New Search

Laboratory Test Directory

Post-Transplant Analysis, Bone Marrow Engraftment

Components

Name Method Department Units
Bone Marrow Engraftment - Post Transplant Capillary electrophoresis
Polymerase chain reaction (PCR)
Microsatellite marker analysis
Molecular Genetics

Specimen Requirements

Bone marrow, Peripheral blood

Container Type Container Size Specimen Volume
Preferred

Purple tube (EDTA)

4 mL 4 mL
Stability

Room temperature - 24 hour(s)
Refrigerated - 72 hour(s)

Specimen Preparation

  • Do not freeze
  • Do not centrifuge
  • Keep at room temperature

Sorted cells

Container Type Container Size Specimen Volume
Required

Green tube (Sodium heparin), No Gel

6 mL 6 mL
Stability

Room temperature - 24 hour(s)

Specimen Preparation

  • Offsite collections, please contact Send Out Lab (614-722-5396) for special arrangements.
  • For CD3/CD33 cell sorting: Draw anytime Monday-Thursday; Friday draw must be done on main campus and draw before 11:00am,; Do not collect on Friday if collected off main campus
  • Do Not collect on Saturday or Sunday
  • Transport to the lab immediately after collection
  • Specimen must be processed within 24 hours of collection
  • Peripheral blood only, bone marrow not acceptable for sorted cells
  • Do not freeze
  • Do not centrifuge

Reasons for Rejection

  • Centrifuged specimen
  • Clotted specimen
  • Frozen specimen
  • Wrong collection tube

Comments

Submission of completed BMT Requisition Form required - call (614) 722-2866 to obtain the form. In order to perform this test, the recipient and the donor previously must have had a "Bone Marrow Engraftment Pre-Transplant Analysis (Test Code: BMPR)" performed by the laboratory to obtain genotype of the recipient and donor. All samples should be labeled with specimen type (e.g. blood, bone marrow).

This is a DNA-based (molecular genetic) test that quantitatively evaluates polymorphic genetic markers present in the sample. Post-transplant recipient genotypes will be compared to pre-transplant recipient and donor genotypes previously determined by "Bone Marrow Engraftment Pre-Transplant Analysis (Test Code: BMPR)."

Bone marrow engraftment analysis, also known as chimerism testing, involves determining the percentage of donor cells present in the recipient sample after allogeneic bone marrow transplant (BMT) or hematopoietic stem cell transplant (HSCT). Engraftment monitoring after BMT/HSCT is critical to assess the outcome of transplant and to predict the risk of relapse. Quantitative analysis of polymorphic genetic markers, such as this test, has become the standard technique for engraftment analysis due to its high sensitivity.

If analysis of sorted cells (CD3/CD33 or CD3/CD33/CD19/CD56) is requested, please indicate on the lab order/test requisition form the type of cell sorting requested and send the completed Cell Sorting Requisition Form with the sample (complete OSU form for CD3/CD33; Hematologics Lab form for CD3/CD33/CD19/CD56) . Only peripheral blood samples can be used for cell sorting (Bone marrow samples CANNOT be used for cell sorting). Blood samples for cells sorting must be collected in NaHep tubes; a separate EDTA tube blood also needed for whole blood analysis. Minimum of 4 mL NaHep blood is required to perform CD3/CD33 cell sorting, and minimum of 8mL NaHep blood is required to perform CD3/CD33/CD19/CD56 cell sorting (blood preferred to be collected between Monday through Thursday in the early afternoon before 4:00pm).?Also indicate on lab order/test requisition form whether testing is requested on: 1) whole blood AND sorted cells, 2) whole blood ONLY, or 3) sorted cells ONLY. Cell sorting must be performed within 24 hours from blood collection; therefore, avoid blood collection for cell sorting on Friday, Saturday, and Sunday to ensure sufficient sample transport time and cell sorting processing time before the weekend lab closure.

For post-transplant chimerism analysis by XX/XY FISH, please see test code CHIMFISH (Post-Transplant Analysis by FISH).

Please call the Institute for Genetics Medicine Clinical Laboratory at (614) 722-2866 with questions.

CPT Code

  • 81267 (81268 added for additional samples such as sorted cells)