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Laboratory Test Directory

Neuroblastoma Microarray Analysis (Updated)

Components

Name Method Department Units
Neuroblastoma Microarray Analysis Microarray analysis
Molecular Genetics

Specimen Requirements

Bone marrow

Container Type Container Size Specimen Volume
Preferred

Purple tube (EDTA)

4 mL 4 mL
Stability

Room temperature - 24 hour(s)
Refrigerated - 72 hour(s)

Specimen Preparation

  • Do not freeze
  • Do not centrifuge
  • Keep at room temperature or refrigerate
  • Tumor sample must contain a minimum of 40% tumor

Tissue (Fresh)

Container Type Container Size Specimen Volume
Preferred

Sterile container with saline

N/A
Stability

Room temperature - 24 hour(s)
Refrigerated - 72 hour(s)

Specimen Preparation

  • Transport to the lab immediately after collection
  • Keep at room temperature or refrigerate

Reasons for Rejection

  • Wrong type of specimen
  • Inadequate tissue
  • Delayed or improper handling
  • Post-treatment tumor specimen
  • Tissue degradation

Comments

Please click on the Lab Form Link in the Forms Section to print and complete the Pediatric Oncology Test Requisition Form. Submission of completed Test Requisition Form is required, and submission of informed consent form is recommended (but not required).

Please send all samples via overnight delivery. Saturday deliveries are accepted; please check "Saturday Delivery" on shipment label. Please call (614) 722-2866 with questions.


The neuroblastoma microarray analysis will detect and report segmental abnormality in at least one of the following regions of interest: 1p loss, 1q gain, 2p gain, 3p loss, 4p loss, 11q loss, or 17q gain and loss of heterozygosity of greater than 5 Mb. Depending on the aboration, a normal (germline sample) ?may be required to determine if the aboration is somatic or germline in origin.

A segmental abnormality is defined as a region that is 1 Mb or larger. Whole chromosome and whole arm aberrations are not considered segmental findings; however, it is possible to have a segmental finding within a whole chromosome or whole arm aberration if a region >1 Mb has an additional gain or loss beyond the overall copy number for that chromosome arm (e.g. If 17q shows an overall gain of one copy but a >1 Mb region within the arm shows an additional copy number gain, this would be considered a segmental gain).

PLEASE NOTE:

1) At least 40% tumor must be present in the submitted tumor sample. A tumor percentage of <40% may be reported as unsatisfactory (no results reported). An internal pathology review will be performed on submitted tumor samples to verify tumor percentage.

2) Tumor sample collected during or after treatment (e.g. post-chemotherapy) CANNOT be accepted. Pre-treatment relapse tumor samples can be submitted.

3) If concerning aboration is found in the tumor sample, a germline sample from the individual (buccal swab or uninvolved peripheral blood sample (4 mL EDTA)) may be requested to?dermine if the aboration is somatic or germline in origin.

Forms

Lab Form

CPT Code

  • 81406