Laboratory Test Directory
Vitamin D-25 Hydroxy
Components
| Name | Method | Department | Units |
|---|---|---|---|
| 25-OH Vitamin D |
Chemiluminescent Microparticle Immunoassay (CMIA) |
Core Lab, Endocrinology | ng/mL |
Specimen Requirements
Whole blood
| Container Type | Container Size | Specimen Volume |
|---|---|---|
PreferredGold tube, (SST) with Gel |
3.5 mL | 2 mL |
AlternateRed tube, (no anticoagulant), No Gel |
Micro sample tube | 1 mL |
AlternateGreen tube (Lithium heparin), with Gel |
3 mL | 2 mL |
AlternateGreen tube (Lithium heparin), No Gel |
3 mL | 2 mL |
AlternateGreen tube (Lithium heparin), with Gel |
Micro sample tube | 1 mL |
AlternateGreen tube (Lithium heparin), No Gel |
Micro sample tube | 1 mL |
AlternateRed Tube (no anticoagulant) with Gel |
3 mL | 2 mL |
Stability
Room temperature - 3 day(s)
Refrigerated - 12 day(s)
Specimen Preparation
- Keep refrigerated
Serum Spun, Plasma Spun
| Container Type | Container Size | Specimen Volume |
|---|---|---|
PreferredGold tube, (SST) with Gel |
3.5 mL | 0.5 mL |
AlternateGreen tube (Lithium heparin), No Gel |
3 mL | 0.5 mL |
AlternateGreen tube (Lithium heparin), with Gel |
3 mL | 0.5 mL |
AlternateRed tube, (no anticoagulant), No Gel |
Micro sample tube | 0.5 mL |
AlternateGreen tube (Lithium heparin), No Gel |
Micro sample tube | 0.5 mL |
AlternateGreen tube (Lithium heparin), with Gel |
Micro sample tube | 0.5 mL |
AlternateRed tube, (no anticoagulant), No Gel |
3 mL | 0.5 mL |
Stability
Room temperature - 3 day(s)
Refrigerated - 12 day(s)
Frozen - 3 month(s)
Specimen Preparation
- Centrifuge, remove plasma/serum and refrigerate
Reasons for Rejection
- Wrong collection tube
- Gross hemolysis
- Delayed or improper handling
Comments
Vitamin D, 25 Hydroxy is used to diagnose vitamin D deficiency or sufficiency, and to assess nutritional status. If evaluating for rickets or renal failure then refer to test XVDDI (Vitamin D 1, 25 Dihydroxy).
CPT Code
- 82306