|WAS Sanger Sequencing||
Bi-directional sequence analysis
||Send Out Genetics|
Test involves bidirectional sequencing of all 12 exons of the WAS gene. The full coding region of each exon plus ~20 bp of flanking non-coding DNA on either side are sequenced.
|Container Type||Container Size||Specimen Volume|
Purple tube (EDTA)
|6 mL||5 mL|
Room temperature - 48 hour(s)
Refrigerated - 1 week(s)
- Do not centrifuge
- Do not freeze
- Transport to laboratory as soon as possible
Reasons for Rejection
- Centrifuged specimen
- Wrong collection tube
- Frozen specimen
- Clotted specimen
- Delayed or improper handling
Sanger sequencing of the WAS gene associated with Wiskott-Aldrich syndrome, X-linked thrombocytopenia, and X-linked congenital Neutropenia.
Indications for Testing: All male patients with symptoms of Wiskott-Aldrich syndrome or related disorders and with a family history of X-linked inheritance are candidates for this test. Female patients should be considered if they have affected fathers, and/or if they have a strong family history. Males with congenital neutropenia and evidence of X-linked familial inheritance are also candidates.
Complete attached test requisition and submit with specimen.