New Search

Laboratory Test Directory

WAS Sanger Sequencing

Components

Name Method Department Units
WAS Sanger Sequencing Bi-directional sequence analysis
Send Out Genetics

Battery Members

Test involves bidirectional sequencing of all 12 exons of the WAS gene. The full coding region of each exon plus ~10 bp of flanking non-coding DNA on either side are sequenced.

Specimen Requirements

Whole blood

Container Type Container Size Specimen Volume
Preferred

Purple tube (EDTA)

6 mL 5 mL
Alternate

Yellow tube (ACD)

8 mL 5 mL
Stability

Room temperature - 48 hour(s)
Refrigerated - 1 week(s)

Specimen Preparation

  • Do not centrifuge
  • Do not freeze
  • Transport to laboratory as soon as possible

Reasons for Rejection

  • Centrifuged specimen
  • Wrong collection tube
  • Frozen specimen
  • Clotted specimen
  • Delayed or improper handling

Comments

Sanger sequencing of the WAS gene associated with Wiskott-Aldrich syndrome, X-linked thrombocytopenia, and X-linked congenital Neutropenia. 

Indications for Testing: All male patients with symptoms of Wiskott-Aldrich syndrome or related disorders and with a family history of X-linked inheritance are candidates for this test. Female patients should be considered if they have affected fathers, and/or if they have a strong family history. Males with congenital neutropenia and evidence of X-linked familial inheritance are also candidates.

Complete attached test requisition and submit with specimen. 

CPT Code

  • 81406