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Laboratory Test Directory

Pre-Transplant Analysis, Bone Marrow Engraftment

Components

Name Method Department Units
Bone Marrow Engraftment - Pre Transplant Polymerase chain reaction (PCR)
Capillary electrophoresis
Microsatellite marker analysis
DNA extraction
Molecular Genetics

Specimen Requirements

Bone marrow, Peripheral blood

Container Type Container Size Specimen Volume
Preferred

Purple tube (EDTA)

4 mL 4 mL-8 mL
Stability

Room temperature - 24 hour(s)
Refrigerated - 72 hour(s)

Specimen Preparation

  • Do not centrifuge
  • Do not freeze
  • Keep at room temperature

Reasons for Rejection

  • Centrifuged specimen
  • Clotted specimen
  • Frozen specimen
  • Wrong collection tube

Comments

Submission of completed Molecular Genetics BMT Requisition Form required, call (614) 722-2866 to obtain. Submission of BOTH the recipient and donor samples are required for this test. Please collect recipient sample PRIOR to pre-transplant treatment. If the donor sample is submitted separately from the recipient sample, a BMT Requisition Form including both donor and recipient information must be submitted with donor sample. All samples should be labeled with specimen type (e.g. blood, bone marrow, stem cells). Please provide expected date of transplant on the BMT Requisition From. Please call Molecular Genetics Lab at (614) 722-2866 with questions. Bone marrow engraftment analysis, also known as chimerism testing, involves determining the percentage of donor cells present in the recipient sample after allogeneic bone marrow transplant (BMT) or hematopoietic stem cell transplant (HSCT). Engraftment monitoring after BMT/HSCT is critical to assess the outcome of transplant and to predict the risk of relapse. Quantitative analysis of polymorphic genetic markers has become the standard technique for engraftment analysis due to its high sensitivity. Pre-transplant bone marrow engraftment analysis determines the polymorphic genetic markers in the recpieint and donor samples, so these genotypes will be available after the transplant for post-transplant engraftment analysis to be done on patient sample. Therefore, donor and recipient specimens must be obtained and genotyped before the transplant event occurs.

CPT Code

  • 81265 (81266 added when there is more than one donor)