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Laboratory Test Directory

Full Thickness Rectal Biopsy-Hirschsprung Evaluation


Name Method Department Units
Full Thickness Rectal Biopsy-Hirschsprung Eval Anatomic Pathology

Specimen Requirements

Tissue (Formalin fixed)

Container Type Container Size Specimen Volume

Formalin (10%) in sterile container

N/A See comments

Room temperature - 24 hour(s)

Specimen Preparation

  • Place specimen in formalin and transport to lab immediately after collection


Specimen size: At least one biopsy measuring 0.3 cm in diameter excised to a depth of 0.05 cm should be collected.

Specimen Transport:

  • Preferred Method Formalin-fixed specimens: Deliver to pathology within 1 hour of collection Call CPA for pick up: CPA can be reached via Vocera ‘CPA Lab’
  • Fresh specimen: Call CPA for a pickup.  Hand deliver to pathologist or designee within 15 minutes of collection

Other requirements: Specimen must be clearly labeled or designated as "Rectal Full Thickness Biopsy" with "Hirschsprung Evaluation" or "Rule out Hirschsprung" noted. Failure to communicate the need for Hirschsprung evaluation by incomplete labeling or incomplete clinical history may result in an indeterminate final diagnosis.If submitting Rectal Suction biopsy for Hirschprung Evaluation, please refer to test Rectal Suction Biopsy-Hirschsprung Evaluation.

*Saturday testing evaluation of specimens received after noon on Friday is available but must be coordinated with on-call pathologist in advance.

No specimen will be rejected. Submitting provider will be notified of potentially inadequate biopsies or labelling errors.

CPT Code

  • 88305
  • 88342
  • added IHC as needed (88341 per additional antibody)