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Laboratory Test Directory

HIV-1 & HIV-2 Antigen/Antibody Screen

Components

Name Method Department Units
HIV 1 and HIV 2 Ag/Ab Screen Chemiluminescent Microparticle Immunoassay (CMIA)
CMI, Immunology

Specimen Requirements

Whole blood

Container Type Container Size Specimen Volume
Preferred

Purple tube (EDTA)

3 mL 3 mL
Alternate

Red Tube (no anticoagulant) with Gel

3 mL 3 mL
Alternate

Gold tube, (SST) with Gel

3.5 mL 3 mL
Alternate

Green tube (Lithium heparin), No Gel

3 mL 3 mL
Alternate

Green tube (Lithium heparin), with Gel

3 mL 3 mL
Alternate

Green tube (Sodium heparin), No Gel

3 mL 3 mL
Alternate

Red tube, (no anticoagulant), No Gel

3 mL 3 mL
Stability

Room temperature - 24 hour(s)
Refrigerated - 3 day(s)

Specimen Preparation

  • Collect by venipuncture
  • Mix EDTA or heparin tube by gentle inversion immediately after collection.
  • Allow to clot completely at room temperature
  • Transport to laboratory as soon as possible
  • If delay in transport greater than 1 hour, refrigerate

Serum Spun, Plasma Spun

Container Type Container Size Specimen Volume
Preferred

Purple tube (EDTA)

3 mL 3 mL
Alternate

Red Tube (no anticoagulant) with Gel

3 mL 3 mL
Alternate

Gold tube, (SST) with Gel

3.5 mL 3 mL
Alternate

Green tube (Lithium heparin), No Gel

3 mL 3 mL
Alternate

Green tube (Lithium heparin), with Gel

3 mL 3 mL
Alternate

Green tube (Sodium heparin), No Gel

3 mL 3 mL
Alternate

Red tube, (no anticoagulant), No Gel

3 mL 3 mL
Stability

Refrigerated - 7 day(s)
Frozen - Indefinite month(s)
Room temperature - 3 day(s)

Specimen Preparation

  • Transport to laboratory as soon as possible
  • Centrifuge, remove plasma/serum and refrigerate

Reasons for Rejection

  • Wrong collection tube
  • Not received at proper temperature
  • Not received within specified time frame

Comments

All positives screens will be sent to a reference lab for confirmation testing by an antibody differentiation assay, at additional charge. The confirmatory testing REQUIRES EDTA plasma.

CPT Code

  • 87389
  • Varies