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Laboratory Test Directory

Tissue Cell Culture, Cytogenetics Lab (Updated)

Components

Name Method Department Units
Tissue Culture Only, Cytogenetics Lab Cell Culture
Cryopreservation
Cytogenetics

Specimen Requirements

Tissue (Fresh)

Container Type Container Size Specimen Volume
Preferred

Tissue culture transport media

N/A 10 mm cube
Alternate

Sterile container with saline

N/A 10 mm cube
Alternate

Ringer's lactate solution in sterile container

N/A 10 mm cube
Stability

Room temperature - 48 hour(s)

Specimen Preparation

  • Do not add fixative
  • Do not formalin fix the sample
  • Keep at room temperature
  • Transport to laboratory as soon as possible
  • Do not freeze

Reasons for Rejection

  • Delayed or improper handling
  • Fixed specimen
  • Formalin Fixed Tissue
  • Frozen specimen

Comments

Submission of a completed test requisition form is required - please use Genetic Test Requisition Form for a postnatally obtained tissue (e.g. skin biopsy, surgically obtained tissue, autopsy tissue from a liveborn patients) and POC Test Requisition Form for tissue obtained from products of conception (POC). Please call Cytogenetics lab at (614) 722-5321 to obtain the requisition form or tissue transport media if needed. For a skin biopsy performed on the main Nationwide Children's Campus (700 Children's Drive), Cytogenetics lab can supply a skin biopsy kit -- please call at least 24 hours in advance to request.

Collected tissue sample should be placed in a sterile container containing tissue transport media or sterile saline, and tissue should be completely immersed in the media/saline. DO NOT freeze or expose to formalin or other fixatives. Transport the specimen to the laboratory as soon as possible at room temperature.

This test is intended for tissue samples that does not have any accompanying cytogenetic test order (e.g. chromosome analysis or microarray analysis) but needs to be cultured for other molecular genetic or biochemical testing (e.g. DNA isolation and storage, familial mutation analysis, or send-out testing) or needs to be cryopreserved for possible future testing. This test includes cryopreservation of cultured cells.

If additional in-house or send-out testing on cultured fibroblasts is desired, please clearly indicate the desired testing on the requisition form and call Cytogenetics lab at (614) 722-5321 and speak to a laboratory genetic counselor to coordinate. Otherwise, cultured cells will be cryopreserved for future testing. If tissue from products of conception (POC) is submitted and molecular genetic testing is requested on cultured cells, then submission of a maternal blood sample (4 mL in EDTA tube) is highly recommended to perform maternal cell contamination study.

CPT Code

  • 88233
  • 88240