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Laboratory Test Directory

Anti-Nuclear Antibody

Components

Name Method Department Units
ANA (IFA) Immunofluorescent assay
CMI, Immunology Negative or Positive with titer

Specimen Requirements

Whole blood

Container Type Container Size Specimen Volume
Preferred

Red tube, (no anticoagulant), No Gel

3 mL 3 mL
Alternate

Gold tube, (SST) with Gel

3.5 mL 3.5 mL
Stability

Room temperature - 1 hour(s)
Refrigerated - 1 week(s)

Specimen Preparation

  • Collect by venipuncture
  • Allow to clot completely at room temperature
  • Transport to laboratory as soon as possible
  • If delay in transport greater than 1 hour, refrigerate

Serum Spun

Container Type Container Size Specimen Volume
Preferred

Red tube, (no anticoagulant), No Gel

3 mL 3 mL
Alternate

Gold tube, (SST) with Gel

3.5 mL 3.5 mL
Stability

Refrigerated - 1 week(s)
Frozen - 12 month(s)

Specimen Preparation

  • Collect by venipuncture
  • Allow to clot completely at room temperature
  • Transport to laboratory as soon as possible
  • Centrifuge, remove plasma/serum and refrigerate

Reasons for Rejection

  • Wrong collection tube
  • Capillary specimen
  • Gross hemolysis
  • Gross lipemia
  • Bacterial contamination

Comments

Positive screens will be titered (cpt 86039)

CPT Code

  • 86038
  • reflex to 86039 if needed