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Laboratory Test Directory

Paroxysmal Nocturnal Hemoglobinuria, High Sensitivity, WBC


Name Method Department Units
PNH, WBC Quantitative Flow Cytometry
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Specimen Requirements

Whole blood

Container Type Container Size Specimen Volume

Purple tube (EDTA)

4 mL 4 mL

Green tube (Sodium heparin), No Gel

6 mL 6 mL

Green tube (Lithium heparin), No Gel

6 mL 6 mL

Room temperature - 1 day(s)
Refrigerated - 3 day(s)

Specimen Preparation

  • Mix by gentle inversion immediately after collection
  • Transport to laboratory as soon as possible

Reasons for Rejection

  • Frozen specimen
  • Clotted specimen
  • Hemolyzed specimen
  • Bone Marrow


Specimen must be analyzed within 72 hours of collection. Must be sent out same day as collected. Bone marrow is unacceptable. This assay includes diagnostic WBC analysis using FLAER & CD157 as GPI-linked markers with CD15 and CD64 as lineage specific markers.

CPT Code

  • 86356 (x5)