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Laboratory Test Directory

Hepatitis B Surface Antigen

Components

Name Method Department Units
Hepatitis B Surface, Ag Chemiluminescent Microparticle Immunoassay (CMIA)
CMI, Immunology Negative or Positive

Battery Members

If the antigen screen is reactive, specimens will be sent out for confirmatory testing.

Specimen Requirements

Whole blood

Container Type Container Size Specimen Volume
Preferred

Red tube, (no anticoagulant), No Gel

3 mL 3 mL
Alternate

Gold tube, (SST) with Gel

3.5 mL 3 mL
Alternate

Green tube (Lithium heparin), No Gel

3 mL 3 mL
Alternate

Green tube (Lithium heparin), with Gel

3 mL 3 mL
Alternate

Green tube (Sodium heparin), No Gel

3 mL 3 mL
Alternate

Purple tube (EDTA)

3 mL 3 mL
Stability

Room temperature - 24 hour(s)
Refrigerated - 6 day(s)

Specimen Preparation

  • Collect by venipuncture
  • Mix EDTA or heparin tube by gentle inversion immediately after collection.
  • Allow to clot completely at room temperature
  • Transport to laboratory as soon as possible

Serum Spun, Plasma Spun

Container Type Container Size Specimen Volume
Preferred

Red tube, (no anticoagulant), No Gel

3 mL 3 mL
Alternate

Gold tube, (SST) with Gel

3.5 mL 3 mL
Alternate

Green tube (Lithium heparin), No Gel

3 mL 3 mL
Alternate

Green tube (Lithium heparin), with Gel

3 mL 3 mL
Alternate

Green tube (Sodium heparin), No Gel

3 mL 3 mL
Alternate

Purple tube (EDTA)

3 mL 3 mL
Stability

Room temperature - 24 hour(s)
Refrigerated - 6 day(s)
Frozen - Indefinite month(s)

Specimen Preparation

  • Transport to laboratory as soon as possible
  • Centrifuge, remove plasma/serum and refrigerate

Reasons for Rejection

  • Gross hemolysis
  • Gross icterus
  • Gross lipemia
  • Bacterial contamination

Comments

Reactive HBsAG serum specimens will be sent out to ARUP reference lab for confirmatory testing. (Test number 0020128)

CPT Code

  • 87340