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Laboratory Test Directory

Mitogen Stimulation (Updated)

Components

Name Method Department Units
Mitogen Stimulation Tritiated thymidine incorporation
Send Out

Battery Members

PHA stimulation, Con A stimulation, PWM stimulation

Specimen Requirements

Whole blood

Container Type Container Size Specimen Volume
Preferred

Green tube (Sodium heparin), No Gel

10 mL 10 mL
Stability

Room temperature - 24 hour(s)

Specimen Preparation

  • Keep at room temperature
  • Do not centrifuge
  • Do not freeze
  • Transport to laboratory as soon as possible

Reasons for Rejection

  • Not collected in Sodium Heparin tube
  • Frozen specimen
  • Refrigerated specimen
  • Clotted specimen
  • Hemolyzed specimen
  • Centrifuged specimen

Comments

Can only be drawn Monday thru Thursday. All samples must be collected between 10:30 A.M. and 5 P.M. They must be received by Sendout lab by 5:10 P.M.(same day of collection) to ensure delivery to reference lab within 24 hours. To ensure samples are not rejected as QNS due to lack of Lymphocytes please refer to the "chart" below and submit sample based on absolute lymphocyte count. Draw the following recommended volume for EACH of the following tests NK function, Mitogen Stimulation, and Antigen stimulation.


Absolute Lymphocyte Count(cells/ul)     ml of blood needed
        >1000                                                             5.0
          1000                                                             5.0
            900                                                             5.5
            800                                                             6.0
            700                                                             6.5
            600                                                             7.5
            500                                                             9.0
            400                                                            11.0
            300                                                            15.0
            200                                                            25.0
            100                                                            45.0
          <100                                                            50.0


For counts below 200/ul, consider waiting until count increases. For any further questions please contact Cincinnati Children's Immunology lab at 513-636-4685.

CPT Code

  • 86353 (x3)