The Flaño laboratory studies how the immune system responds to viral infections, with a special emphasis on respiratory virus infections --influenza virus and respiratory syncytial virus-- and persistent herpesvirus infections.
The overall goal of the laboratory is to understand the interplay between the innate and adaptive immune systems, and how they contribute to the generation of long-term protective immunity during infection and vaccination. To this extent, the laboratory studies human clinical samples and mouse models of infection and vaccination using methods such as gene expression analysis and multicolor flow-cytometry.
Flaño's lab has recently shown that airway epithelial cells mount pathogen specific responses and that regulate the function of T cells in the lung through innate interferons and the PD-L1/PD-1 axis. These findings suggest that PDL-1 expression by airway epithelial cells during the early phase of the response to influenza virus infection represents a mechanism for regulating lung immune homeostasis and reveal a promising strategy to modulate local epithelial-T cell interaction in the airways.
McNally B, Ye F, Willette M, Flaño E. Local blockade of epithelial PDL-1 in the airways enhances T cell function and viral clearance during influenza virus infection. Journal of Virology. 2013 Sep 25. [Epub ahead of print]
Bentebibel SE, Lopez S, Obermoser G, Schmitt N, Mueller C, Harrod C, Flaño E, Mejias A, Albrecht RA, Blankenship D, Xu H, Pascual V, Banchereau J, Garcia-Sastre A, Palucka AK, Ramilo O, Ueno H. Induction of ICOS+CXCR3+CXCR5+ TH cells correlates with antibody responses to influenza vaccination. Science Translational Medicine. 2013 Mar 13, 5(176):176ra32.
Cyktor JC, Carruthers B, Stromberg P, Flaño E, Pircher H, Turner J. Killer cell lectin-like receptor G1 deficiency significantly enhances survival after Mycobacterium tuberculosis infection. Infection and Immunity. 2013 Apr, 81(4):1090-9. Epub 2013 Jan 22.
Ioannidis I, Ye F, McNally B, Willette M, Flaño E. Toll-like receptor expression and induction of type I and type III interferons in primary airway epithelial cells. Journal of Virology. 2013 Mar, 87(6):3261-70. Epub 2013 Jan 9.
McNally B, Willette M, Ye F, Partida-Sanchez S, Flaño E. Intranasal administration of dsRNA induces interferon-α receptor-dependent accumulation of antigen experienced T cells in the airways. PLoS One. 2012, 7(12):e51351. Epub 2012 Dec 7.
Ye F, Turner J, Flaño E. Contribution of pulmonary KLRG1 high and KLRG1 low CD8 T cells to effector and memory responses during influenza virus infection. Journal of Immunology. 2012 Dec 1, 189(11):5206-11. Epub 2012 Oct 22.
Aeffner F, Bratasz A, Flaño E, Powell KA, Davis IC. Postinfection A77-1726 treatment improves cardiopulmonary function in H1N1 influenza-infected mice. American Journal of Respiratory Cell and Molecular Biology. 2012 Oct, 47(4):543-51. Epub 2012 Jun 7.
Ioannidis I, McNally B, Willette M, Peeples ME, Chaussabel D, Durbin JE, Ramilo O, Mejias A, Flaño E. Plasticity and virus specificity of the airway epithelial cell immune response during respiratory virus infection. Journal of Virology. 2012 May, 86(10):5422-36. Epub 2012 Mar 7.