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Laboratory Test Directory

Culture- Fungal, Dermatophyte

Components

Name Method Department Units
Fungal Culture, Dermatophyte Standard fungal culture media and techniques
CMI, Microbiology

Specimen Requirements

Skin or nail scrapings or hair shaft, Scalp

Container Type Container Size Specimen Volume
Preferred

Sterile container

N/A
Alternate

Dual culture swab system

N/A
Stability

Room temperature - 24 hour(s)
Refrigerated - 24 hour(s)

Specimen Preparation

  • Transport to laboratory as soon as possible

Reasons for Rejection

  • Not received within specified time frame
  • Wrong type of specimen
  • Specimen received on or between glass slides

Comments

Direct smear (KOH Prep) not included and must be ordered separately (KOHP); KOH prep cannot be performed on swab specimens. Appropriate specimens for dermatophyte culture include skin, nail, or scalp/hair.Collect skin scrapings at the advancing margin of lesion by using the blunt edge of a scalpel blade or edge of a glass slide and collecting the scrapings on a piece of clean paper (preferably colored paper). Fold the paper to contain the scrapings and secure with a paper clip or in a clean envelope, and then place the paper into a urine container. DO NOT send the scalpel blade or glass slide with the scrapings. Collect scalp specimens by plucking hair shafts (cut hair not acceptable) and also include scrapings at the base of the lesion; submit in folded paper as above for skin. A swab specimen may also be submitted in addition if scalp lesion is moist. Alternative collection method for skin and hair/scalp utilizes the sterile, disposable toothbrush scrub technique. Collect nails specimens as follows: Clean nail with 70% alcohol. For a specimen of the dorsal plate, scrape the outer surface and discard the scrapings. Then scrape the deeper portion for a specimen. Remove a portion of debris from under the nail with a scalpel. Collect the whole nail or nail clippings. Place all material in a folded piece of paper (colored is preferable) secured with a paper clip or in a clean envelope and place directly in a urine container labeled with the patient's data. Swab collection generally not recommended unless collecting moist lesion; never moisten a swab with tap water—use sterile water or saline instead.

CPT Code

  • 87101