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Laboratory Test Directory

Post-Transplant Analysis, Bone Marrow Engraftment

Components

Name Method Department Units
Bone Marrow Engraftment - Post Transplant Capillary electrophoresis
Polymerase chain reaction (PCR)
Microsatellite marker analysis
Molecular Genetics

Specimen Requirements

Bone marrow, Peripheral blood

Container Type Container Size Specimen Volume
Preferred

Purple tube (EDTA)

4 mL 4 mL
Stability

Room temperature - 24 hour(s)
Refrigerated - 72 hour(s)

Specimen Preparation

  • Do not freeze
  • Do not centrifuge
  • Keep at room temperature

Sorted cells

Container Type Container Size Specimen Volume
Required

Green tube (Sodium heparin), No Gel

6 mL 6 mL
Stability

Room temperature - 24 hour(s)

Specimen Preparation

  • Offsite collections, please contact Send Out Lab (614-722-5396) for special arrangements.
  • For CD3/CD33 cell sorting: Draw anytime Monday-Thursday; Friday draw must be done on main campus and draw before 11:00am,; Do not collect on Friday if collected off main campus
  • Do Not collect on Saturday or Sunday
  • Transport to the lab immediately after collection
  • Specimen must be processed within 24 hours of collection
  • Peripheral blood only, bone marrow not acceptable for sorted cells
  • Do not freeze
  • Do not centrifuge
  • For CD3/CD33/CD19/CD56 cell sorting: Draw between Monday-Friday between 2:00PM-4:00PM

Reasons for Rejection

  • Centrifuged specimen
  • Clotted specimen
  • Frozen specimen
  • Wrong collection tube

Comments

Submission of completed Molecular Genetics BMT Requisition Form required, call (614) 722-2866 to obtain. Must have previously ordered test code BMPR (Bone Marrow Engraftment Pre-Transplant Analysis) in order to perform this test. Please call Molecular Genetics Lab at (614) 722-2866 with questions. For post-transplant chimerism analysis by XX/XY FISH, please see test code CHIMFISH (Post-Transplant Analysis by FISH). All samples should be labeled with specimen type (e.g. blood, bone marrow). Bone marrow engraftment analysis, also known as chimerism testing, involves determining the percentage of donor cells present in the recipient sample after allogeneic bone marrow transplant (BMT) or hematopoietic stem cell transplant (HSCT). Engraftment monitoring after BMT/HSCT is critical to assess the outcome of transplant and to predict the risk of relapse. Quantitative analysis of polymorphic genetic markers has become the standard technique for engraftment analysis due to its high sensitivity. Post-transplantation recipient genotypes will be compared to pre-transplant recipient and donor genotypes previously determined by pre-transplant bone marrow engraftment analysis. If analysis on SORTED CELLS (CD3/CD33 or CD3/CD33/CD19/CD56) is required, please notify the lab and send the completed Cell Sorting Requisition Form (OSU or Hematologics Lab form) with the sample. Only peripheral blood samples can be used for cell sorting and must be collected in NaHep tubes (a separate EDTA tube blood also needed for analysis). Minimum of 4 mL NaHep blood is needed to perform CD3/CD33 cell sorting, and minimum of 8mL NaHep blood is needed to perform CD3/CD33/CD19/CD56 cell sorting. Bone marrow samples cannot be used for cell sorting. Also indicate if testing is needed on sorted cells ONLY, or on a whole peripheral blood sample AND the sorted cells. Cell sorting needs to be performed within 24 hours from blood collection; therefore, avoid blood collection on Friday, Saturday, and Sunday to ensure sufficient sample transport time and cell sorting processing time before the weekend lab closure.

CPT Code

  • 81267 (81268 added for additional samples such as sorted cells)