Tritiated thymidine incorporation
PHA stimulation, Con A stimulation, PWM stimulation
|Container Type||Container Size||Specimen Volume|
Green tube (Sodium heparin), No Gel
|10 mL||10 mL|
Room temperature - 24 hour(s)
- Keep at room temperature
- Do not centrifuge
- Do not freeze
- Transport to laboratory as soon as possible
Reasons for Rejection
- Not collected in Sodium Heparin tube
- Frozen specimen
- Refrigerated specimen
- Clotted specimen
- Hemolyzed specimen
- Centrifuged specimen
Can only be drawn Monday thru Thursday. All samples must be collected between 10:30 A.M. and 5 P.M. They must be received by Sendout lab by 5:10 P.M.(same day of collection) to ensure delivery to reference lab within 24 hours. To ensure samples are not rejected as QNS due to lack of Lymphocytes please refer to the "chart" below and submit sample based on absolute lymphocyte count.
Draw the following recommended volume for EACH of the following tests NK function, Mitogen Stimulation, and Antigen stimulation.
Absolute Lymphocyte Count(cells/ul) ml of blood needed
For counts below 200/ul, consider waiting until count increases. For any further questions please contact Cincinnati Children's Immunology lab at 513-636-4685.
- 86353 (x3)