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Laboratory Test Directory

ACTH (Adrenocorticotropic Hormone)


Name Method Department Units
ACTH Chemiluminescent Immunoassay
Core Lab, Endocrinology pg/mL

Battery Members


Specimen Requirements

Plasma Spun

Container Type Container Size Specimen Volume

Purple tube (EDTA)

3 mL 1 mL

Purple tube (EDTA)

Micro sample tube 1 mL

Refrigerated - 60 minute(s)
Frozen - 30 day(s)

Specimen Preparation

  • Cool tube in ice before collection
  • Collect by venipuncture
  • Mix EDTA tube by gentle inversion immediately after collection
  • Place on ice immediately after collection
  • Centrifuge within 1 hour, separate plasma and freeze
  • Transport to lab on ice immediately after collection

Reasons for Rejection

  • Delayed or improper handling
  • Frozen whole blood
  • Non EDTA specimen
  • Not centrifuged within time frame
  • Not frozen within specified time frame
  • Not received at proper temperature
  • Not received within specified time frame
  • Not removed from cells within time frame
  • Room temperature receipt
  • Specimen collected in wrong tube type
  • Specimen not kept on ice or refrigerated


Specimen must be maintained on ice (or refrigerated) and centrifuged within 60 minutes of collection. Use EDTA (purple-top tube) plasma only, preferably via venipuncture. Collect the whole blood into iced purple-top (EDTA) tube, noting time of collection. After collection, immediately immerse the tube(s) in an ice bath.

Biotin could cause a falsely elevated or falsely lowered result, but the interference is usually within +/- 20% of the correct value.

Physicians are encouraged to ask patients about their supplement status, especially of biotin, prenatal, and/or hair, nail, and skin supplements. If on high-dose biotin supplementation, it may be necessary to have patients abstain from their supplements for 8-72 hrs prior to blood collection, depending on the dose of biotin taken.

CPT Code

  • 82024