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Laboratory Test Directory

Single Kinase Fusion Detection, Singleplex RT-PCR

Components

Name Method Department Units
Single Kinase Fusion Detection, Singleplex RT-PCR Reverse transcriptase polymerase chain reaction (RT-PCR)
Sanger sequencing
Molecular Genetics

Specimen Requirements

Bone marrow, Whole blood

Container Type Container Size Specimen Volume
Preferred

Purple tube (EDTA)

4 mL 4 mL
Stability

Room temperature - 24 hour(s)

Specimen Preparation

  • Transfer 4 mL bone marrow or whole blood to COG ALL shipping media
  • If COG ALL shipping media is unavailable, transfer 4 mL bone marrow or whole blood to tissue culture media

Reasons for Rejection

  • Delayed or improper handling
  • Wrong collection tube

Comments

Recent studies have identified a subtype of newly diagnosed and relapsed B-progenitor acute lymphoblastic leukemia (B-ALL) children with a gene expression profile suggestive of an alternate kinase fusion in the absence of a classical, t(9;22) BCR-ABL1 rearrangement. Please click on the Lab Form link in the Forms Section to print and complete the Pediatric Oncology Test Requisition Form. Submission of a completed Test Requisition Form is required, and submission of informed consent form is recommended (but not required). The specific fusion transcripts tested in this assay are listed on page 2 of this Test Requisition Form. Fusion partners are confirmed by DNA sequence analysis of the RT-PCR product. All three multiplex assays may be ordered at once [test code ALLFUSMA, includes ABL1 fusion multiplex, JAK fusion multiplex and Kinase fusion multiplex]. Each multiplex assay may also be ordered individually if previous testing indicates a targeted approach can be taken. This specific Single Kinase Fusion Detection, Singleplex RT-PCR test (test code KINFS) only assesses a specific single fusion specified by the provider on the Test Requisition form.  Pathology review by a Laboratory Services pathologist will be performed on all samples to verify tumor percentage. A minimum of 10% blasts is required to report a result within a mixed cell population. A blast percentage of less than 10% may be reported as unsatisfactory (no results reported). Please send 4mL bone marrow OR 4mL involved peripheral blood in Children's Oncology Group ALL shipping media. This media is strongly preferred in order to preserve the available RNA in the sample. If COG ALL shipping media is unavailable please send in tissue culture media. Ship sample at ambient temperature within 24 hours of collection. Testing is time sensitive due to RNA instability. If shipping is delayed, please call the laboratory. Please call (614) 722-2866 prior to shipping and provide tracking number. Ship sample for overnight delivery. Saturday deliveries are accepted. Please check “Saturday Delivery” on shipment label. A diagnostic or relapse sample previously submitted under a COG protocol can also be used in most cases (some cases may require approval from the study chair); please call the laboratory to confirm sample availability. RNA can only be accepted if previously isolated in a CAP/CLIA laboratory AND requires submission of a cytospin from the exact blood or bone marrow pull (used for internal hematopathologic review of blast assessment). Please call the laboratory to discuss prior to sending RNA sample. Isolated RNA should be sent frozen on dry ice.

Forms

Lab Form

CPT Code

  • 81479