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Laboratory Test Directory

DNA Isolation and Storage

Components

Name Method Department Units
DNA Isolation and Storage DNA extraction
Cryopreservation
Molecular Genetics

Specimen Requirements

Whole blood

Container Type Container Size Specimen Volume
Preferred

Purple tube (EDTA)

4 mL 4 mL-8 mL
Stability

Room temperature - 24 hour(s)
Refrigerated - 72 hour(s)

Specimen Preparation

  • Do not centrifuge
  • Do not freeze

Amniotic fluid

Container Type Container Size Specimen Volume
Preferred

Sterile container

N/A 20 mL-30 mL
Stability

Room temperature - 24 hour(s)

Specimen Preparation

  • Keep at room temperature
  • Do not centrifuge
  • Do not freeze
  • Transport to laboratory as soon as possible

Tissue (Fresh)

Container Type Container Size Specimen Volume
Preferred

Tissue culture transport media

N/A 20-30mm cube
Preferred

Sterile container with saline

N/A 20-30mm cube
Stability

Room temperature - 24 hour(s)
Refrigerated - 24 hour(s)

Specimen Preparation

  • Do not add fixative
  • Do not freeze
  • Do not formalin fix the sample
  • Keep at room temperature or refrigerate
  • Transport to the lab immediately after collection

Tissue (Frozen)

Container Type Container Size Specimen Volume
Preferred

Sterile container

N/A 20-30 mm cube
Stability

Frozen - 12 month(s)

Specimen Preparation

  • Keep frozen
  • Do not formalin fix the sample
  • Immediately place on dry ice and transport frozen

Cultured amniocytes

Container Type Container Size Specimen Volume
Preferred

T25 post confluent flasks

N/A 2 flasks
Specimen Preparation

  • Keep at room temperature
  • Do not freeze
  • Transport to laboratory as soon as possible

Reasons for Rejection

  • Serum sample
  • Clotted specimen
  • Wrong collection tube
  • Tissue degradation
  • Delayed or improper handling
  • Fixed specimen

Comments

Genomic DNA can be extracted from submitted sample and stored for future testing (to be done at Nationwide Children's Molecular Genetics Lab or sent out to outside reference lab). Acceptable sample types include whole blood in EDTA tube, saliva in Oragene collection tube, amniotic fluid, cultured amniocytes, cultured fibroblasts, and fresh or frozen non-fixed tissue specimen. DNA will be isolated (extracted) from the submitted sample(s) and will be stored in the laboratory for future testing. If amniotic fluid sample is submitted, sample will be first processed for amniocyte culture then DNA isolation will be performed on cultured amniocytes; amniotic fluid culture fee and flask charge will apply.

This service should NOT be considered as a DNA banking service; the laboratory reserves the right to discard the stored DNA sample after a certain number of years and will not guarantee the availability of DNA sample beyond that time.

Please click on the Lab Form Link in the Forms Section to print and complete the Genetic Test Requisition Form. Submission of completed Genetic Test Requisition Form is required, and submission of informed consent form is recommended. If testing is desired on the stored DNA sample, please call (614) 722-2866 and ask to speak to a laboratory genetic counselor to coordinate testing.