Gastroenterologists regard direct pancreatic function testing as the gold standard to assess pancreatic function, but anecdotal evidence and recent publications have shown highly variable methods in conducting the tests – especially the relatively easy and safe endoscopic pancreatic function test.
So pediatric gastroenterologists at Nationwide Children’s Hospital and Arnold Palmer Children’s Hospital have developed a recommended technique to ensure accurate assessments.
“Based on the physiology of the pancreatic duct and our own knowledge in conducting these tests, we think this scheme should get optimal results,” says Ivor Hill, MD, section chief of Gastroenterology at Nationwide Children’s and senior author of the paper, published in the Journal of Pediatric Gastroenterology and Nutrition.
Among other points, the protocol includes these recommendations:
- The child should be in the left lateral decubitus position to minimize contamination with gastric fluid
- Before intubation, a bolus of secretin (0.2 mcg/kg) or synthetic CCK octapeptide (0.02 or 0.04 mcg/kg) should be administered
- After intubation, all stomach and proximal duodenum fluid should be aspirated and discarded
- Pancreatic fluid should be collected within ten minutes of secretin administration
- Realize that samples with low pH and protein may be contaminated with gastric or duodenal fluid
These and other suggestions are largely focused on removing risks for contamination or dilution, says Dr. Hill, who is also a professor of Clinical Pediatrics at The Ohio State University College of Medicine. They specify the timing of fluid collection, as well, because of research showing that specimens collected more than ten minutes after secretagogue administration have decreased enzymatic activity.
“We wanted to get this concept out there,” says Dr. Hill. “Our next steps are to get collaborative studies together from institutions who are conducting these tests in a standardized way. If we can validate our method, we’ll have helped make this important tool more accurate.”
““Using this protocol, we are identifying more children with low pancreatic enzyme production than in the past,” she says. “This is important because there are effective treatments that can prevent serious nutritional deficiencies.”
Horvath K, Mehta DI, Hill ID. Endoscopic pancreatic function Test (ePFT) and its indications. Journal of Pediatric Gastroenterology and Nutrition. 2018 Dec 29. [Epub ahead of print]