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Laboratory Test Directory

Culture- Bacterial, Blood/Bone Marrow, Routine Bottles

Components

Name Method Department Units
Culture, Blood/Bone Marrow BacT/Alert
CMI, Microbiology

Specimen Requirements

Whole blood

Container Type Container Size Specimen Volume
Preferred

Blood Culture Bottles, Anaerobic and Aerobic

View container
N/A See Comments
Stability

Room temperature - 12 hour(s)

Specimen Preparation

  • Collect using standard sterile technique
  • Remove top of blood culture bottle and clean rubber septume with alcohol pad
  • Transport to laboratory as soon as possible
  • Do not refrigerate
  • Protect from cold.

Bone marrow

Container Type Container Size Specimen Volume
Preferred

Aerobic (green) blood culture bottle

View container
N/A 1-2 mL
Stability

Room temperature - 12 hour(s)

Specimen Preparation

  • Collect using standard sterile technique
  • Remove top of blood culture bottle and clean rubber septume with alcohol pad
  • Transport to laboratory as soon as possible
  • Do not refrigerate
  • Protect from cold.

Reasons for Rejection

  • Not received at proper temperature
  • Not received within specified time frame

Comments

A single blood collection is considered one draw dispensed into one Aerobic (Green)and one Anaerobic (Purple) bottle. The amount of blood collected depends on the weight of the patient following hospital guidelines:

If <1 mL collected, place entire sample in Aerobic (Green) bottle. Bone Marrow: Inoculate entire sample into Aerobic (Green) bottle. Blood culture bottles are incubated 5 days before being finalized. Positive blood culture results are called to physician immediately upon detection. A patient under the age of 4 weeks, in whom a blood culture is being ordered and thus a diagnosis of bacterial sepsis is suspected, should generally receive a full sepsis workup in the ED setting including a lumbar puncture and catheterized urine culture. If this culture is being collected at a lab draw site this recommendation will be faxed and called to the ordering physician's office.

Blood FA

The Blood Culture Identification Panel (Blood FA) is a molecular test that utilizes highly multiplexed PCR to specifically identify 24 organisms and antimicrobial resistance genes within 1-2 hours after the Gram stain reveals organisms in a blood culture bottle. This test allows the preliminary identification of potential pathogens in positive blood cultures. All Blood FA results will be confirmed by culture.

The organisms and the antibiotic resistance markers that are identified by the Blood FA are:

The Blood FA panel will be run on first time positive blood cultures only. It will NOT be run on: body fluids in blood culture media, pathology blood cultures and any bottles that come from outside hospitals.

CPT Code

  • 87040