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Laboratory Test Directory

Amniotic Fluid Cell Culture, Cytogenetics Lab (Updated)

Components

Name Method Department Units
Amniotic Fluid Culture Only, Cytogenetics Lab Cell Culture
Cryopreservation
Cytogenetics

Specimen Requirements

Amniotic fluid

Container Type Container Size Specimen Volume
Preferred

Sterile container

N/A 20 mL-30 mL
Stability

Room temperature - 24 hour(s)

Specimen Preparation

  • Do not add fixative
  • Do not centrifuge
  • Do not freeze
  • Transport to laboratory as soon as possible
  • Keep at room temperature

Reasons for Rejection

  • Delayed or improper handling
  • Frozen specimen
  • Fixed specimen

Comments

Submission of a completed Prenatal Genetic Test Requisition Form is required. To obtain the form, please call Cytogenetics lab at (614) 722-5321.

This test is intended for amniotic fluid samples that does not have any accompanying cytogenetic test order (e.g. chromosome analysis) but cell culture is desired for other molecular genetic or biochemical testing, such as familial mutation analysis, DNA isolation and storage, and send-out testing for molecular genetic/biochemical testing to be done on cultured amniocytes. This test includes cryopreservation of cultured amniocytes.

If additional in-house or send-out testing on cultured amniocytes is desired, please clearly indicate the desired testing on the requisition form and call the Cytogenetics lab at (614) 722-5321 and speak to a laboratory genetic counselor to coordinate. Otherwise, cultured cells will be cryopreserved for future testing. If cultured amniocytes will be used to perform fetal molecular genetic testing, submission of a maternal blood sample (4 mL in EDTA tube) is highly recommended to perform maternal cell contamination study.

CPT Code

  • 88235
  • 88240