The Morphology Core Laboratory was established in 1997 and provides services in histology, electron microscopy, and confocal and laser scanning microscopy.
The Histology section of the core provides basic services such as tissue preparation and embedding, paraffin block or frozen tissue sectioning and H&E staining. The core also provides additional services such as direct and indirect immunohistochemical staining, immunofluorescent staining, serial sectioning, RNase free handling, and selected special stains. The core personnel also have extensive experience in tissue microarray construction.
Routine TEM procedures such as fixation, embedding, sectioning, and visualization are available. The core houses a Hitachi S-4800 high resolution Scanning Electron Microscope (SEM). This microscope has magnification from 30x to 800,000x. The core also utilizes a Hitachi H-7650 Transmission Electron Microscope (TEM) with two digital cameras.
The core houses two confocal microscopes. The Zeiss 510 Meta confocal can acquire single and stacked multicolor fluorescence images with 2 internal detectors and 8 detectors on the Meta system. Two fluorescence detectors in the LSM 700 detect up to four color signals in a (quasi-)simultaneous mode. Both confocal scopes use Zen software to acquire images. Other applications include time series image acquisition, co-localization, FRET analysis, ROI bleaching, and orthogonal viewing of images. Both microscopes have four lasers: 405nm, 488nm, 546nm, and 633nm.
Laser Dissection Microscopy
The core also houses a Zeiss LCM PALM Microbeam Laser Dissecting Scope with a pulsed UV laser (337nm) coupled to a Zeiss Axio microscope. The laser is capable of micro-dissection and micro-ablation of small particles with sub micrometer precision. A Leica DMI6000B inverted microscope with an attached environmental chamber is available. This microscope will do live cell imaging, bright field, IF, and time-lapse imaging. There is also a standard Zeiss Axiophot epifluorescent microscope with a Zeiss Axiocam digital camera and Axiovision software.