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Laboratory Test Directory

Vitamin D-25 Hydroxy

Components

Name Method Department Units
25-OH Vitamin D Chemiluminescent Microparticle Immunoassay (CMIA)
Core Lab, Endocrinology ng/mL

Specimen Requirements

Whole blood

Container Type Container Size Specimen Volume
Preferred

Gold tube, (SST) with Gel

3.5 mL 2 mL
Alternate

Red tube, (no anticoagulant), No Gel

Micro sample tube 1 mL
Alternate

Green tube (Lithium heparin), with Gel

3 mL 2 mL
Alternate

Green tube (Lithium heparin), No Gel

3 mL 2 mL
Alternate

Green tube (Lithium heparin), with Gel

Micro sample tube 1 mL
Alternate

Green tube (Lithium heparin), No Gel

Micro sample tube 1 mL
Alternate

Red Tube (no anticoagulant) with Gel

3 mL 2 mL
Stability

Room temperature - 3 day(s)
Refrigerated - 12 day(s)

Specimen Preparation

  • Keep refrigerated

Serum Spun, Plasma Spun

Container Type Container Size Specimen Volume
Preferred

Gold tube, (SST) with Gel

3.5 mL 0.5 mL
Alternate

Green tube (Lithium heparin), No Gel

3 mL 0.5 mL
Alternate

Green tube (Lithium heparin), with Gel

3 mL 0.5 mL
Alternate

Red tube, (no anticoagulant), No Gel

Micro sample tube 0.5 mL
Alternate

Green tube (Lithium heparin), No Gel

Micro sample tube 0.5 mL
Alternate

Green tube (Lithium heparin), with Gel

Micro sample tube 0.5 mL
Alternate

Red tube, (no anticoagulant), No Gel

3 mL 0.5 mL
Stability

Room temperature - 3 day(s)
Refrigerated - 12 day(s)
Frozen - 3 month(s)

Specimen Preparation

  • Centrifuge, remove plasma/serum and refrigerate

Reasons for Rejection

  • Wrong collection tube
  • Gross hemolysis
  • Delayed or improper handling

Comments

Vitamin D, 25 Hydroxy is used to diagnose vitamin D deficiency or sufficiency, and to assess nutritional status. If evaluating for rickets or renal failure then refer to test XVDDI (Vitamin D 1, 25 Dihydroxy).

CPT Code

  • 82306