Protocols

We work with a variety of experimental procedures from southern blotting to mammalian cell culture and have posted here what works for us. Feel free to download and use any of our protocols and don't hesitate to contact us with any questions.

Methylation Analysis:

• gDNA Isolation for Bisulfite Sequencing
• Bisulfite Conversion of Genomic DNA - we have used both the Invitrogen MethylCode^TM Bisulfite Conversion Kit and the Zymo Research EZ DNA Methylation^TM Kit with great success
• Primer Design Strategy for Bisulfite Sequencing
• Sequencing of Bisulfite Converted DNA - coming soon

DNA cloning:

• Invitrogen Gateway cloning- Also see Invitrogen's website for more information
• Mutagenesis

Mouse work:

• Recombineering for Conditional Knockout Mice

Protein:

• Tris-Tricine Gels and Buffer Recipes for Western Blotting
• Western Blotting
• V5 pulldown
• GST pulldown
• Immunofluorescence