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Laboratory Test Directory

Oxidative Burst Assay

Components

Name Method Department Units
Oxidative Burst Assay Flow cytometry
Core Lab, Flow Cytometry NOI (Neutrophil Oxidative Index)

Specimen Requirements

Whole blood

Container Type Container Size Specimen Volume
Preferred

Purple tube (EDTA)

3 mL 3 mL
Stability

Room temperature - 24 hour(s)

Alternate

Purple tube (EDTA)

Micro sample tube 0.5 mL
Stability

Room temperature - 24 hour(s)

Alternate

Green tube (Sodium heparin), No Gel

3 mL 3 mL
Specimen Preparation

  • Do not centrifuge
  • Keep at room temperature
  • Mix by gentle inversion immediately after collection
  • Transport to the lab immediately after collection
  • Do not refrigerate

Reasons for Rejection

  • Clotted specimen
  • Wrong collection tube
  • Not received within specified time frame
  • Not received at proper temperature
  • Specimen received on wrong day of week.
  • Refrigerated specimen

Comments

Testing is setup Monday-Friday on dayshift but not on national holidays-plan collection accordingly.  Samples collected on Friday must be received in the lab before 1300.  Contact Flow Cytometry Lab at 614-722-5482 with questions about the setup schedule and specimen acceptibility. Testing must be completed within 24 hours after specimen collection. Test is not setup on Saturday, Sunday or National holidays.

Patient must have an absolute neutrophil count (ANC) greater than 1.5 before oxidative burst assay will be performed. Samples with an ANC less than 1.5 will be credited.

Abnormal results may generate a Pathologist consultation (billed through Pediatric Pathology Associates (PPAC)). If repeat testing on a new sample is requested by the pathologist for confirmation of abnormal results, another test charge for the oxidative burst assay will be generated and billed. For patients that display results consistent with Chronic Granulomatous Disease (CGD), Myeloperoxidase (MPO) by flow cytometry will be performed and charged to the patient (CPT code: 88185).

CPT Code

  • 88184
  • 88185