Maqsood Chotani, PhD

Maqsood A. Chotani, PhD, is Principal Investigator at the Center for Cardiovascular & Pulmonary Research, Nationwide Children’s Hospital, and Assistant Professor, Department of Pediatrics, The Ohio State University Medical Center.

View CV »

Gender:

• Male

Languages Spoken:

• English

Research Center:

• Center for Cardiovascular and Pulmonary Research

Areas of Interest:

• Project 2: The second messenger cyclic AMP- and cooling-coupled signature molecules in micro-vascular smooth muscle cells This project includes examination of signature microRNAs and proteins in human vascular smooth muscle cells utilizing chip/PCR analyses and 2D-proteomics (fluorescence difference gel electrophoresis), respectively. This approach will potentially lead to identification of physiologically relevant "vasculo-protective" players (or markers), conferring "stress-tolerance" to cells during cooling or vascular injury. Preliminary studies have identified specific proteins and microRNAs altered by cyclic AMP and by cooling.
• Project 3: Role of the Ras-related small GTPase Rap1A in the cardiovasculature Rap1 acts as a molecular switch, coupling extracellular stimulation to intracellular signaling through the second messenger cyclic AMP. The key objective of this project is to examine the role of this protein in cell attachment and cell survival in vascular smooth muscle cells, and in cardiac myocyte function. Preliminary studies using a live animal model (with genetic ablation of Rap1 subtype A) suggest that Rap1A has a vital role in maintaining normal cardiac structure and function. Studies are underway to elucidate the molecular mechanism(s) of Rap1A signaling and function. We are also using intact blood vessels and explanted smooth muscle cells from blood vessels of wild-type and Rap1A-deficient mice to study the role of this GTPase in the vasculature.
• My research interests and activities are currently supported by Institutional internal funds, the American Heart Association, and the National Heart, Lung, and Blood Institute of the National Institutes of Health. The funds support research examining mechanisms of expression and cell-surface mobilization of endogenous micro-vascular smooth muscle G protein-coupled a2C-adrenergic receptors (a2C-ARs). My colleagues and I identified the a2C-ARs as “stress-receptors” of the vascular sympathetic system. We have recently identified a novel signaling cascade in human vascular smooth muscle cells involving cyclic AMP, the Ras-related small G protein Rap1, actin binding protein filamin2, a2C-ARs, and the actin cytoskeleton. Current and future work involves studies to understand the role of Rap1 in the cardiovasculature and examination of stress-induced signature microRNAs and proteins in vascular smooth muscle cells. These studies will potentially lead to identification of physiologically relevant "vasculo-protective" players, conferring "stress-tolerance" to cells during cellular stress or vascular injury. Broadly, these studies involve understanding the mechanisms behind alterations in peripheral vascular resistance, and peripheral vascular disorders. For example, in the cerebral circulation, in stroke, diabetes, hypercholesterolemia, hypertension, hypothermic response of the microvasculature seen in Raynaud’s phenomenon, secondary Raynaud’s associated with the devastating disease scleroderma, and hand-arm vibration syndrome.
• Project 1: Mechanisms of expression and mobilization of micro-vascular smooth muscle a2C-adrenoceptors (a2C-ARs) The key objective of this project is to understand the biology of the G protein coupled a2- adrenoceptor subtype C (a2C). This receptor has been implicated in the cold-triggered spasms in blood vessels of individuals suffering from Raynaud’s phenomenon, secondary Raynaud’s associated with scleroderma, and hand-arm vibration syndrome. A unique feature of a2C-AR regulation includes intracellular retention. The receptor translocates to the plasma membrane under select physiological conditions of vascular stress, engaging with the agonist norephinephrine, and eliciting a biological response of vasoconstriction. Our recent work has uncovered an essential role of the cytoskeleton in receptor mobilization to the cell surface. We are developing a peptide decoy that may reduce cell surface receptor mobilization, and may have therapeutic potential.

Undergraduate

• The College of Wooster
  Date Completed: 06/30/1988

Post Doctoral

• The Ohio State University
  Date Completed: 06/30/1997

Fellowship

• The Ohio State University, Dept. of Internal Medicine
  Date Completed: 06/30/1998

Fellowship

• Davis Heart & Lung Research Institute
  Date Completed: 06/30/2002

Section:

• Center for Cardiovascular and Pulmonary Research

2008–present

• Principal Investigator, Center for Cardiovascular and Pulmonary Research, The Research Institute at Nationwide Children’s Hospital, Columbus, Ohio

2002–2008

• Research Scientist, Davis Heart & Lung Research Institute, The Ohio State University

1998–2002

• Postdoctoral Researcher/Fellow, Davis Heart & Lung Research Institute, The Ohio State University

1997–1998

• Postdoctoral Researcher, Department of Internal Medicine, The Ohio State University

1990–1997

• Graduate Research Associate, Program in Molecular, Cellular & Developmental Biology, The Ohio State University, Columbus, Ohio

1988–1990

• Jr. Research Associate, Department of Psychiatry, MetroHealth Medical Center, Cleveland, Ohio

1988–1988

• Laboratory Technician, Department of Preventive Veterinary Medicine, Food Animal Health Research Program, Ohio Agriculture Research & Development Center, Wooster, Ohio

• Jeyaraj SC, Unger NT, Chotani MA. 2011. Rap1 GTPases: an emerging role in the cardiovasculature.  Life Sciences (Molecular, Cellular and Functional Basis of Therapy). Vol. 88, no. 15-16. (April 11): 645-652. (IF: 2.672)
• Chotani MA; Flavahan NA. 2011. Intracellular alpha2C-adrenoceptors: storage depot, stunted development or signaling domain?.  Biochimica et Biophysica Acta-Molecular Cell Research. Vol. doi: 10.1016/j.bbamcr.2011.05.006. (IF: 5.275) (Formally Accepted)
• Jeyaraj SC, Unger NT, El-Dahdah NP, Sessler RJ, Zirwas M, Pleister A, Green-Church KB, McConnell B, Chotani MA. 2010. Delocalization of endogenous human microvascular smooth muscle A-kinase antagonizes the positive effects of Rap-Rho signaling on cell integrity and survival. [Abstract]. Circulation. Vol. 122, no. 21 Supplement: American Heart Association. (November 13) (IF: 14.049) [Peer Reviewed] (Published)
• Jeyaraj SC, Unger NT, El-Dahdah NP, Sessler RJ, Zirwas M, Pleister A, Green-Church KB, Chotani MA. 2010. Analysis of cyclic AMP-coupled signature molecules in human micro vascular smooth muscle cells shows link to stress-associated proteins. [Abstract]. FASEB J.. Vol. 24: 870.1. (IF: 7.096) (Published)
• Jeyaraj SC, Joshi MS, Huang H, Unger NT, El-Dahdah NP, Wold LE, Strauch AR, Elton TS, Quilliam LA, Bauer JA, Chotani MA. 2010. Electrophysiological abnormalities in mice with genetic ablation of Rap1a GTPase. [Abstract]. FASEB J.. Vol. 24: 867.3. (IF: 7.096) (Published)
• Mihai C; Chotani M; Elton TS; Agarwal G. 2009. Mapping of DDR1 distribution and oligomerization on the cell surface by FRET microscopy.  Journal Of Molecular Biology. Vol. 385, no. 2. (January 16): 432-445. (IF: 4.303)
• Mihai C, Chotani M, Elton TS, Agarwal G. 2009. Mapping of DDR1 distribution and oligomerization on the cell surface by FRET microscopy.  J. Mol. Biol.. Vol. 385: 432-445.
• Eid AH; Chotani MA; Mitra S; Miller TJ; Flavahan NA. 2008. Cyclic AMP acts through Rap1 and JNK signaling to increase expression of cutaneous smooth muscle alpha2C-adrenoceptors.  American Journal Of Physiology. Heart And Circulatory Physiology. Vol. 295, no. 1. (July 1): H266.
• Eid AH?, Chotani MA?, Mitra S, Miller TJ, Flavahan NA. 2008. Cyclic AMP acts through Rap1 and JNK signaling to increase expression of cutaneous smooth muscle ?2C-adrenoceptors.  Am J Physiol. Heart Circ Physiol. Vol. 295. : H266-H272.
• Moustafa-Bayoumi M; Alhaj MA; El-Sayed O; Wisel S; Chotani MA; Abouelnaga ZA; Hassona MD; Rigatto K; Morris M; Nuovo G; Zweier JL; Goldschmidt-Clermont P; Hassanain H. 2007. Vascular hypertrophy and hypertension caused by transgenic overexpression of profilin 1.  The Journal Of Biological Chemistry. Vol. 282, no. 52 (December 28): 37632.
• Eid AH; Maiti K; Mitra S; Chotani MA; Flavahan S; Bailey SR; Thompson-Torgerson CS; Flavahan NA. 2007. Estrogen increases smooth muscle expression of alpha2C-adrenoceptors and cold-induced constriction of cutaneous arteries.  American Journal Of Physiology. Heart And Circulatory Physiology. Vol. 293, no. 3 (September 1): H1955.
• Chotani, MA; Eid, AH; Mitra, S; Flavahan, NA. 2007. An arginine-rich region mediates transGolgi retention of alpha2C-adrenoceptors. [Abstract]. FASEB J. Vol. 21, no. 6. (April): A1210. (IF: 7.096) (Published)
• Martin MM, Buckenberger JA, Jiang J, Malana GE, Nuovo GJ, Chotani M, Feldman DS,Schmittgen TD, Elton TS. 2007. The human angiotensin II type 1 receptor +1166 A/C polymorphism attenuates microRNA-155 binding.  Journal of Biological Chemistry. Vol. 282, no. 33. : 24262-24269. (IF: 5.44)
• Bayoumi MM, Alkheraije KA, El-Sayed O, Wisel S, Sarker K, Chotani MA, Zweier JL, Nuovo G, Goldschmidt-Clermont PJ, Hassanain H. 2007. Vascular hypertrophy caused by transgenic overexpression of profilin 1.  J. Biol. Chem.. Vol. 282. : 37632-37639.
• Eid A H, Maiti K, Mitra S, Chotani MA, Flavahan S, Bailey SR, Thompson-Torgerson CS, Flavahan NA. 2007. Estrogen increases smooth muscle expression of ?2C-adrenoceptors and cold-induced constriction of cutaneous arteries.  Am J Physiol. Heart Circ Physiol.. Vol. 293. : H1955-H1961.
• Chotani, MA; Mitra, S; Eid, AH; Flavahan, NA. 2007. Rap1 and filamin-2 regulate subtype-specific, cell-surface delivery of alpha2-adrenoceptors [Abstract]. FASEB J. Vol. 21, no. 6: A1209. (IF: 7.096) (Published)
• Chotani, MA; Mitra, S; Flavahan, NA. 2006. Interaction with filamin2 enables translocation of alpha2C-andrenoceptors to the plasma membrane of smooth muscle cells [Abstract]. FASEB J. Vol. 20, no. 5: A1176. (IF: 7.096) (Published)
• Chotani, MA; Mitra, S; Eid, AH; Miller, TJ; Flavahan, NA. 2006. Rap1 GTPase stimulates translocation of alpha2C-adrenoceptors from the transGolgi to the plasma membrane [Abstract]. FASEB J. Vol. 20, no. 5: A1177. (IF: 7.096) (Published)
• Chotani MA; Mitra S; Eid AH; Han SA; Flavahan NA. 2005. Distinct cAMP signaling pathways differentially regulate alpha2C-adrenoceptor expression: role in serum induction in human arteriolar smooth muscle cells.  American Journal Of Physiology. Heart And Circulatory Physiology. Vol. 288, no. 1. (January 1): H69-H76. (IF: 3.75)
• Eid, AH; Mitra, S; Chotani, MA; Miller, TJ. 2005. A novel Rap1 signaling pathway mediates increased expression of alpha2C-adrenoceptors in human cutaneous vascular smooth muscle cells [Abstract]. Circulation. Vol. (Supplement, October 25, 2005), no. 112: II-131. (IF: 14.049) [Peer Reviewed] (Published)
• Chotani MA; Mitra S; Su BY; Flavahan S; Eid AH; Clark KR; Montague CR; Paris H; Handy DE; Flavahan NA. 2004. Regulation of alpha2-adrenoceptors in human vascular smooth muscle cells.  American Journal Of Physiology. Heart And Circulatory Physiology. Vol. 286, no. 1. (January 1): H59-H67. (IF: 3.75)
• Flavahan NA; Flavahan S; Mitra S; Chotani MA. 2003. The vasculopathy of Raynaud's phenomenon and scleroderma.  Rheumatic Diseases Clinics Of North America. Vol. 29, no. 2. (May 1): 275-291. (IF: 2.351)
• Chotani, MA; Mitra, S; Flavahan, S; Flavahan, NA. 2003. Distinct cyclic AMP signaling pathways differentially regulate alpha2C-adrenoceptor expression in human arteriolar vascular smooth muscle cells [Abstract]. Circulation. Vol. (Supplement IV) Oct 28, 2003, no. 108: 100. (IF: 14.049) [Peer Reviewed] (Published)
• Chotani, MA; Jeyaraj, SC; Mitra, S; Morrison, KJ; Flavahan, S; Flavahan, NA. 2003. Induction of alpha2C-adrenoceptors in human microvascular smooth muscle cells by cyclic AMP dependent, but A-kinase independent signaling [Abstract]. FASEB J. Vol. 17, no. 4: A214. (IF: 7.096) (Published)
• Jeyaraj SC; Chotani MA; Mitra S; Gregg HE; Flavahan NA; Morrison KJ. 2001. Cooling evokes redistribution of alpha2C-adrenoceptors from Golgi to plasma membrane in transfected human embryonic kidney 293 cells.  Molecular Pharmacology. Vol. 60, no. 6. (December 1): 1195-1200. (IF: 4.684)
• Su B; Mitra S; Gregg H; Flavahan S; Chotani MA; Clark KR; Goldschmidt-Clermont PJ; Flavahan NA. 2001. Redox regulation of vascular smooth muscle cell differentiation.  Circulation Research. Vol. 89, no. 1. (July 6): 39-46. (IF: 9.857)
• Jeyaraj, SC; Chotani, MA; Mitra, S; Flavahan, S; Gregg, HE; Flavahan, NA; Morrison, KJ. 2001. Cold-induced redistribution of alpha2C-adrenergic receptors [Abstract]. FASEB J. Vol. 15, no. 4: A217. (IF: 7.096) (Published)
• Jeyaraj, SC; Chotani, MA; Mitra, S; Flavahan, S; Flavahan, NA; Morrison, KJ. 2001. Cooling induces redistibution of alpha2C-adrenergic receptors in HEK293 cells [Abstract]. J Vas Res. Vol. 38, no. (S1): 14. (IF: 2.634) (Published)
• Chotani MA; Touhalisky K; Chiu IM. 2000. The small GTPases Ras, Rac, and Cdc42 transcriptionally regulate expression of human fibroblast growth factor 1.  The Journal Of Biological Chemistry. Vol. 275, no. 39. (September 29): 30432-30438. (IF: 5.44)
• Chotani MA; Flavahan S; Mitra S; Daunt D; Flavahan NA. 2000. Silent alpha(2C)-adrenergic receptors enable cold-induced vasoconstriction in cutaneous arteries.  American Journal Of Physiology. Heart And Circulatory Physiology. Vol. 278, no. 4. (April 1): H1075-H1083. (IF: 3.75)
• Morrison, KJ; Chotani, MA; Flavahan, S; Mitra, S; Gregg, HE; Duant, DA; Flavahan, NA. 2000. Alpha2C-adrenoceptors and cold-induced vasospasm [Abstract]. FASEB J. Vol. 14, no. 8: A1315, May 11, 2000, abstract 27. (IF: 7.096) (Published)
• Chotani, MA; Mitra, S; Su, BY; Flavahan, S; Paris, H; Daunt, DA; Handy, DE; Clark, KR; Flavahan, NA. 2000. Regulation of alpha2C-adrenergic receptor expression in vasular smooth muscle cells [Abstract]. FASEB J. Vol. 14, no. 8: A1315. (IF: 7.096) (Published)
• Chotani, MA; Mitra, S; Su, BY; Flavahan, S; Paris, H; Daunt, DA; Handy, DE; Flavahan, NA. 1999. Microvascular smooth-muscle specific expression of alpha2C-adrenergic receptor: role of oxidant stress and stress-activated protein kinase p38 in gene regulation [Abstract]. Circulation. Vol. (Supplement) 100, no. 18: I-848, November 2, 1999, abstract 4479. (IF: 14.049) [Peer Reviewed] (Published)
• Chotani, MA; Flavahan, S; Mitra, S; Daunt, DA; Flavahan, NA. 1999. Silent alpha2C-adrenergic receptors enable cold-induced vasoconstriction in cutaneous arteries: a mechanism for Raynaud's Phenomenon? [Abstract]. Circulation. Vol. (Supplement) 100, no. 18: I-554, November 2, 1999, abstract 2922. (IF: 14.049) [Peer Reviewed] (Published)
• Payson RA; Chotani MA; Chiu IM. 1998. Regulation of a promoter of the fibroblast growth factor 1 gene in prostate and breast cancer cells.  The Journal Of Steroid Biochemistry And Molecular Biology. Vol. 66, no. 3. (August 1): 93-103. (IF: 2.86)
• Chotani MA; Chiu IM. 1997. Differential regulation of human fibroblast growth factor 1 transcripts provides a distinct mechanism of cell-specific growth factor expression.  Cell Growth & Differentiation: The Molecular Biology Journal Of The American Association For Cancer Research. Vol. 8, no. 9. (September 1): 999-1013. (IF: 4.384)
• Chotani MA; Chiu IM. 1996. A recombinant PCR approach requiring only three non-chimeric primers to generate a minigene of interest.  Genetic Analysis: Biomolecular Engineering. Vol. 12, no. 3-4. (January 1): 133-135.
• Myers RL; Ray SK; Eldridge R; Chotani MA; Chiu IM. 1995. Functional characterization of the brain-specific FGF-1 promoter FGF-1.B.  The Journal Of Biological Chemistry. Vol. 270, no. 14. (April 7): 8257-8266. (IF: 5.44)
• Chotani MA; Payson RA; Winkles JA; Chiu IM. 1995. Human fibroblast growth factor 1 gene expression in vascular smooth muscle cells is modulated via an alternate promoter in response to serum and phorbol ester.  Nucleic Acids Research. Vol. 23, no. 3. (February 11): 434-441. (IF: 7.3)
• Chotani, MA; Winkles, JA; Chiu, I-M. 1994. Promotor switching of FGF-1 gene expression in vascular smooth muscle cells in response to serum and phorbol ester [Abstract]. J Cell Biochem. Vol. Supplement 18A, no. abstract E203: 277. (IF: 3.285) (Published)
• Myers, RL; Chotani, MA; Chiu, I-M. 1994. Transcriptional regulation of human FGF-1: potential interaction between positive and negative regulatory proteins [Abstract]. J Cell Biochem. Vol. Supplement 18A, no. Abstract EZ206: 324. (IF: 3.285) (Published)
• Payson RA; Canatan H; Chotani MA; Wang WP; Harris SE; Myers RL; Chiu IM. 1993. Cloning of two novel forms of human acidic fibroblast growth factor (aFGF) mRNA.  Nucleic Acids Research. Vol. 21, no. 3. (February 11): 489-495. (IF: 7.3)
• Myers RL; Payson RA; Chotani MA; Deaven LL; Chiu IM. 1993. Gene structure and differential expression of acidic fibroblast growth factor mRNA: identification and distribution of four different transcripts.  Oncogene. Vol. 8, no. 2. (February 1): 341-349. (IF: 6.73)
• Piletz JE; Sarasua M; Whitehouse P; Chotani M. 1991. Intracellular membranes are more fluid in platelets of Alzheimer's disease patients.  Neurobiology Of Aging. Vol. 12, no. 5. (September 1): 401-406. (IF: 6.239)
• Piletz JE; Sarasua M; Chotani M; Saran A; Halaris A. 1991. Relationship between membrane fluidity and adrenoceptor binding in depression.  Psychiatry Research. Vol. 38, no. 1. (July 1): 1-12. (IF: 2.766)
• Hodgson CP; Fisk RZ; Arora P; Chotani M. 1990. Nucleotide sequence of mouse virus-like (VL30) retrotransposon BVL-1.  Nucleic Acids Research. Vol. 18, no. 3. (February 11): 673. (IF: 7.3)
• Hodgson, CP; Fisk, RZ; Hatzoglu, M; Arora, P; Chotani, MA; Hanson, RW. 1989. Mouse retrovirus-like (VL-30) transposable element RNA is abundantly expressed in psi-3 helper cells, transferred to recipient rat hepatoma cells, and expressed in dexamethasone-inducible fashion along w/specifically vectored sequences: complete structure [Abstract]. J Cell Biochem. Vol. Supplement 13D, no. abstract K415 (IF: 3.285) (Published)
• Chotani, MA; Mitra, S; Su, BY; Flavahan, S; Paris, H; Daunt, DA; Handy, DE; Flavahan, NA, Presenter. 1999. Microvascular smooth-muscle specific expression of alpha2C-adrenergic receptor: role oxident stress and stress-activated protein kinase p38 in gene regulation. Presented at The American Heart Association Meeting. 72nd Scientific Sessions. Atlanta, GA. (November)
• NA Flavahan; S Flavahan; MA Chotani; S Mitra; B Su, Co-Discoverer. filed June 9, 2000 and issued September 3, 2002. Methods and Compositions for Treating Raynaud's Phenomenon and Scleroderma. patent no. 6,444,681. The Ohio State University.
• Chotani, MA; Winkles, JA; Chiu, I-M, Poster Presenter. 1993. Identification and distribution of a novel human acidic fibroblast growth factor mRNA: a fourth variant having alternative 5' untranslated exon. Presented at Poster Session for the 19th Annual ICSABER Society Graduate Research Forum. The Ohio State University. Columbus, OH. (May)
• Chiu, I-M; Myers, RL; Payson, RA; Chotani, MA, Presenter. 1993. Differential expression of acidic fibroblast growth factors mRNA: gene structure and distribution of four different aFGF transcripts. Presented at Poster Session for Keystone Symposia on Extracellular Matrix in Development and Disease. Breckenridge, CO. (March)
• Chotani, MA, Presenter. 1994. Some aspects of FGF-1 gene expression. Presented at Sixth Annual Ohio State University Molecular Biology Meeting. Stone Laboratory. Gibralter Island, OH. (September)
• Mihai, C; Chotani, MA; Elton, TS; Agarwal, G, Presenter. 2007. Does collagen type 1 modulate oligomerization of DDR1? Presented at 51st Biophysical Society Meeting. Baltimore, MD. (March)
• Chotani, MA, Presenter. 1994. Gene expression of human fibroblast growth factor-1 (FGF-1) is modulated by alternate promotors in response to serum, PMA. and TGF-beta1. Presented at The Scott Falkenthal Memorial Graduate Student Colloquium. Department of Molecular Genetics, The Ohio State University. Columbus, OH. (January)
• Su, BY; Mitra, S; Gregg, H; Flavahan, S; Chotani, MA; Baldwin, WM; Goldschmidt-Clermont, PJ; Flavahan, NA, Presenter. 2000. Regulation of expression of the proapoptotic protein, BAD, by phenotypic modulation of vascular smooth muscle cells. Presented at First Conference on Arteriosclerosis, Thrombosis, and Vascular Biology. Denver, CO. (May)
• Chotani, MA, Presenter. 1995. Heparin and oncogenic ras modulate gene expression of fibroblast growth factor-1: towards an understanding of the biological significance of alternate promoter usage by a growth factor gene. Presented at The Scott Falkenthal Memorial Graduate Student Colloquium. The Department of Molecular Genetics, The Ohio State University. Columbus, OH. (January)
• Chotani, MA; Mitra, S; Flavahan, NA, Presenter. 2006. Interaction with filamin2 enables translocation of alpha 2C-adrenoceptors ot the plasma membrane of smooth muscle cells. Presented at Experimental Biology 2006. San Francisco, CA. (April)
• Chotani, MA, Seminar Presenter. 2003. Expression of alpha2-adrenoceptors in human vascular smooth muscle cells. Presented at The Aga Khan University College of Medicine, to Faculty, staff, medical students. The Department of Biological and Biomedical Sciences. Karachi, Pakistan. (August)
• Savla, M; Sun, P; Rathman, J; Chotani, MA; Agarwal, G, Presenter. 2005. DIP pen nanolithography: a novel approach to study lipid-protein interaction. Presented at 4th Annual Ohio State University Medical Center Graduate & Postgraduate Research Day. Columbus, OH. (March)
• Mihai, C; Vikram, DS; Chotani, MA; Agarwal, G, Presenter. 2004. Does glycosylation mediate discoidin domain receptor (DDR)-collagen interaction? Presented at 3rd Annual Ohio State University Medical Center Graduate & Postgraduate Research Day. Columbus, OH. (April)
• Chotani, MA, Seminar Presenter. 1995. Gene regulation of human fibroblast growth factor-1. Presented at The Clayton Foundation Laboratories for Peptide Biology, to Faculty, staff, students. The Salk Institute for Biological Studies. La Jolla, CA. (August)
• Chotani, MA; Mitra, S; Flavahan, S; Flavahan, NA, Presenter. 2003. Distinct cyclic AMP signaling pathways differentially regulate alpha2C-adrenoceptor expression in human arteriolar vascular smooth muscle cells. Presented at American Heart Association Meeting. Scientific Sessions 2003. Orlando, FL. (November)
• Chotani, MA; Jeyaraj, SC; Mitra, S; Morrison, KJ; Flavahan, S; Flavahan, NA, Presenter. 2003. Induction of alpha2C-adrenoceptors in human microvascular smooth muscle cells by cyclic AMP dependent, but A-kinase independent signaling. Presented at Experimental Biology 2003. American Society for Pharmacology & Experimental Therapeutics. San Diego, CA. (April)
• Su, BY; Mitra, S; Gregg, H; Flavahan, S; Chotani, MA; Goldschmidt-Clermont, PJ; Flavahan, NA, Presenter. 2000. Redox regulation of vascular smooth muscle differentiation. Presented at First Conference on Arteriosclerosis, Thrombosis, and Vascular Biology. Denver, CO. (May)
• Joshi, MS; Bauer, JA; Wold, LE; Strauch, AR; Elton, TS; Quilliam, LA; Chotani, MA, Poster Presenter. 2008. Cardiac abnormalities in mice with genetic ablation of Rap1A GTPase (Award winning poster). Presented at Research Retreat Abstract Competition. Davis Heart & Lung Research Institute, The Ohio State University College of Medicine. Columbus, OH. (October 21)
• Chotani, MA; Chiu, I-M, Presenter. 1997. Cellular and molecular analysis of regulatory mechanisms of human fibroblast growth factor 1 gene expression. Presented at 23rd Annual ICSABER Society Graduate Research Forum. The Ohio State University. Columbus, OH. (May)
• Morrison, KJ; Chotani, MA; Flavahan, S; Mitra, S; Gregg, HE; Daunt, DE; Flavahan, NA, Presenter. 2000. Alpha2C-adrenoceptors and cold-induced vasospasm. Presented at ASBMB/ASPET 2000 Meeting. Boston, MA. (May)
• Eid, A; Mitra, S; Chotani, MA; Flavahan, S; Flavahan, NA, Presenter. 2005. Estrogen increases alpha2C-adrenoceptor expression in human microvascular smooth muscle cells. Presented at Experimental Biology 2005. (April)
• Chotani, MA; Chiu, I-M, Presenter. 1997. Functional analysis of human fibroblast growth factor-1 promoters shows linkage to Ras, Rac1, and Cdc42 signaling. Presented at 9th Annual Ohio State University Molecular Biology Program Stone Laboratory Meeting. Gibralter Island, OH. (September)
• Eid, A; Mitra, S; Chotani, MA; Miller, TJ; Flavahan, NA, Presenter. 2005. A novel Rap1 signaling pathway mediates increased expression of alpha2C-adrenoceptors in human cutaneous vascular smooth muscle cells. Presented at American Heart Association Meeting. Scientific Sessions 2005. Dallas, TX. (November)
• Chotani, MA, Seminar Presenter. 2007. Rap1 and filamin-2 regulate sub-type specific, cell surface delivery of alpha2-adrenoceptors. Presented at Experimental Biology 2007 Symposium, to Session attendees. Featured Topic Session on Receptor Biology: Intracellular Signaling and Second Messengers. Washington, DC. (April)
• Chotani, MA, Seminar Presenter. 2009. Regulated cell surface delivery of alpha2C-adrenoceptors (CME approved presentation). Presented at Research Day, to Session attendees. The Research Institute at Nationwide Children's Hospital. Columbus, OH. (May 15)
• Piletz, JE; Sarasua, MM; Chotani, MA. 1990. Higher platelet internal membrane fluidity in Alzheimer's patients versus controls. Presented at Second International Conference on Alzheimer's Disease and Related Disorders. Toronto, Canada. (July)
• MA Chotani, JA Bauer, MS Joshi, SC Jeyaraj, Co-discoverer. filed April 27, 2011. (patent pending) Rap1A as a marker for cardiac arrhythmia. patent no. Provisional. The Research Institute at Nationwide Children's Hospital. Disc. Date: January 2011.
• Chotani, MA, Presenter. 1995. Transforming growth factor-beta1 and heparin modulate gene expression of fibroblast growth factor-1: towards an understanding of the biological significance of alternate promotor usage by a growth factor gene. Presented at Graduate Research Forum. The Ohio State University. Columbus, OH. (April)
• Myers, RL; Payson, RA; Chotani, MA; Chui, I-M, Presenter. 1992. Tissue specific expression of multiple aFGF transcripts containing different 5' untranslated exons. Presented at Poster Session for Meeting of the American Society of Biochemistry, Molecular Biology and Biophysics. Houston, TX. (February)
• Payson, RA; Chotani, MA; Myers, RL; Harris, SE; Chiu, I-M, Presenter. 1993. Alternate 5' untranslated exon/promotor usage renders tissue-specific transcriptional regulation of the human acidic fibroblast growth factor gene (aFGF). Presented at Poster Session for Keystone Symposia on Transcription: Factors, Regulation and Differentiation. Keystone, CO. (January)
• Chotani, MA, Mitra S, Eid AH, Miller TJ, Flavahan NA, Presenter. 2006. Rap1 GTPase stimulates translocation of alpha2C-adrenoceptors from the transGolgi to the plasma membrane. Presented at Experimental Biology 2006. San Francisco, CA. (April)
• Chotani, MA, Presenter. 1992. Characterization of a novel form of human aFGF mRNA. Presented at Fourth Annual DNA Virus/Gene Expression Meeting. Stone Laboratory, The Ohio State University. Gilbrater Island, OH. (September)
• Huang, H; Joshi, MS; Bauer, JA; Wold, LE; Strauch, AR; Elton, TS; Quilliam, LA; Chotani, MA, Presenter. 2009. Cardiac abnormalities in mice with genetic ablation of Rap1A GTPase (Oral competition finalist). Presented at Research Day. The Research Institute at Nationwide Children's Hospital. Columbus, OH.
• Chotani, MA; Flavahan, S; Mitra, S; Daunt, DA; Flavahan, NA, Presenter. 1999. Silent alpha2C-adrenegic receptors enable cold-induced vasoconstriction in cutaneous arteries: a mechanism of Reynaud's Phenomenon? Presented at The American Heart Association Meeting. 72nd Scientific Sessions. Atlanta, GA. (November)
• Chotani, MA; Jeyaraj, S; Mitra, S; Morrison, KJ; Flavahan, S; Flavahan, NA, Presenter. 2002. Role of COX-2 and cylic AMP in regulating expression of alpha2C-adrenoceptors in human microvascular smooth muscle cells. Presented at 10th International Symposium on Vascular Neuroeffector Mechanisms. Granlibakken, Lake Tahoe, CA. (July)
• Myers, RL; Chotani, MA; Chiu, I-M, Poster Presenter. 1994. Transcriptional regulation of human FGF-1: potential interaction between positive and negative regulatory proteins. Presented at Keystone Symposia on Molecular Biology of the Endothelial Cell. Keystone, CO. (January)
• Chotani, MA; Winkles, JA; Chiu, I-M, Poster Presenter. 1994. Promotor switching of FGF-1 gene expression in vascular smooth muscle cells in response to serum and phorbol ester. Presented at Keystone Symposia on Inflammation, Growth Regulatory Molecules and Atherosclerosis. Keystone, CO. (January)
• Chotani, MA; Chiu, I-M, Poster Presenter. 1994. Increased levels of FGF-1 mRNA in response to serum, phorbol ester, and TGF-beta1: identification of variant transcripts arising from alternate promotors. Presented at Poster Session for the 20th Annual ICSABER Society Graduate Research Forum. The Ohio State University. Columbus, OH. (May)
• Eid, AH; Chotani, MA; Mitra, S; Flavahan, S; Flavahan, NA, Presenter. 2004. Estrogen increases alpha2C-adrenoceptor expression in human microvascular smooth muscle cells. Presented at 3rd Annual Ohio State University Medical Center Graduate & Postgraduate Research Day. Columbus, OH. (April)
• Chotani, MA, Seminar Presenter. 1997. Cellular and molecular analysis of regulatory mechanisms of human Fibroblast Growth Factor 1 gene expression. Presented at Dissertation. The Ohio State University. Columbus, OH.
• Chotani, MA, Seminar Presenter. 2009. Vascular alpha 2C-adrenoceptors: from skin punch biopsy to the culture dish. 2nd International Workshop-cum-Training Course on Molecular Medicine and Drug Research. Karachi, Pakistan. (January)
• Hodgson, CP; Fisk, RZ; Hatzoglu, M; Arora, P; Chotani, MA; Hanson, RW, Poster Presenter. 1989. Mouse retovirus-like (VL-30) transposable element RNA is abundantly expressed in psi-2 helper cells, transferred to recipient rat hepatoma cells, and expressed in a dexamethasone-inducible fashion along with specifically vectored sequences: complete structure. Presented at Poster Session for 18th Annual UCLA Symposia on Biotechnology and Human Genetic Predisposition to Disease. Los Angeles, CA. (March)
• Chotani, MA; Mitra, S; Su, BY; Flavahan, S; Paris, H; Daunt, DA; Handy, DE; Clark, KR; Flavahan, NA, Presenter. 2000. Regulation of alpha2C-adrenergic receptor expression in vascular smooth muscle cells. Presented at ASMBM/ASPET 2000 Meeting. Boston, MA. (May)
• Chotani, MA, Seminar Presenter. 1988. Use of ordered deletions in place of shotgun closing, and dideoxy sequencing of the mouse VL30 gene cloned in the pUC119 plasmid vector. Presented at Senior Independent Thesis presentation. The College of Wooster. Wooster, OH.
• Mihai, C; Chotani, MA; Iscru, DF; Garbellini, D; Elton, TS; Agarwal, G, Presenter. 2006. Investigating DDR1 oligomerization at the single molecule level. Presented at Fifth Annual Ohio State University Medical Center Graduate & Postgraduate Research Day. Columbus, OH. (March)