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Laboratory Test Directory

Chromosome Analysis, Percutaneous Umbilical Blood (PUBS)

Components

Name Method Department Units
Percutaneous Umbilical Blood Chromosome Analysis Chromosomal analysis
Cytogenetics

Specimen Requirements

Whole blood

Container Type Container Size Specimen Volume
Preferred

Green tube (Sodium heparin), No Gel

3 mL 1 mL- 3 mL
Stability

Room temperature - 24 hour(s)

Specimen Preparation

  • Do not freeze
  • Do not centrifuge
  • Do not refrigerate
  • Keep at room temperature

Reasons for Rejection

  • Centrifuged specimen
  • Collected in tube with gel separator
  • Wrong collection tube
  • Delayed or improper handling
  • Frozen specimen
  • Clotted specimen

Comments

Submission of a completed Prenatal Genetic Test Requisition Form is required. To obtain the requisition form, please call the Cytogenetics Laboratory at (614) 722-5321. Please collect fetal blood sample in Sodium Heparin tube (dark green-top tube without gel separator). Please do not use Lithium Heparin tube or tubes with other fixatives. Clearly indicate "PUBS sample" on the requisition form. 

This test performs STAT chromosome analysis on the fetal blood sample obtained by percutaneous umbilical blood sampling (PUBS) procedure. This test evaluates chromosomes in 20 cells (cultured lymphocytes) from fetal blood sample. This test is used to diagnose or rule out numerical and structural chromosomal abnormalities such as aneuploidy, chromosome translocation, and chromosome inversion.

Verbal STAT preliminary result (non-high resolution chromosome result) will be available in 2 business days (within 72 hours) from time specimen received by the laboratory. Final written chromosome result (high resolution chromosome result) is typically available in 7 days.

If evaluation of extra cells (beyond the routine 20 cells) is desired to rule out low-level mosaicism, please request "Mosaicism Study" on the lab order and 50 cells will be evaluated at extra charge.

If patient has a family history of known chromosome abnormality, please provide the family member's chromosome result information (attach a copy of test result if available). If the proband was tested at Nationwide Children's Hospital Cytogenetics Laboratory, please provide the proband name, date of birth, and accession number (if available).

CPT Code

  • 88230
  • 88262
  • 88289