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Laboratory Test Directory

Bone Marrow Immunophenotype

Components

Name Method Department Units
Leukocyte Immunophenotype Flow cytometry
Core Lab, Flow Cytometry %

Battery Members

CD1a,2,3,4,5,7,8,10,11b,11c,13,15,16,19,20,23,33,34,38,41,45,56,57,64,71,117,123,Kappa, Lambda HLA:DR,

Specimen Requirements

Bone marrow, Whole blood

Container Type Container Size Specimen Volume
Preferred

Green tube (Sodium heparin), No Gel

10 mL 3 mL
Alternate

Purple tube (EDTA)

3 mL 3 mL
Stability

Refrigerated - 48 hour(s)

Specimen Preparation

  • Do not centrifuge
  • Mix by gentle inversion immediately after collection
  • Transport to laboratory as soon as possible
  • Keep refrigerated

Bone marrow smear

Container Type Container Size Specimen Volume
Preferred

Air-dried smears

4 4
Stability

Room temperature - 14 day(s)

Specimen Preparation

  • Transport to laboratory as soon as possible

Reasons for Rejection

  • Not received within specified time frame
  • Clotted specimen
  • Wrong collection tube

Comments

The preferred specimen type is bone marrow. Immunophenotyping may be performed on peripheral blood (EDTA) if there is a large number of Blast cells (population of interest) present. Bone Marrow smears submitted to the lab will be used for assessing cell morphology only; immunophenotyping cannot be performed on bone marrow smears. Call 614-722-5482 for more information about sample sources and additional markers. Result will include pathologist interpretation. Reflexive testing may include Cytochemical stains (CPT 88319 x number of stains ordered), Iron stain (CPT 85536), and/or additional Surface or Intracellular markers (CPT 88185) ordered by the Pathologist for confirmation of diagnosis. A Pathologist's interpretation (billed from Pediatric Pathology Associates (PPAC)) will be ordered and charged to the patient.

CPT Code

  • 88184
  • 88185 (x30)