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Laboratory Test Directory

Chimerism FISH (XX/XY), Post-Transplant Analysis

Components

Name Method Department Units
Chimerism FISH, Post-Transplant Fluorescence in situ hybridization (FISH)
Cytogenetics

Specimen Requirements

Whole blood

Container Type Container Size Specimen Volume
Preferred

Green tube (Sodium heparin), No Gel

3 mL 3 mL
Stability

Room temperature - 24 hour(s)
Refrigerated - 72 hour(s)

Specimen Preparation

  • Do not centrifuge
  • Do not freeze
  • Keep at room temperature

Bone marrow

Container Type Container Size Specimen Volume
Preferred

Green tube (Sodium heparin), No Gel

3 mL 3 mL
Stability

Room temperature - 24 hour(s)
Refrigerated - 72 hour(s)

Specimen Preparation

  • Do not centrifuge
  • Do not freeze
  • Keep at room temperature

Sorted cells

Container Type Container Size Specimen Volume
Preferred

Green tube (Sodium heparin), No Gel

6 mL See Comments
Stability

Room temperature - 24 hour(s)

Specimen Preparation

  • Specimen must be processed within 24 hours of collection
  • Peripheral blood only, bone marrow not acceptable for sorted cells
  • Do not freeze
  • Do not centrifuge
  • Transport to the lab immediately after collection
  • See comment section for collection instructions

Reasons for Rejection

  • Clotted specimen
  • Centrifuged specimen
  • Frozen specimen
  • Wrong collection tube

Comments

Submission of a completed Bone Marrow Transplant (BMT) Genetic Test Requisition Form is required. Please call (614) 722-5321 to obtain the form. Please label all specimens with specimen type (Blood or Bone marrow) along with two patient identifiers (e.g. Name, DOB or MRN).


SORTED CELLS ORDERING: 

If analysis of sorted cells is requested, please indicate on the Test Requisition Form:

  • The types of cells requested: CD3/CD33 vs. CD3/CD33/CD19/CD56.
  • Whether testing is requested on: 1) whole blood AND sorted cells, 2) whole blood ONLY, or 3) sorted cells ONLY.

SORTED CELLS SAMPLE COLLECTION:

  • Only peripheral blood samples can be used for cell sorting. Bone marrow samples CANNOT be used for cell sorting.
  • Blood samples for cell sorting must be collected in NaHep tubes. If whole blood analysis is also requested, then collect a separate EDTA tube.
Sorted Cell Specimen Requirements
Sorted Cell Types Specimen Container & Volume Collection Time
CD3/CD33 Minimum of 4 mL peripheral blood in NaHep (green-top) tube

Monday-Thursday;

Friday collection must be done on Nationwide Children's Main Campus

CD3/CD33/CD19/CD56 Minimum of 8 mL peripheral blood in NaHep (green-top) tube Monday-Friday

 


Bone marrow engraftment analysis by FISH, also known as XX/XY chimerism testing, involves determining the percentage of donor cells present in the recipient after bone marrow transplant (BMT) or hematopoietic stem cell transplantation (HSCT). FISH for XX/XY signals on interphase cells is done to monitor BMT/HSCT engraftment in recipients subsequent to a transplant using a sex-mismatched donor.

For BMT engraftment monitoring through molecular (DNA) genetic markers, please see test code BMPT (Post-Transplant Analysis, Bone Marrow Engraftment).

 

CPT Code

  • 88275
  • 88271