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Laboratory Test Directory

Thrombin Time (Mix with Protamine Sulfate)


Name Method Department Units
Thrombin Time Protamine Clot detection
Core Lab, Coagulation sec

Specimen Requirements

Platelet poor plasma

Container Type Container Size Specimen Volume

Blue plastic tube (Citrate)

2.7 mL 2.7 mL

Frozen - 2 week(s)


Blue plastic tube (Citrate)

1.8 mL 1.8 mL

Frozen - 2 week(s)

Specimen Preparation

  • Fill tubes to appropriate mark.
  • Mix by gentle inversion immediately after collection
  • Centrifuge, remove plasma/serum and freeze
  • Plasma must be platelet free

Reasons for Rejection

  • Over or under filled tube
  • Clotted specimen
  • Not received within specified time frame
  • Gross hemolysis
  • Wrong collection tube
  • Capillary specimen
  • Specimen collected in glass tube


Baseline Thrombin Time (LIS code: TT; CPT code: 85670) MUST be performed prior to this test. This test should only be performed if the baseline thrombin time is higher than the normal range limit and presence of heparin is suspected. Requests for performance of this test from Friday after 3:00 p.m. until 7 a.m. Monday morning must be approved by a pathologist. Indicate collection method on sample and if patient is receiving heparin therapy. Centrifuge citrate tubes according to Lab policy, remove platelet-poor plasma and freeze.

CPT Code

  • 85670