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Laboratory Test Directory

HIV-1 RNA Quantitative by RT-PCR

Components

Name Method Department Units
HIV-1 RNA by PCR, Quantitative CMI, Molecular Infectious Disease

Battery Members

HIV copies/mL, HIV log10 copies/mL

Specimen Requirements

Whole blood

Container Type Container Size Specimen Volume
Preferred

Purple tube (EDTA)

6 mL 6 mL
Stability

Room temperature - 6 hour(s)
Refrigerated - 24 hour(s)

Specimen Preparation

  • Transport to the lab immediately after collection
  • If delay in transport greater than 1 hour, refrigerate

Plasma Spun

Container Type Container Size Specimen Volume
Preferred

Purple tube (EDTA)

6 mL 3 mL
Stability

Room temperature - 24 hour(s)
Refrigerated - 5 day(s)
Frozen - Indefinitely month(s)

Specimen Preparation

  • Centrifuge, remove plasma/serum and refrigerate
  • Transport to laboratory as soon as possible

Reasons for Rejection

  • Wrong collection tube
  • Not received at proper temperature
  • Not received within specified time frame
  • Not centrifuged within time frame
  • Clotted specimen
  • Frozen whole blood
  • Thawed and refrozen specimen

Comments

Clearly mark time of collection on container. This test detects HIV-1 RNA copies in the range of 40-10,000,000/mL of plasma (1.6-7.00 log10 copies/mL).

CPT Code

  • 87536