urgently needed, and in a non-rush format (TBNK).

Results are typically reported within 24-48h from receipt of sample in the laboratory.

This is a flow cytometric lymphocyte subset quantitation (CD45+ ALC, CD3+, CD4+ and CD8+ T cells, CD19/20+ B cells, CD16/56+ NK cells, CD4:CD8 ratio, CD3+CD4-CD8- DNT cells. This test is useful for the evaluation of patients with primary immunodeficiencies (inborn errors of immunity), patients undergoing solid-organ transplantation (during induction and post-transplant monitoring), patients undergoing hematopoietic cell transplantation (HCT) for assessment of immune reconstitution and competence, patients receiving immunomodulatory therapies, and any clinical context where a more detailed lymphocyte subset evaluation is needed (outside a CBC + differential).

This test can be available as STAT (TBNKTX) for patients in whom results are urgently needed, and in a non-rush format (TBNK).

Results are typically reported within 24-48h from receipt of sample in the laboratory.

CPT Code

  • 86355
  • 86357
  • 86359
  • 86360