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Laboratory Test Directory

Neuroblastoma Loss of Heterozygosity (LOH)

Components

Name Method Department Units
Neuroblastoma Loss of Heterozygosity (LOH) Capillary electrophoresis
Polymerase chain reaction (PCR)
Microsatellite marker analysis
Molecular Genetics

Specimen Requirements

Whole blood

Container Type Container Size Specimen Volume
Required

Purple tube (EDTA)

4 mL 4 mL
Stability

Room temperature - 24 hour(s)
Refrigerated - 72 hour(s)

Specimen Preparation

  • Do not centrifuge
  • Do not freeze
  • Keep at room temperature or refrigerate

Paraffin embedded tissue

Container Type Container Size Specimen Volume
Alternate

Paraffin block

N/A
Stability

Room temperature - 6 month(s)

Specimen Preparation

  • Keep at room temperature
  • Protect from heat

Snap frozen specimen

Container Type Container Size Specimen Volume
Preferred

Sterile container

N/A
Stability

Frozen - 24 hour(s)

Specimen Preparation

  • Freeze immediately after collection.
  • Immediately place on dry ice and transport frozen

Reasons for Rejection

  • Inadequate tissue
  • Tissue degradation
  • Delayed or improper handling
  • Wrong type of specimen
  • Post-treatment tumor specimen

Comments

Please click on the Lab Form Link in the Forms Section to print and complete the Pediatric Oncology Test Requisition Form. Submission of completed Test Requisition Form is required, and submission of informed consent form is recommended (but not required).

Children with neuroblastoma (NBL) are assigned a specific risk group (low, intermediate, or high) based on a variety of clinical and biological factors. These can include age at time of diagnosis, INSS staging, MYCN amplification status, DNA ploidy, and Shimada histology. Most children with NBL will be enrolled in Children's Oncology Group (COG) protocols. Poor outcome for patients with NBL can be associated with loss of heterozygosity (LOH) at chromosome bands 1p36 and 11q23. LOH testing is currently NOT available through a COG trial and can be ordered clinically at Nationwide Children's Hospital Clinical Genetics Laboratory. Snap-frozen, paraffin-embedded, OCT-embedded tumors or involved bone marrow can be submitted for analysis. Submission of uninvolved (germline) sample is also required. Samples submitted under a COG protocol can also be used in most cases (some cases may require approval from the study chair); please call the Clinical Genetics Laboratory at (614) 722-2866 to confirm sample availability.

PLEASE NOTE:

1) at least 40% tumor must be present in the submitted tumor sample. A tumor percentage of <40% may be reported as unsatisfactory (no results reported). An internal pathology review will be performed on submitted tumor samples to verify tumor percentage.

2) Tumor sample collected during or after treatment (e.g. post-chemotherapy) CANNOT be accepted. Pre-treatment relapse tumor samples can be submitted. Please send all samples via overnight delivery. Saturday deliveries are accepted; please check Saturday Delivery on shipment label.

Please call (614) 722-2866 with questions.

Forms

Lab Form

CPT Code

  • 81479