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Laboratory Test Directory

RT-PCR Fusion Confirmation

Components

Name Method Department Units
RT-PCR Fusion Confirmation Reverse transcriptase polymerase chain reaction (RT-PCR)
Sanger sequencing
Molecular Genetics

Specimen Requirements

Bone marrow, Whole blood

Container Type Container Size Specimen Volume
Preferred

Purple tube (EDTA)

4 mL 4 mL
Stability

Room temperature - 24 hour(s)

Specimen Preparation

  • Transfer 4 mL bone marrow or whole blood to COG ALL shipping media
  • If COG ALL shipping media is unavailable, transfer 4 mL bone marrow or whole blood to tissue culture media
  • Do not freeze
  • Tumor sample must contain a minimum of 10% tumor

Tissue (Fresh)

Container Type Container Size Specimen Volume
Preferred

Tissue culture transport media

N/A 5mm x 5mm or larger
Stability

Room temperature - 24 hour(s)

Specimen Preparation

  • Do not add fixative
  • Transport to laboratory as soon as possible
  • Do not freeze
  • Tumor sample must contain a minimum of 10% tumor

Tissue (Snap-frozen)

Container Type Container Size Specimen Volume
Preferred

Tissue cassette

N/A 5mm x 5mm or larger
Alternate

Cryogenic tube

N/A 5mm x 5mm or larger
Stability

Frozen - 12 month(s)

Specimen Preparation

  • Keep frozen
  • Protect from heat
  • Immediately place on dry ice and transport frozen
  • Transport to laboratory as soon as possible
  • Tumor sample must contain a minimum of 10% tumor

OCT-embedded tissue

Container Type Container Size Specimen Volume
Preferred

Tissue cassette

N/A 5mm x 5mm or larger
Alternate

Cryogenic tube

N/A 5mm x 5mm or larger
Stability

Frozen - 12 month(s)

Specimen Preparation

  • Keep frozen
  • Protect from heat
  • Immediately place on dry ice and transport frozen
  • Transport to laboratory as soon as possible
  • Tumor sample must contain a minimum of 10% tumor

Reasons for Rejection

  • Inadequate tissue
  • Delayed or improper handling
  • Tissue degradation

Comments

Please click on the Lab Form Link in the Forms Section to print and complete the Pediatric Oncology Test Requisition Form. Submission of completed Genetic Test Requisition Form is required, and submission of informed consent form is recommended.

This test is a targeted RT-PCR analysis used to confirm the presence of a specific gene fusion (translocation) in a tumor sample that was previously identified by a research testing or by another clinical laboratory. When a gene fusion is detected by RT-PCR, a Sanger sequencing of PCR product will be performed to confirm the presence of gene fusion in the submitted sample.

Please submit a copy of the previous tumor fusion test result, if available. Submitted tumor sample must contain at least 10% tumor based on Nationwide Children's Laboratory internal pathology review. Please send frozen samples (e.g. snap-frozen tissue or OCT-embedded tissue) buried in adequate amount of dry ice and ship by overnight courier. Please note that paraffin-embedded (FFPE) tissue is NOT ACCEPTED for this testing at this time.

For questions, please call (614) 722-2866.

Forms

Lab Form

CPT Code

  • 81479