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Laboratory Test Directory

Culture- Bacterial, Stool Pathogens (Updated)

Components

Name Method Department Units
Culture, Stool Standard microbiologic culture media and techniques
Enzyme immunoassay (EIA)
CMI, Microbiology

Battery Members

Culture for Salmonella and Shigella, Culture for additional pathogen (x4), E. coli Shiga-Toxin EIA

Specimen Requirements

Stool

Container Type Container Size Specimen Volume
Preferred

Cary-Blair transport container

N/A 5 gm or 5 mL
Stability

Room temperature - 24 hour(s)
Refrigerated - 72 hour(s)

Alternate

Dual culture swab system

N/A
Stability

Refrigerated - 24 hour(s)
Room temperature - 24 hour(s)

Alternate

Sterile container

N/A 5 gm or 5 mL
Stability

Room temperature - 2 hour(s)

Specimen Preparation

  • Transport to laboratory as soon as possible
  • Raw stool must be placed into Cary-Blair media within 2 hours of collection

Reasons for Rejection

  • Stool received in O&P kit
  • Stool in diaper
  • Not received within specified time frame
  • Not received at proper temperature
  • Frozen specimen
  • Specimens from inpatients after the third hospital day without prior consultation

Comments

Additional criteria: 

1. Do not accept specimens from inpatients after the third hospital day, without prior consultation. Contact microbiology laboratory 722-5370.

2.It is the recommendation that no more than 2 specimens are collected without prior consultation. Contact microbiology laboratory 722-5370.

Routine stool culture includes screen for Salmonella, Shigella, Campylobacter, Yersinia, Aeromonas, E. coli O157(Enterohemorrhagic E.coli), and EIA for Shiga-toxin producing E. coli. Contact Laboratory Services (614-722-5477 or 800-934-6575) if a special request is needed for Vibrio or other agents. Preliminary results available at 48 hours. Two (2) stool specimens collected on separate days is recommended to increase the detection of bacterial agents of community-acquired gastroenteritis. In patients with persistent diarrhea but negative stool cultures, contact the Clinical Microbiology Lab, 614-722-5370, for additional recommendations.

CPT Code

  • 87045
  • 87046 (x4)
  • 87427