Reagents

GSK-3α Plasmids

GSK-3β Plasmids

Methylation-related Plasmids

• Dnmt3a2 pCAGEN
• Dnmt3a2 WT promoter pCR8GW
• Dnmt3a2 NMyc mut promoter pCR8GW
• Dnmt3a2 WT promoter pGLF
• Dnmt3a2 NMyc mut promoter pGLF

Cloning Plasmids

•  pcDNA^TM-DEST47 (C-terminal GFP tag)
• pcDNA^TM-DEST53 (N-terminal GFP tag)
• pDEST^TM26 (N-terminal 6xHis tag)
• pDEST^TM27 (N-terminal GST tag)
• pBAD-DEST49 (Bacterial Expression)
• pcDNA^TM-DEST40 (C-terminal V5 6xHis tag)
• pCAGEN-GW (chicken β-actin promoter, untagged)pCS2+GW (Gateway® Compatible destination vector)
• pMACS Kk II-GW (Gateway® Compatible destination vector for use with Miltenyi Biotec magnetic bead enrichment of transfected mammalian cells)