Enzyme-linked immunosorbent assay (ELISA)
ENA, RNP, Sm
|Container Type||Container Size||Specimen Volume|
Red tube, (no anticoagulant), No Gel
|3 mL||2 mL|
Room temperature - 8 hour(s)
Refrigerated - 48 hour(s)
Frozen - indefinite day(s)
- Allow specimen to clot for one hour at room temperature
- Centrifuge, remove serum and refrigerate
Reasons for Rejection
- Plasma unacceptable
- Frozen whole blood
- Wrong collection tube
- Capillary specimen
- Bacterial contamination
- Heat treated specimen
- Gross lipemia
- Gross hemolysis
Includes Smith and Ribonuclear Protein Antibodies. Positive results are answered with units
A positive result in the RNP ELISA indicates the presence of antibodies reactive with the RNP/Sm complex but cannot distinguish between anti-Sm and anti-RNP activity.
The Sm ELISA is intended for the measurement of Sm antibody activity and is considered a marker for SLE.
If the patient is positive for Sm antibodies, it is very likely that RNP antibodies are also present (it is rare to see anti-Sm in the absence of RNP antibodies).
If a sample is negative in the Sm ELISA but positive in the RNP ELISA, then the reactivity is largely or exclusively due to RNP antibodies.
For results interpretation, consultation with Rheumatology is suggested.
- 86235 (x2)