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Laboratory Test Directory

Culture- Bacterial, Anaerobic


Name Method Department Units
Anaerobe Culture Standard anaerobic culture media and techniques
CMI, Microbiology

Specimen Requirements

Tissue (Fresh), Aspirate, Body fluid

Container Type Container Size Specimen Volume

Sterile container


Room temperature - 2 hour(s)


Anaerobic Transport Medium (Port-a-Cul)


Room temperature - 72 hour(s)

Specimen Preparation

  • Do not refrigerate
  • Transport to laboratory as soon as possible

Reasons for Rejection

  • Non-Port-A-Cul swabs
  • Swab specimen from superficial sites
  • Specimen container with needle attached
  • Not received at proper temperature
  • Not received within specified time frame
  • Wrong type of specimen


An anaerobic culture is recommended with bacterial culture of surgical tissues, biopsy tissues, deep organ abscesses and deep tissue aspirates. If tissue sample is small, add minimal volume of sterile normal saline to prevent dessication. Specimens that contain a large number of normal anaerobic flora are not suitable for anaerobic culture. Unsuitable specimens include: stools, vaginal and cervical swabs, upper respiratory tract, tracheal trap, urine other than suprapubic aspirates, areas obviously contaminated by intestinal contents (such as colostomy stoma) or specimens collected from superficial wounds.

CPT Code

  • 87075