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Laboratory Test Directory

Hemoglobin Electrophoresis


Name Method Department Units
Hemoglobin Electrophoresis Capillary electrophoresis
Core Lab, Special Hematology %

Battery Members

HbA, HbF, HbS, HbC, HbA2, Hb Other

Specimen Requirements

Whole blood

Container Type Container Size Specimen Volume

Purple tube (EDTA)

3 mL 3 mL

Purple tube (EDTA)

Micro sample tube 0.5 mL

Refrigerated - 7 day(s)
Room temperature - 8 hour(s)

Specimen Preparation

  • Do not centrifuge
  • Transport to laboratory as soon as possible
  • Mix by gentle inversion immediately after collection

Reasons for Rejection

  • Clotted specimen
  • Over or under filled tube
  • Not received within specified time frame
  • Not received at proper temperature
  • Wrong collection tube


Pathologist approval is required for weekend, evening/late night and holiday STAT requests. This assay is used for all patients, regardless of age. Results will be affected if the patient has been transfused with red blood cells within the past 3-4 months. Provide patient's clinical history including newborn screen report from Ohio Department of Health, if available. Reflexive testing may include a CBC (CPT 85027), Sickle Solubility Screen (CPT 85660), and/or Acid Agar Electrophoresis (CPT 83020)ordered by the Pathologist for confirmation of diagnosis. A Pathologist's consult (billed from Pediatric Pathology Associates (PPAC)) will be ordered and charged to the patient if results are abnormal. A Pathologist's consult/interpretation may extend the expected turnaround time.

CPT Code

  • 83020