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Center for Molecular and Human Genetics
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Transgenic and Embryonic Stem Cell Core
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The Transgenic and Embryonic Stem Cell Core is devoted to assisting investigators with all aspects of mouse embryo manipulation, including the design, creation and rederivation of genetically engineered mice. The core provides routine DNA microinjection of zygotes, electroporation of targeting constructs into embryonic stem cells, and the production of chimeras by several different approaches, including blastocyst injection of embryonic stem cells and both embryo-to-embryo and embryo-to-ES cell aggregation.
The core also helps investigators design and construct gene targeting vectors and transgenes. Several mouse lines, including Rosa26, Rosa26reporter, Rosa-Flpe, and Sox2-Cre, and BAC recombineering reagents are available to investigators. The core also offers cryopreservation of morula-stage embryos recovered from mouse strains housed at The Research Institute.
Protocol and additional information are available from The Research Institute at Nationwide Children's Hospital staff.
